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Plasmid#13758DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertJnk3a1 (MAPK10 Human)
TagsFlagExpressionMammalianAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NW50
Plasmid#133445PurposeCovalently circularized nanodisc NW50 for studying membrane proteins and viral entry.DepositorInsertNW50
ExpressionBacterialAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-ASAP5-WPRE
Plasmid#225709PurposePan-neuronal, pan-membrane expression of the genetically encoded voltage indicator ASAP5; can be used for dendritic voltage imagingDepositorInsertASAP5
UseAAVPromoterhuman synapsinAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SPLINTR mCHERRY v1
Pooled library#179775PurposeSPLINTR (Single-cell Profiling and LINeage TRacing) is a high diversity lentiviral barcode library for tracing clonal lineages using bulk and single-cell readouts.DepositorExpressionMammalianUseLentiviralAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p5154
Plasmid#175390PurposeHigh Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)DepositorTypeEmpty backboneUseMouse Targeting; Cloning vector, to be used to de…Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-eYFP (AAV5)
Viral prep#117382-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagseYFPAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-GCaMP6f (AAV9)
Viral prep#135632-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.DepositorAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXA2
Plasmid#153110PurposeExpression of Flag tagged human FOXA2 in mammalian cellsDepositorAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPRx-Sth1Cas9-L5
Plasmid#140993PurposeSth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in MycobacteriaDepositorInsertsSth1Cas9
TetR
L5 attP
L5 Int
KanR
UseUnspecified; Integrative in mycobactreiaAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223 SARS-CoV-2 NSP9
Plasmid#141263PurposeGateway-compatible Entry vector, with insert of NSP9 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertNSP9 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCRISPR-HOT-P2A-Clover-BlastR
Plasmid#138568PurposeUniversal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertP2A-clover-BlastR
UseCRISPRAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EFIa-iTGB1
Plasmid#115799PurposeIntegrin B1 overexpressionDepositorAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p426MET25_sfpHluorin (MRV55)
Plasmid#115697Purposesuperfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)DepositorInsertsfpHluorin
ExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TOM20MTS-mCherry-EGFP-Tet-On
Plasmid#109016PurposeTet-On construct to induce expression of tandem eGFP-mCherry tagged TOM20MTS (mitochondriaDepositorInsertTOM20MTS (MTS: mitochondria targeting signal) (TOMM20 Human)
UseLentiviralTagsmCherry-eGFPExpressionMammalianPromoterCMV promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EWB-DIO-cyan pre-eGRASP(p32)
Plasmid#111589PurposeAn AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.DepositorInsertcyan pre-eGRASP(p32)
UseAAVPromoterEf1aAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Opto-CRAC version 1
Plasmid#101245PurposeGenetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signalingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLOV2 and STIM1 cytosolic fragment
TagsmCherryExpressionBacterial and MammalianPromoterCMVAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA
Plasmid#108871PurposeExpression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafishDepositorInserts5xU6:sgRNA
Cas9-t2A-GFP
UseCRISPRPromoterU6 and hsp70Available sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3 1kb prom. CD274
Plasmid#107002PurposeModified Luciferase expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD274 (CD274 Human)
UseLuciferaseExpressionMammalianAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCI-neo FlucSM EGFP
Plasmid#90171PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLuc EGFP
ExpressionMammalianMutationR188Q in FLucAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N1_hTRIM32
Plasmid#69541PurposeExpresses human TRIM32 fused to c-terminal EGFPDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttripartite motif containing 32 (TRIM32 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by