We narrowed to 23,145 results for: REP
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Plasmid#172638PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286I) and mCherry in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseLentiviralTags2xFLAG-2xSTREPExpressionMammalianMutationThr286IlePromoterCMVAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-2xFLAG-2xSTREP-CCND1(P287L)-IRES-mCherry
Plasmid#172636PurposeLentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287L) and mCherry in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseLentiviralTags2xFLAG-2xSTREPExpressionMammalianMutationPro287LeuPromoterCMVAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r
Plasmid#162937PurposeGateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoterDepositorInsertHis6 fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E42: attB5r-RU5u-Strep2-nostop-attB1r
Plasmid#162961PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Strep2-tagged fusion proteinsDepositorInsertStrep2 purification tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)
Plasmid#62580PurposeTranslational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.DepositorInsertSPRED1 (SPRED1 Human)
UseLuciferaseMutationmiR-21 binding site 1 mutant (TAAGCTA --> TAGA…Available SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-SPRED1-3'UTR (containing miR-21 sites)
Plasmid#62579PurposeTranslational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2)DepositorInsertSPRED1 (SPRED1 Human)
UseLuciferaseAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)
Plasmid#62581PurposeTranslational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.DepositorInsertSPRED1 (SPRED1 Human)
UseLuciferaseMutationmiR-21 binding site 2 mutant (TAAGCTA --> TAGA…Available SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
DNA repair library with 336 sgRNAs
Pooled Library#177664PurposeCRISPR-interference (CRISPRi) library of 366 sgRNAs targeting 118 genes encoding factors involved in DNA repair and associated processesDepositorSpeciesHomo sapiensUseCRISPRAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
HUHe variant click editor (CE1) - pCMV-T7-RepBM-nCas9-EcKlenow (JO606)
Plasmid#208949PurposeA variant CE1 construct with RepBM HUH endonuclease, expressed from CMV or T7 promoters.DepositorInsertRepBm-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC13N-TMEM192-mGFP-3xHA-T2A-TOM20-mTagBFP-TwinStrep
Plasmid#247977PurposeKnock-in vector to express TMEM192-mGFP-3xHA and TOM20-mTagBFP-twinStrep from CLYBL siteDepositorTags3xHA, TwinStrep, mGFP, and mTagBFPExpressionMammalianPromoterCAGAvailable SinceJune 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBR25 CFAP36 [L438E, K442E]-3C-mStayGold-2Strep crBSDr (C. reinhardtii)
Plasmid#238227PurposeCRISPR donor plasmid for C-terminal tagging and knock-in of CFAP36 [L438E, K442E], and insertion of Blasticidin resistance (C. reinhardtii)DepositorInsertCFAP36 [L438E, K442E]
UseCRISPR; DonorTags-3C-mStayGold-2StrepAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBR25 CFAP36 [A493E, K495A]-3C-mStayGold-2Strep crBSDr (C. reinhardtii)
Plasmid#238228PurposeCRISPR donor plasmid for C-terminal tagging and knock-in of CFAP36 [A493E, K495A], and insertion of Blasticidin resistance (C. reinhardtii)DepositorInsertCFAP36 [A493E, K495A]
UseCRISPR; DonorTags-3C-mStayGold-2StrepAvailable SinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pXTK-BB7 [Backbone_repABC-pMlb-KanR-TetR-PaqCI-Compatible_PartTypeBB]
Plasmid#229393PurposeXanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_repABC-pMlb-KanR-TetR-PaqCI-Compatible_PartTypeBB and an sfGFP dropoutDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G, T805C, C813S)-Strep
Plasmid#169749PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, T805C, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
TagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to Glycine, Threonine 805 to Cysteine…Available SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G, G808C, C813S)-Strep
Plasmid#169750PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
TagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to Glycine, Glycine 808 to Cysteine, …Available SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrep
Plasmid#180811PurposeExpression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrepDepositorInsertGST-BTLAint (AA190-289, Y257F, Y282F)-TwinStrep (BTLA Human)
ExpressionBacterialMutationBTLA (Y257F, Y282F)PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, S712C, P801G, C813S)-Strep
Plasmid#164445PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep (PDCD6IP Human)
TagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationSerine 712 to Cysteine, Proline 801 to Glycine, C…Available SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, A756C, P801G, C813S)-Strep
Plasmid#164446PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep (PDCD6IP Human)
TagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationAlanine 756 to Cysteine, Proline 801 to Glycine, …Available SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G, C813S, S863C)-Strep
Plasmid#164447PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep (PDCD6IP Human)
TagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to Glycine, Cysteine 813 to Serine, S…Available SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-RASA1-3'UTR (mut - miR-206 site)
Plasmid#62576PurposeTranslational Luciferase Reporter containing the mutated 3'UTR of RASA1. The miR-206 binding site was mutated.DepositorInsertRASA1 (RASA1 Human)
UseLuciferaseTagsNoneMutationmiR-206 binding site mutated (ACATTCCA --> AAC…Available SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only