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JK305: pMVP/Blast-DEST

Plasmid

#121855

Purpose

pMVP destination vector, empty expression vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pProEx-Htb-cytATL2-6Gly-sfGFP

Plasmid

#124065

Purpose

E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

cytATL-sfGFP

Tags

sfGFP

Expression

Bacterial

Promoter

trc

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317H)

Plasmid

#122269

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Histidine

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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hC73S-HA

Plasmid

#111529

Purpose

Expresses human KCNB1 C73S in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44.

Insert

human C73S-Kv2.1 + HA (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

C73S

Promoter

CMV

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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hC73A-HA

Plasmid

#111526

Purpose

Expresses human KCNB1 C73A in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44.

Insert

human C73A-Kv2.1 + HA (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

C73A

Promoter

CMV

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc

Plasmid

#91935

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1d

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc

Plasmid

#91933

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1b

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1b(C94S)-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag 3B/myc-M87L195V

Plasmid

#87721

Purpose

Expresses myc-M87L195V spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Tags

Myc

Expression

Mammalian

Mutation

M87 isoform (deletion of nt 1-490 - 5'UTR an…

Promoter

CMV

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/S46X without 5'UTR

Plasmid

#87730

Purpose

Expresses S46X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

S46X; 5'UTR deleted (1-190 bp)

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/S44L without 5'UTR

Plasmid

#87728

Purpose

Expresses S44L spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

S44L; 5'UTR deleted (1-190 bp)

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/L195V without 5'UTR

Plasmid

#87726

Purpose

Expresses L195V spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

L195V; 5'UTR deleted (1-190 bp)

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/E112K without 5'UTR

Plasmid

#87724

Purpose

Expresses E112K spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

E112K; 5'UTR deleted (1-190 bp)

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag 3B/myc-M87E112K

Plasmid

#87720

Purpose

Expresses myc-M87E112K spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Tags

Myc

Expression

Mammalian

Mutation

M87 isoform (deletion of nt 1-490 - 5'UTR an…

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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Human genome-wide lentiviral CRISPR gRNA library version 1

Pooled Library

#67989

Purpose

Knockout library targeting 18,010 human genes. gRNAs were improved using a new design pipeline and improved gRNA scaffold.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Species

Homo sapiens, Mus musculus, and Synthetic

Use

CRISPR and Lentiviral

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only
Mouse genome-wide lentiviral CRISPR gRNA library version 2

Pooled Library

#67988

Purpose

Knockout library targeting 18,424 mouse genes. gRNAs were improved using a new design pipeline and improved gRNA scaffold.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Species

Homo sapiens, Mus musculus, and Synthetic

Use

CRISPR and Lentiviral

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only
pT3-N90-beta-catenin

Plasmid

#31785

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

beta-catenin (CTNNB1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of aa 1-90- Constitutitvely Active

Promoter

EF1alpha

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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JN202: pMVP/SB/Blast-DEST

Plasmid

#121864

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-MOB1A

Plasmid

#172988

Purpose

constitutive expression of FLAG-tagged MOB1A

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-MOB1A (MOB1A Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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YAP/TAZ tdTomato TEAD Binding Reporter

Plasmid

#228554

Purpose

Encodes a tdTomato fluorescent protein gene driven by a TEAD binding synthetic promoter. Used to track YAP/TAZ signaling pathway activity.

Depositor

Cole DeForest

Article

Kopyeva et al Adv Mater. 2024 Sep 6:e2404880. doi: 10.

Insert

tdTomato

Use

Lentiviral

Expression

Mammalian

Promoter

GTIIC TEAD-Binding Responsive Element

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pdCas12f1_CBE-LVA

Plasmid

#220994

Purpose

Prokaryotic and constitutive expression of AsCas12f1/APOBEC1-LVA fusion

Depositor

John van der Oost

Article

Swartjes et al iScience. 2025 Nov 12;28(12):114033. doi

Insert

Acidibacillus sulfuroxidans Cas12f1-CBE

Use

CRISPR

Tags

LVA

Expression

Bacterial

Mutation

D225A

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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