We narrowed to 9,737 results for: Ada;

1. pET22b-GFP-CPDSalI

   Plasmid

   #38257

   Depositor

   Matthew Bogyo, Aimee Shen

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Insert

   eGFP
   

   Use

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   cloned from pEGFPN3 (Clonetech)

   Promoter

   T7

   Available since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

2. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK-flag-USP19 1-1290

   Plasmid

   #78579

   Purpose

   Expression flag-USP19 1-1290 in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 1-1290 (USP19 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   mutate 1291 residue to stop condon

   Promoter

   CMV

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pMJ823

   Plasmid

   #39313

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9 (lin2744 Listeria innocua)
   

   Use

   CRISPR

   Tags

   MBP and thioredoxin-His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

5. NsvBa

   Plasmid

   #100015

   Purpose

   Electrophysiology in mammalian cells: Mammalian expression of a non-selective voltage gated ion channel and GFP

   Depositor

   Paul DeCaen, David Clapham

   Article

   DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

   Insert

   NsvBa
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3XFlag-CMV10-Flag-mMcl-1

   Plasmid

   #32978

   Depositor

   Joseph Opferman

   Article

   Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

   Insert

   Mcl-1 (Mcl1 Mouse)
   

   Use

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. px458_2A_GFP_sgRNA_ZNF598

   Plasmid

   #127121

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   gRNA ZNF598 (ZNF598 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS415_pGal-nCas9(H840A)

   Plasmid

   #79616

   Purpose

   Expresses nCas9(H840A) in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H840A for nickase Cas9

   Promoter

   pGal1

   Available since

   Sept. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. plenti-arRNA-BFP-backbone

   Plasmid

   #180221

   Purpose

   For the cloning of arRNA-expressing vectors containing BFP fluorescence marker in mammalian cells

   Depositor

   Wensheng Wei

   Article

   Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. USP19 sgRNA2

    Plasmid

    #78586

    Purpose

    delete USP19 gene in human cell

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    USP19 sgRNA2 (USP19 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pLJM1 Flag Raptor HRas25

    Plasmid

    #26637

    Depositor

    David Sabatini

    Article

    Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

    Insert

    raptor-HRas25 (RPTOR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

12. YIplac211-VPS8-HaloTagx2

    Plasmid

    #177113

    Purpose

    Expresses Vps8-HaloTagx2 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    VPS8 (VPS8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 S6K2 KR

    Plasmid

    #17730

    Depositor

    John Blenis

    Article

    Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

    Insert

    S6K2 (RPS6KB2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K99R: kinase dead mutant

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pAID1.2-CMV-CGFP-mAID

    Plasmid

    #140606

    Purpose

    Expression of OsTIR1 and mAID-GFP protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJW1354

    Plasmid

    #69490

    Purpose

    Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms

    Depositor

    Jordan Ward

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
    

    Use

    CRISPR; Template to generate cassettes for -media…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pspCas9-3'UTR-Tetra-com-vector

    Plasmid

    #132553

    Purpose

    Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Inserts

    Cas9
    sgRNA scaffold with com replacing the Tetraloop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEBG-TRAF2 (GST)

    Plasmid

    #21586

    Depositor

    John Kyriakis

    Article

    Chadee et al Mol Cell Biol. 2002 Feb . 22(3):737-49.

    Insert

    TNF receptor-associated factor 2 (TRAF2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pAID1.2-CMV-CmCherry-mAID

    Plasmid

    #140602

    Purpose

    Expression of OsTIR1 and mAID-mCherry protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Clathrin-LCa-ECFP

    Plasmid

    #21742

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

20. px459 VRER

    Plasmid

    #101716

    Purpose

    sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E and T1337R)

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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