We narrowed to 4,937 results for: psin

1. pFSW Syt1-C2B K326,327E IRES GFP

   Plasmid

   #133820

   Purpose

   Encodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Expression

   Mutation

   K326,327E

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

   Plasmid

   #121966

   Purpose

   Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Tags

   superfold GFP-GB1-NLS-iLID

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

   Plasmid

   #84561

   Purpose

   pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops

   Depositor

   Robert Singer

   Article

   Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

   Insert

   FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
   

   Use

   Lentiviral

   Tags

   Flag, Suntag, oxBFP, AID (auxin-inducible degron)

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV SYN PSAM4 GlyR IRES EGFP

   Plasmid

   #119742

   Purpose

   Chemogenetic inhibitor expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Has Service

   AAV5

   Insert

   PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F

   Promoter

   synapsin

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.hSyn.iGluSnFr.WPRE.SV40

   Plasmid

   #98929

   Purpose

   AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   iGluSnFr
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-HNRNPA1C-mCh-Cry2WT

   Plasmid

   #101226

   Purpose

   hnRNPA1(186-320) fused to mCh-Cry2WT

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   hnRNPA1(186-320) (HNRNPA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-DIO ChRmine-eYFP-WPRE

   Plasmid

   #130996

   Purpose

   Double floxed ChRmine-eYFP under the control of Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   eYFP

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. 13XLexAop2 IVS ChrimsonR mCherry_tr

   Plasmid

   #111548

   Purpose

   Optogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherry

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR_mCherry_trafficked
   

   Use

   Tags

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CaMKIIa-hChR2(H134R)-EYFP

   Plasmid

   #26969

   Purpose

   AAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

   Has Service

   AAV1, AAV2, AAV5, and AAV9

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Histidine 134 is mutated to Arginine

   Promoter

   CaMKIIa

   Available since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. CIBN-CAAX

    Plasmid

    #79574

    Purpose

    expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

    Depositor

    Olof Idevall-Hagren, Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    CIBN (CIB1 Mustard Weed)
    

    Use

    Tags

    C-terminal CAAX-box

    Expression

    Mammalian

    Mutation

    K93A, R94A, K106A, K107A (please see depositor co…

    Promoter

    CMV

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE

    Plasmid

    #130998

    Purpose

    Double floxed ChRmine-mScarlet under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV1 and AAV5

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. CRY2 mCherry Sec61B

    Plasmid

    #102246

    Purpose

    Expresses fusion of CRY2PHR with mCherry and Sec61B

    Depositor

    Bianxiao Cui

    Article

    Che et al ACS Synth Biol. 2015 Oct 16;4(10):1124-3

    Insert

    CRY2PHR-mCherry-SEC61beta (CRY2 Mustard Weed, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PR_GEF YFP (2XPDZ-YFP-LARG DH)

    Plasmid

    #80408

    Purpose

    Recruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

    Depositor

    Michael Glotzer

    Article

    Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

    Insert

    2XPDZ_YFP_LARG DH (aa 766-997) (ARHGEF12 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. 5XUAS IVS CsChrimson tdtomato_tr

    Plasmid

    #111545

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    CsChrimson_tdtomato_trafficked
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CaMKIIa-ChRmine-mScarlet-WPRE

    Plasmid

    #130990

    Purpose

    AAV vector to drive the expression of ChRmine-mScarlet under the control of CamKIIa promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA

    Plasmid

    #37090

    Purpose

    Expresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    hChr2(H134R)-mCherry
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAL113

    Plasmid

    #22471

    Purpose

    Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PhyB NT (PHYB Mustard Weed, Mouse)
    

    Use

    Synthetic Biology

    Tags

    10aaLinker-mCFP-HrasCT

    Expression

    Mammalian

    Mutation

    Y276H

    Promoter

    CMV

    Available since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

    Plasmid

    #89873

    Purpose

    expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    bArrestin2-TevC-P2A-TdTomato
    

    Use

    AAV

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    R173H in TdTomato (Please see depositor comments …

    Promoter

    Human synapsin

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-syn-dLight1.3b

    Plasmid

    #135762

    Purpose

    For expression of the genetically encoded dopamine sensor dLight1.3b

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV9

    Insert

    dLight1.3b
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-ChrimsonR-tdT

    Plasmid

    #59169

    Purpose

    Expression vector for ChrimsonR-tdTomato

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    K176R

    Promoter

    CAG

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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