We narrowed to 8,094 results for: Dos;

1. pAAV-hSyn-FAS-GCaMP6f-WPRE

   Plasmid

   #216277

   Purpose

   AAV vector with hSynapsin promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in neurons that express Cre recombinase)

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   GCaMP6f
   

   Use

   AAV and Cre/Lox

   Tags

   6XHIS and Xpress

   Expression

   Mutation

   Promoter

   human synapsin 1 (hSyn)

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV8-cPE-N

   Plasmid

   #183538

   Purpose

   Deliver a split compact Prime Editor in vivo

   Depositor

   Wen Xue

   Article

   Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

   Insert

   N-terminal fragment of SpCas9 nickase (H840A)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. ChR2-2A-Halo

   Plasmid

   #22047

   Purpose

   Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity

   Depositor

   Edward Boyden

   Article

   Han et al Front Mol Neurosci. 2009 Aug 27:2:12. do

   Insert

   ChR2-GFP-2A-Halo-YFP
   

   Use

   Tags

   GFP and YFP

   Expression

   Mammalian

   Mutation

   Note that there is a S147R mut in YFP. This mutat…

   Promoter

   CMV

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGAMA-empty

   Plasmid

   #74755

   Purpose

   Mammalian lentiviral vector

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_PCMT1_PCMT

   Plasmid

   #109932

   Purpose

   Protein expression and purification of PCMT1_PCMT

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PCMT1_PCMT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKERep

   Plasmid

   #211826

   Purpose

   Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKESa

   Plasmid

   #211828

   Purpose

   Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Rtn4a-mNeonGreen

   Plasmid

   #169032

   Purpose

   Express human Rtn4a

   Depositor

   Gia Voeltz

   Article

   Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

   Insert

   Rtn4a (RTN4 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. NPY-pHTomato

   Plasmid

   #83501

   Purpose

   Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

   Depositor

   Sebastian Barg

   Article

   Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

   Insert

   NPY (NPY Human)
   

   Use

   Tags

   pHTomato

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-28c(+)-YTHDF3

    Plasmid

    #102276

    Purpose

    For bacterial expression of His-tagged human YTHDF3

    Depositor

    Samie Jaffrey

    Article

    Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

    Insert

    YTHDF3 (YTHDF3 Human)
    

    Use

    Tags

    6X HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_NR1H3_Hormone-recep

    Plasmid

    #109950

    Purpose

    Protein expression and purification of NR1H3_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR1H3_Hormone-recep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PGEX-NEDD4

    Plasmid

    #45043

    Depositor

    Shiaw-Yih Lin

    Article

    Yim et al Cancer Cell. 2009 Apr 7;15(4):304-14. do

    Insert

    NEDD4-1 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 6, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1-mCherry-CyrCR1.mutant

    Plasmid

    #128746

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 point mutations disrupting the miR-7 site

    Promoter

    CMV

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3-basic-hRARβP-FL-luciferase

    Plasmid

    #135447

    Purpose

    Human full-length promoter of retinoic acid receptor-beta gene in pGL3-Basic-luciferase plasmid vector

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Insert

    Full-length promoter of human retinoic acid receptor-beta gene
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Human RARβ promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. All_in_one_CRISPR/Cas9_LacZ

    Plasmid

    #74293

    Purpose

    "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening

    Depositor

    Lynne Postovit

    Article

    Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do

    Inserts

    LacZ-alpha
    Cas9
    mCherry
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Lac promoter, SV40, and T7 promoter

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti6.3-SlugCas9-BlastR

    Plasmid

    #220965

    Purpose

    Expresses human codon-optimized SlugCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    SlugCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-p38beta

    Plasmid

    #86833

    Purpose

    mammalian expression of GFP-p38beta

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    p38beta (MAPK11 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUASTLOTattB_VHH-GFP4::CD8::mCherry

    Plasmid

    #163917

    Purpose

    Expression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancers

    Depositor

    Markus Affolter

    Article

    Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

    Insert

    Fusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6.3-SaCas9-BlastR

    Plasmid

    #220966

    Purpose

    Expresses human codon-optimized SaCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    SaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5_tAKARβ

    Plasmid

    #119914

    Purpose

    Mammalian expression of the tAKARβ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARβ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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