We narrowed to 31,202 results for: uros

1. N-term Hec1-targeted Aurora B FRET sensor

   Plasmid

   #45239

   Purpose

   sensor targeted to N-terminus of Hec1

   Depositor

   Michael Lampson

   Article

   Welburn et al Mol Cell. 2010 May 14;38(3):383-92. doi:

   Insert

   Hec1, Aurora B FRET sensor
   

   Use

   Tags

   Aurora B FRET sensor and Hec1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1358 pBabe puroL FKHR AAA delta DB

   Plasmid

   #10697

   Depositor

   William Sellers

   Insert

   FKHR AAA deltaDB (FOXO1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T24A S256A S319A , deletion in aa 208-220

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

3. CAG-FNLS-T2A-EGFP-ires-puro

   Plasmid

   #121169

   Purpose

   CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.

   Depositor

   Timo Otonkoski

   Insert

   FNLS-T2A-EGFP-ires-puro
   

   Use

   CRISPR

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-FLAG-2xSTREP-CCND2(T280A)-Puro

   Plasmid

   #172625

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2(T280A) and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Thr280Ala

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRP(Exp)-CMV> ORF_924bp (Azgp1):dTomato:IRES:Puro

   Plasmid

   #110831

   Purpose

   Expresses both zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter, where the fluorescent dT reporter is fused with the ZAG protein.

   Depositor

   Ande Satyanarayana

   Article

   Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

   Insert

   Azgp1 (Azgp1 Mouse)
   

   Use

   Non-viral gene expression vector

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti CMV Puro DEST (w118-1) FITM2

   Plasmid

   #107504

   Purpose

   Expresses FITM2, puromycin resistant

   Depositor

   Alejandro Gutierrez

   Article

   Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

   Insert

   FITM2 (codon optimized)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized sequence

   Promoter

   CMV

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV N-term HA-FLAG puro BRD7 shRescue

   Plasmid

   #41936

   Depositor

   Stephen Elledge

   Article

   Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   BRD7 (BRD7 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pLVX-EF1a-EGFP-TA Bik-IRES-Puromycin

   Plasmid

   #133026

   Purpose

   Expresses EGFP-tagged tail anchor sequence of Bik

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Bcl-2-interacting killer (BIK Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-110

   Promoter

   Human elongation factor 1 alpha

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. nonphosphorylatable Cenp-B-targted Aurora B FRET sensor

   Plasmid

   #45230

   Depositor

   Michael Lampson

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   nonphos Cenp-B targeted Aurora B FRET sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   t-a mutation in substrate sequence

   Promoter

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. hPlekhm1-C/pIRES puro Glue (S778-A1056)

    Plasmid

    #73456

    Purpose

    Express human C-terminal part (Q778-A1056) Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    CBP tag, HA tag, and strep tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pLenti-VQRRA-P2A-GFP-PGK-Puro

    Plasmid

    #110864

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid

    #121171

    Purpose

    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    xFNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30

    Plasmid

    #163359

    Purpose

    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Ly-6G (Ly6g Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSUPER-retro-puro-tetO-Mek1-shRNA

    Plasmid

    #53179

    Purpose

    Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (Map2k1 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

15. Opie2-NeoR[traF]+Opie2-PuroR[dsxM]

    Plasmid

    #131618

    Purpose

    Expresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Inserts

    PuroR[dsxM]
    NeoR[traF]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Opie2

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-BRD9_BRG1BD-PGK-Puro-IRES-GFP

    Plasmid

    #75121

    Purpose

    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRG1 bromodomain

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Bromodomain replaced with the bromodomain of BRG1

    Promoter

    Available since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

17. MSCV-BRD9_BRD1BD-PGK-Puro-IRES-GFP

    Plasmid

    #75119

    Purpose

    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD1 bromodomain

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Bromodomain replaced with the bromodomain of BRD1

    Promoter

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pBabe 3XFLAG-S37A-GATA6-3XAU1 puro

    Plasmid

    #72608

    Purpose

    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutation

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Retroviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    changed serine37 to alanine

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

19. 1070 pBabe puroL PTEN 360-364A A4

    Plasmid

    #10943

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    360-364A (SSSTS to AAAAA); A4: S380A, T382A, T383…

    Promoter

    Available since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

20. integrin beta6 Y762F, Y774F pBabe puro

    Plasmid

    #13598

    Depositor

    Dean Sheppard

    Insert

    integrin beta 6 Y762F Y774F (ITGB6 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Y762F Y774F

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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