We narrowed to 10,167 results for: otos

1. M-tdTom-NM

   Plasmid

   #48679

   Purpose

   Mammalian tdTomato activation reporter for NM with GTCCCCTCCACCCCACAGTG protospacer

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, NM-PAM, and tdTomato reporter. Compatible with N. meningitidis Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Sp6

   Available Since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

2. Kohinoor/pcDNA3

   Plasmid

   #67771

   Purpose

   Kohinoor for mammalian cell expression in cytosol

   Depositor

   Takeharu Nagai

   Article

   Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

   Insert

   Kohinoor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCfB8742

   Plasmid

   #126908

   Purpose

   Template for PCR amplification of pGPD1-pFBA1 double promoter biobrick for Yarrowia lipolytica

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   pGPD1 and pFBA1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET29b-ClbPpep-CHis

   Plasmid

   #48243

   Purpose

   First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbP-pep
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   First 375 amino acids of the peptidase, which con…

   Promoter

   T7

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJH1924

   Plasmid

   #179205

   Purpose

   Pnmr-1::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-7-wCherry

   Depositor

   Mei Zhen

   Article

   Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

   Insert

   UNC-7
   

   Use

   Tags

   wCherry

   Expression

   Worm

   Mutation

   Promoter

   Pnmr-1

   Available Since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PH-dL5**

   Plasmid

   #96946

   Purpose

   PLC pleckstrin homology domain linked to FAP for CALI validation

   Depositor

   Marcel Bruchez

   Article

   He et al Nat Methods. 2016 Mar;13(3):263-268. doi

   Insert

   Pleckstrin homology domain
   

   Use

   Tags

   dL5 and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pF3BGX

   Plasmid

   #138387

   Purpose

   Empty RMCE vector (phiC31 attB/loxP)

   Depositor

   Shu Kondo

   Article

   Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

   Insert

   P5' FRT3 3xP3-Venus φC31-attB loxP FRT3 P3'
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-PH-dL5**

   Plasmid

   #96947

   Purpose

   Expresses EGFP-PLC pleckstrin homology domain linked to FAP for CALI validation

   Depositor

   Marcel Bruchez

   Article

   He et al Nat Methods. 2016 Mar;13(3):263-268. doi

   Insert

   Pleckstrin homology domain
   

   Use

   Tags

   EGFP and dL5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO-mito-mCer

   Plasmid

   #185602

   Purpose

   Lentiviral construct for expression of mCerulean targeted to mitochondria

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Insert

   mito-mCer
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJC225

    Plasmid

    #176883

    Purpose

    MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_A5803

    Plasmid

    #176878

    Purpose

    High constitutive expression of Firefly Luciferase in plants

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    CmYLCV:Luc+:HSP
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMC-1-AtUbi10-6

    Plasmid

    #176871

    Purpose

    MoClo Level 0 plasmid, contains AtUbi1 promoter

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    AtUbi10
    

    Use

    Tags

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTAG2E

    Plasmid

    #176865

    Purpose

    First sgRNA in a tRNA separated array of sgRNAs

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    sgRNA_2E
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EGFP-Endosomes-13

    Plasmid

    #56433

    Purpose

    Localization: Endosomes, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Endosomes (RHOB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pRS426-TUB1

    Plasmid

    #60385

    Purpose

    Expression of yeast alpha tubulin (TUB1) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pJH2644

    Plasmid

    #179632

    Purpose

    Pnmr-1::TetanusToxin-UrSL-wCherry unc-54 3' UTR C.elegans AVA/E/D premoter IN and others expression of TetanusToxin-UrSL-wCherry

    Depositor

    Mei Zhen

    Article

    Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

    Insert

    TetanusToxin
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pnmr-1

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSET FLII12Pglu-600u

    Plasmid

    #13563

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A Y12S-ECFP-D13R
    

    Use

    Tags

    Citrine

    Expression

    Bacterial

    Mutation

    F16A ECFP insertion in binding protein

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 Tat K41A HA (P#37)

    Plasmid

    #14655

    Depositor

    Matija Peterlin

    Article

    Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

    Insert

    Tat (tat HIV)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K41A

    Promoter

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

19. CRY2clust-eGFP-2xC2-iLID

    Plasmid

    #114420

    Purpose

    anchor iLID domain to plasma membrane with slower diffusion

    Depositor

    Dorus Gadella

    Article

    Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

    Insert

    iLID domain
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Kohinoor-Actin/pcDNA3

    Plasmid

    #67776

    Purpose

    Actin-targetted Kohinoor for mammalian cell expression

    Depositor

    Takeharu Nagai

    Article

    Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

    Insert

    Kohinoor
    

    Use

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only