We narrowed to 24,167 results for: CRISPR-Cas9

1. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3

   Plasmid

   #107728

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. miniABEMax V82G nSaCas9

   Plasmid

   #135365

   Purpose

   Expression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitution

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   miniABEMax V82G nSaCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

   Plasmid

   #90086

   Purpose

   This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA

   Depositor

   Raffaella Sordella

   Article

   Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Destabilized Domain and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47868

   Purpose

   This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.

   Depositor

   James Thomson, Erik Sontheimer

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Inserts

   NmCas9
   U6pr-tracrRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and U6

   Available since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLD037-pCMV-APOBEC-Cas9(D10A)-rXRCC1

   Plasmid

   #165444

   Purpose

   Expresses ACX, rXRCC1 in mammalian cells

   Depositor

   Wei Leong Chew

   Article

   Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

   Insert

   APOBEC-nCas9-rXRCC1 (Apobec1 Streptococcus pyogenes, Rat, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJEP316-pAAV-U6SaCas9gRNA(SapI)-pA Empty Cassette

   Plasmid

   #113693

   Purpose

   U6 driven SaCas9 gRNA expression cassette without a gRNA. SapI can be used to clone in gRNAs.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9 gRNA Cassete
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #135012

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Tags

   3x Flag, 3xFLAG (N terminal on insert), NLS, and …

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1

   Plasmid

   #112261

   Purpose

   Donor plasmid with a Cas9-p300 expression cassette, a M2rtTA expression cassette, a T2A-puro selection cassette, and two gRNA recognition sequences at both ends of the whole insertion DNA fragment.

   Depositor

   Zhili Rong

   Article

   Ma et al FEBS Lett. 2018 Jun 5. doi: 10.1002/1873

   Insert

   spCas9-p300 (EP300 Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJEP308-pAAV-EFS-dSaCas9-VP64-Dio-pA

   Plasmid

   #113685

   Purpose

   An EFS driven inverted de-catalyzed SaCas9 fused to the transcription activator VP64. dSaCas9-VP64 is floxed to render the system cre-dependent.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   inverted de-catalyzed SaCas9
   

   Use

   AAV, CRISPR, Cre/Lox, and Synthetic Biology

   Tags

   NLS and VP64

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

    Plasmid

    #123611

    Purpose

    CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    nCas9-UGI-NLS-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CAG

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK-caCas9-NatMX-Neut5L-Ade2 drive

    Plasmid

    #89577

    Purpose

    caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Insert

    Ade2 gene drive
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK-caCas9-NatMX-Neut5L-Leu2 drive

    Plasmid

    #89578

    Purpose

    caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Insert

    Leu2 gene drive
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

    Plasmid

    #189632

    Purpose

    Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. MMW2993 - human expression plasmid for SpCas9 Cluster 2

    Plasmid

    #101185

    Purpose

    Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    G582A/V583A/E584A/D585A/N588A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

    Plasmid

    #71237

    Purpose

    Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A GFP
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

    Plasmid

    #140205

    Purpose

    Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Inserts

    mCherry
    sgRNA E1
    

    Use

    CRISPR and Synthetic Biology; Proof-of-concept te…

    Tags

    Expression

    Mutation

    G174D

    Promoter

    PelcA and U3

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ezh2[FL]-dCas9

    Plasmid

    #100086

    Purpose

    Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    14056 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Ezh2[FL]-dCas9-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. DNMT3A-dCas9

    Plasmid

    #100090

    Purpose

    DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    DNMT3A (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-DNMT3A-dCas9-NLS

    Expression

    Mammalian

    Mutation

    includes aa 602-912

    Promoter

    CMV

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdCas9-GFP

    Plasmid

    #181906

    Purpose

    Fluorescently tagged dead Cas9

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_Anc689-NG-nCas9

    Plasmid

    #163527

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    Anc689-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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