We narrowed to 10,398 results for: 158

1. pGECPL.BC10.MG

   Plasmid

   #223822

   Purpose

   Contains Cas9-editable barcode, marking guide (MG) for lineage tracing, Cre for Cas9 activation and loxP-mediated gene deletion, and Firefly Luciferase (FLuc) for live luminescence-based monitoring.

   Depositor

   Dawid Nowak

   Article

   Serio et al Cancer Discov. 2024 Jul 5. doi: 10.1158/

   Insert

   Ef1a-Driven Cre-P2A-FLuc
   

   Use

   CRISPR, Cre/Lox, Lentiviral, Luciferase, and Mous…

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPU-huMDA5-miR30-L1221

   Plasmid

   #174223

   Purpose

   All-in-one huMDA5 rescue-control shRNA knockdown vector

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   MDA5 (IFIH1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSD139A

   Plasmid

   #218129

   Purpose

   Hybrid circuit displaying hsCRBN-W384A,Y386A bait (P22 anchor) and driving expression of luxAB

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Inserts

   CRBN (CRBN Human)
   luxAB
   

   Use

   Tags

   scP22

   Expression

   Mutation

   W384A, Y386A

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#3

   Plasmid

   #208389

   Purpose

   Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#3 gene.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   sgZBP1 (Zbp1 Mouse)
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF3088

   Plasmid

   #144564

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   E4F1 (E4F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N3-CYB5R3 deltaN26

   Plasmid

   #204568

   Purpose

   Expresses EGFP-tagged CYB5R3 deltaN26 in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   CYB5R3 (CYB5R3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   delta2-26aa

   Promoter

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry-EGFP-N3-CYB5R3 K214R

   Plasmid

   #204570

   Purpose

   Expresses mCherry-EGFP-tagged CYB5R3 K214R in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   CYB5R3 (CYB5R3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K214R

   Promoter

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-EGFP-N3-CYB5R3

   Plasmid

   #204569

   Purpose

   Expresses mCherry-EGFP-tagged CYB5R3 in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   CYB5R3 (CYB5R3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA528 IST1, residues 315-360, no Cys

   Plasmid

   #180643

   Purpose

   Bacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   residues 315-360

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF2331

    Plasmid

    #143587

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CIZ1 (CIZ1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF1693

    Plasmid

    #143147

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    E4F1 (E4F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TFORF1694

    Plasmid

    #143148

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    E4F1 (E4F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF2330

    Plasmid

    #142930

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CIZ1 (CIZ1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFORF2327

    Plasmid

    #142434

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CIZ1 (CIZ1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEJS1831 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Fah

    Plasmid

    #199264

    Purpose

    Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model

    Depositor

    Erik Sontheimer

    Article

    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

    Insert

    NmeABE8e
    

    Use

    AAV and CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pD649-HAsp-CEACAM18-COMP5AP-AviTag-9xHis

    Plasmid

    #157361

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CEACAM18 (CEACAM18 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. His-MBP-IST1 N-terminal domain

    Plasmid

    #184805

    Purpose

    Bacterial expression of IST1 N-terminal domain

    Depositor

    James Hurley

    Article

    Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    aa 1-187 only

    Promoter

    T7

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIG-734pUC19-tNGFR-P2A-PIK3R1

    Plasmid

    #186107

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIG-720pUC19-tNGFR-P2A-ZAP70

    Plasmid

    #186120

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    ZAP70 (ZAP70 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIG-735pUC19-tNGFR-P2A-PIK3CD

    Plasmid

    #186087

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pIG-727pUC19-tNGFR-P2A-WASP

    Plasmid

    #186089

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    WASP (WAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pIG-714pUC19-tNGFR-P2A-SAP

    Plasmid

    #186091

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    SAP (SH2D1A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTR-459 tNGFR-P2A-STAT6

    Plasmid

    #186070

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    STAT6 (STAT6 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTR-458 tNGFR-P2A-STAT2

    Plasmid

    #186076

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    STAT2 (STAT2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET15b MOSPD2 [315-445]

    Plasmid

    #186474

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [315-445]

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1(+)-PKAcat(G1A)-mCherry

    Plasmid

    #181852

    Purpose

    mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    PKAcat-mCherry (PRKACA Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Glycine residue immediately after start site in P…

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pS97splitRBP_S1dC2

    Plasmid

    #183769

    Purpose

    Expresses SHIP1-delta-C2-Enzyme domain flanked by RBP

    Depositor

    William Kerr

    Article

    Sudan et al Front Immunol. 2022 May 4;13:830961. doi

    Insert

    SHIP1deltaC2 (INPP5D Human)
    

    Use

    Tags

    6HIS, RBP (C-terminal) w/HRV3C cleavage site, and…

    Expression

    Bacterial

    Mutation

    SHIP1deltaC2 includes aa N393 to aa Q730 only

    Promoter

    T7lac

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET24TEV-tSHIP1

    Plasmid

    #183770

    Purpose

    Expresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and 6HIS tags from pET24 backbone

    Depositor

    William Kerr

    Article

    Sudan et al Front Immunol. 2022 May 4;13:830961. doi

    Insert

    SHIP1 (INPP5D Human)
    

    Use

    Tags

    6HIS and Tobacco Etch Virus (TEV) Cleavage site

    Expression

    Bacterial

    Mutation

    includes aa1 to aa883 only

    Promoter

    T7 lac

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pS97splitRBP_S1-Enz

    Plasmid

    #183767

    Purpose

    Expresses SHIP1-Enzyme domain flanked by RBP

    Depositor

    William Kerr

    Article

    Sudan et al Front Immunol. 2022 May 4;13:830961. doi

    Insert

    SHIP1 (INPP5D Human)
    

    Use

    Tags

    6HIS, RBP (C-terminal) w/HRV3C cleavage site, and…

    Expression

    Bacterial

    Mutation

    includes aa N393 to aa L864 only

    Promoter

    T7lac

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET24TEV-tSHIP2

    Plasmid

    #183771

    Purpose

    Expresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backbone

    Depositor

    William Kerr

    Article

    Sudan et al Front Immunol. 2022 May 4;13:830961. doi

    Insert

    SHIP2 (INPPL1 Human)
    

    Use

    Tags

    6HIS and Tobacco Etch Virus (TEV) Cleavage site

    Expression

    Bacterial

    Mutation

    includes aa18 to aa985 only

    Promoter

    T7 lac

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge