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His-drebrin

Plasmid

#40362

Depositor

Phillip Gordon-Weeks

Article

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only
Clover2-C1

Plasmid

#54711

Purpose

Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.

Depositor

Michael Davidson

Type

Empty backbone

Tags

Clover2

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
EF1a_CDX2_P2A_Hygro_Barcode

Plasmid

#120432

Purpose

Barcoded lentiviral vector to express CDX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

CDX2 (CDX2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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CAV1-deltaN-mEGFP

Plasmid

#27707

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

caveolin-1, N-terminal truncated (60-178) (CAV1 Canis lupus familiaris)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

N-terminal truncation with AA 1-59 missing. The r…

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

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His6-PRC1

Plasmid

#69111

Purpose

For bacterial expression of recombinant human PRC1 tagged with His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

Protein Regulator of Cytokinesis 1 (PRC1 Human)

Tags

His6

Expression

Bacterial

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

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PylRS-AS

Plasmid

#137908

Purpose

For incorporation of fluorophenylalanine derivatives

Depositor

Wenshe Liu

Article

Lee et al Chem Commun (Camb). 2016 Oct 18;52(85):1

Insert

Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair

Expression

Bacterial

Mutation

Containing N346A, C348S mutations

Promoter

pBAD

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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M-tdTom-SP

Plasmid

#48677

Purpose

Mammalian tdTomato activation reporter for SP with GTCCCCTCCACCCCACAGTG protospacer

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, SP-PAM, and tdTomato reporter. Compatible with S. pyogenes Cas9

Use

CRISPR

Promoter

Sp6

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only
pCMV Lex VP16 HA (P#1708)

Plasmid

#14593

Depositor

Matija Peterlin

Article

Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

Insert

LexA

Tags

HA and VP16

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pRL056 pDESTattB_exorh:EGFP

Plasmid

#213510

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLJC6-EV

Plasmid

#163454

Purpose

empty vector control for pLJC6

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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MT363

Plasmid

#65858

Purpose

The gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

nahG

Expression

Plant

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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HNF1B_pLX307

Plasmid

#98342

Purpose

Lentiviral expression of HNF1B

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

HNF1B (HNF1B Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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Cyan Nano-lantern/pcDNA3

Plasmid

#67660

Purpose

cyan luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Cyan Nano-lantern

Expression

Mammalian

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pGGC011

Plasmid

#48825

Purpose

Provides alcR as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

alcR

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Plasmid

#221235

Purpose

Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Use

Lentiviral

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-puro-SNAP-FLAG-CD59

Plasmid

#50374

Purpose

Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

SNAP

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCI-neo-HA-hAtg13

Plasmid

#22874

Depositor

Noboru Mizushima

Article

Hosokawa et al Autophagy. 2009 Oct . 5(7):973-9.

Insert

autophagy-related genes 13 (ATG13 Human)

Tags

HA

Expression

Mammalian

Available Since

March 25, 2010

Availability

Academic Institutions and Nonprofits only
pDY1314_RADARS_Luciferase

Plasmid

#193191

Purpose

Express Gaussia Luciferase conditional upon RNA transcript

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

RADARS Luciferase

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - AAVS1 sgRNA

Plasmid

#70661

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against AAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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mPlum-Lifeact-7

Plasmid

#54679

Purpose

Localization: Actin, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Insert

Lifeact-mPlum

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only