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LentiGuide-TagRFP-2A-BSD

Plasmid

#167930

Purpose

Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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LES2A-CRE

Plasmid

#167933

Purpose

Lentiviral CRE expression tracked by mScarlet-I fluorescence marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Insert

mScarlet-P2A-CRE

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuide-TagBFP-2A-BSD

Plasmid

#167929

Purpose

Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBY2982

Plasmid

#23220

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

BirA-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pCR1265

Plasmid

#111094

Purpose

lentiviral cDNA expression vector: plenti-x1-neo-linker-IRES-Thy1.1

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMB30

Plasmid

#122247

Purpose

Lentivirus delivery for stable expression of Lb crRNA, has LbDR; Cloning vector for expression of LbCas12a crRNA. It contains BsmBI site for cloning.

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Type

Empty backbone

Use

Lentiviral

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO SwiChRca-TS-EYFP

Plasmid

#55631

Purpose

Cre-Dependent Chloride Channel

Depositor

Karl Deisseroth

Article

Berndt et al Science. 2014 Apr 25;344(6182):420-4. do

Insert

iC1C2-TS-EYFP

Use

AAV and Cre/Lox

Tags

EYFP

Expression

Mammalian

Mutation

C128A

Promoter

Ef1a

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pUNIV backbone

Plasmid

#24705

Depositor

Cynthia Czajkowski

Article

Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

Type

Empty backbone

Use

Xenopus oocyte expression

Expression

Bacterial, Mammalian, and Y…

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pCR1326

Plasmid

#111131

Purpose

Expresses UBE2T.

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

UBE2T (UBE2T Human)

Use

Lentiviral

Tags

HA

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pFsynW TeTx-LC

Plasmid

#197284

Purpose

Encodes for viral expression of the light chain of tetanus toxin.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

Tetanus toxin light chain

Use

Lentiviral

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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hRIP3 GFP RHIM mut

Plasmid

#41385

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Mutation

RHIM mutant (AAAA-459-462)

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only
hRIP1 HA GFP wt

Plasmid

#41396

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP1 (RIPK1 Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

E96G

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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hRIP3 GFP D160N

Plasmid

#41386

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Kinase Domain Mutant (D160N)

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pLEX_307_ms2_VPR

Plasmid

#79376

Purpose

Recruits VPR to compatible gRNAs for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-VPR

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCR1325

Plasmid

#111132

Purpose

Expresses USP1.

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

USP1 (USP1 Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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FepA

Plasmid

#26039

Depositor

Johann Deisenhofer

Article

Buchanan et al Nat Struct Biol. 1999 Jan . 6(1):56-63.

Insert

FepA (fepA E Coli)

Expression

Bacterial

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pLEX_307_ms2_p65_hsf1

Plasmid

#79374

Purpose

Recruits p65_hsf1 to compatible gRNAs for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-p65-hsf1

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMB4

Plasmid

#122246

Purpose

Lentivirus delivery for stable expression of As crRNA, has AsDR; Cloning vector for expression of AsCpf1 crRNA. It contains BsmBI site for cloning.

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Type

Empty backbone

Use

Lentiviral

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-SpSuc1

Plasmid

#162558

Purpose

Expresses GST-tagged S.pombe Suc1 in E.coli

Depositor

Iain Hagan

Article

Singh et al Methods Mol Biol. 2021;2329:123-142. doi

Insert

Schizosaccharomyces pombe Suc1 (suc1 Fission Yeast)

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB8381

Plasmid

#126915

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and tHMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and tHMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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