We narrowed to 17,056 results for: igh

1. pET28a(+)-NEDD4L_W3-W4-HECT (492)

   Plasmid

   #187709

   Purpose

   Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 492-975 (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-491

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a(+)-NEDD4L_W4-HECT

   Plasmid

   #187710

   Purpose

   Express his-tagged NEDD4L W4-HECT in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 543-975 (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-542

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX6P1-NEDD4L_FL-CS

   Plasmid

   #187713

   Purpose

   Express the catalytically inactive GST-tagged NEDD4L FL in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL with C874 mutated to a serine (NEDD4L Human)
   

   Use

   Tags

   GST-HRV 3C site

   Expression

   Bacterial

   Mutation

   Cys874 mutated to a serine

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX6P1-NEDD4L_W2-W3-W4-HECT (348)

   Plasmid

   #187716

   Purpose

   Express GST-tagged NEDD4L W2-W3-W4-HECT (348) in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L with residues XX (NEDD4L Human)
   

   Use

   Tags

   GST-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-347

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX6P1-NEDD4L_WW34

   Plasmid

   #187718

   Purpose

   Express GST-tagged NEDD4L WW34 tandem domains in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 492-581 (NEDD4L Human)
   

   Use

   Tags

   GST-HRV 3C site

   Expression

   Bacterial

   Mutation

   only residues 492-581

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX6P1-NEDD4L_HECT

   Plasmid

   #187719

   Purpose

   Express GST-tagged NEDD4L HECT domain in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 594-975 (NEDD4L Human)
   

   Use

   Tags

   GST-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-593

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. H6Gb1-CREB(1-280) C90A, T172C

   Plasmid

   #217983

   Purpose

   bacterial expression of CREB1 domains

   Depositor

   Peter Wright

   Article

   Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   CREB1 (Creb1 Rat)
   

   Use

   Tags

   His6-GB1-tev

   Expression

   Bacterial

   Mutation

   C90A, T172C

   Promoter

   T7

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. H6Gb1-CREB(1-280) T160C

   Plasmid

   #220233

   Purpose

   bacterial expression of CREB1 domains

   Depositor

   Peter Wright

   Article

   Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   CREB1 (Creb1 Rat)
   

   Use

   Tags

   His6-GB1-tev

   Expression

   Bacterial

   Mutation

   T160C

   Promoter

   T7

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. H6Gb1-CREB(85-160)

   Plasmid

   #217984

   Purpose

   bacterial expression of CREB1 domains

   Depositor

   Peter Wright

   Article

   Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   CREB1 (Creb1 Rat)
   

   Use

   Tags

   His6-GB1-tev

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. H6Gb1-CREB(1-100)

    Plasmid

    #217978

    Purpose

    bacterial expression of CREB1 domains

    Depositor

    Peter Wright

    Article

    Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. H6GB1-CREB(1-280) C90A

    Plasmid

    #217982

    Purpose

    bacterial expression of CREB1 domains

    Depositor

    Peter Wright

    Article

    Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    C90A

    Promoter

    T7

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. H6Gb1-CREB(1-280) A50C,C90A

    Plasmid

    #217985

    Purpose

    bacterial expression of CREB1 domains

    Depositor

    Peter Wright

    Article

    Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    A50C, C90A

    Promoter

    T7

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. H6Gb1-CREB(1-280)

    Plasmid

    #217981

    Purpose

    bacterial expression of CREB1 domains

    Depositor

    Peter Wright

    Article

    Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. H6Gb1-CREB(160-280)

    Plasmid

    #217980

    Purpose

    bacterial expression of CREB1 domains

    Depositor

    Peter Wright

    Article

    Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. B-HeFSpCas9-A695Q

    Plasmid

    #207368

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A695Q
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PET-B-HypaR-SpCas9-NLS-6xHis

    Plasmid

    #207388

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HypaR-SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R661A, N692A, M694A, Q695A, H698A, amino acids 10…

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. B-HeFSpCas9-A926Q

    Plasmid

    #207370

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A926Q
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. B-evoSpCas9-E526K

    Plasmid

    #207365

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9-E526K
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    M495V, Y515N, R661Q, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. B-evoSpCas9-V495M

    Plasmid

    #207363

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9-V495M
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Y515N, K526E, R661Q, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. B-HeFSpCas9-A1060R

    Plasmid

    #207372

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A1060R
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …

    Promoter

    CBh

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge