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pANY6

Plasmid

#112198

Purpose

This plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.

Depositor

Yingfeng An

Article

Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

Type

Empty backbone

Tags

ELP-tag

Expression

Bacterial

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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MC177VSG221RNAi

Plasmid

#59730

Purpose

For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

Depositor

Gloria Rudenko

Article

Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

Insert

VSG221 RNAi fragment

Use

RNAi

Promoter

opposing T7 promoters

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAd5-B7-deltaE4

Plasmid

#122560

Purpose

Modified block 7, with deletion in E4

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 30888-35938

Use

Synthetic Biology

Mutation

Adenovirus sequences 32874-35461 replaced with Sp…

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pNM3814

Plasmid

#154122

Purpose

mec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::rtetR-L-QFAD::act-4 '3

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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MoFGFR2c-ires neo

Plasmid

#86492

Purpose

mamalian cell expression of FGFR2c

Depositor

David Ornitz

Article

Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

Insert

FgfrR2 IIIc 15D6 (Fgfr2 Mouse)

Expression

Mammalian

Promoter

Moloney murine leukemia virus LTR

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pTub-Wg

Plasmid

#64321

Purpose

Expresses Drosophila Wingless protein under the tubulin promotor

Depositor

Roel Nusse

Article

Ching et al J Biol Chem. 2008 Jun 20;283(25):17092-8

Insert

Wingless (wg Fly)

Use

Drosophila expression

Promoter

alpha-1 tubulin promoter

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pmTUBB3

Plasmid

#31236

Depositor

Cliff Ragsdale

Article

Grove et al Development. 1998 Jun . 125(12):2315-25.

Insert

TUBB3 (Tubb3 Mouse)

Use

Cdna for in situ hybridization

Mutation

3'UTR sequence

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pMJ329: pCMV-MmeFz2

Plasmid

#205266

Purpose

Human expression vector for human codon optimized MmeFz2

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

MmeFz2

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_H2_beta_CM

Plasmid

#184870

Purpose

pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

March 2, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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piggybac-Syn-mScn8a-3xV5

Plasmid

#182554

Purpose

Expresses Nav1.6 tagged with 3xV5

Depositor

Zhe J. Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Insert

Scn8a (Scn8a Mouse)

Tags

3xV5

Expression

Mammalian

Promoter

Syn

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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piggybac-Syn-mScn8a-3xHA

Plasmid

#182555

Purpose

Expresses Nav1.6 tagged with 3xHA

Depositor

Zhe J. Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Insert

Scn8a (Scn8a Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

Syn

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Limk1

Plasmid

#163414

Purpose

Expresses LIMK1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Limk1 (Limk1 Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pWVR5

Plasmid

#167509

Purpose

Broad-Host range expression vector, pSa origin, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFNR5

Plasmid

#149483

Purpose

Broad-Host range expression vector, RSF1010 origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

NahR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PSalTTC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBNG2

Plasmid

#149473

Purpose

Broad-Host range expression vector, pBBR origin, NahR(AM) repressor, PSalTTC promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

NahR(AM)-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PSalTTC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+FucciOrange

Plasmid

#128427

Purpose

Contains insert allowing in-vivo visualization of Cdk1 protein (G1)

Depositor

Rusty Lansford

Article

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

FucciOrange

Expression

Mammalian

Promoter

CMV & SP6

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR_hEMSY

Plasmid

#125156

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

EMSY (EMSY Human)

Use

Gateway Cloning

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pIS1

Plasmid

#74887

Purpose

targeting vector for C-terminal eGFP tag on Drosophila melanogaster Act5C

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Inserts

Act5C upstream homology region
Act5C downstream homology

Tags

eGFP

Expression

Insect

Available Since

April 28, 2016

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-REP

Plasmid

#64966

Purpose

mini-Tn7 vector with oriR6K, for attTn7 site determination

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

oriR6K

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET-22HT-MSI2(8-193)

Plasmid

#60356

Purpose

Express the RBD of human MSI2 in bacterial cells for protein purification

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

human MSI2 (8-193) (MSI2 Human)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains amino acids 8-193 of MSI2

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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