Addgene

We narrowed to 2,877 results for: eed

Showing: 601 - 640 of 2877 results

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pHL1756

Plasmid

#53036

Purpose

PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
tetR

Use

Synthetic Biology

Tags

RBS(st3) and glgC leader

Expression

Bacterial

Mutation

Promoter

pConNoHind and pConNoHindM12

Available since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
pRY1001

Plasmid

#21787

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

CFP-Ste12 (STE12 A. victoria, Budding Yeast)

Use

Tags

CFP

Expression

Yeast

Mutation

contains 1000 bp UTR (Ste12 promoter) upstream of…

Promoter

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-BirA-HA-Tulp3

Plasmid

#236749

Purpose

Express BirA and HA tagged TULP3 transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

Insert

Tulp3 (Tulp3 Mouse)

Use

Tags

BirA and HA

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-roxSTOProx-NaChBac-TdTomato

Plasmid

#236253

Purpose

Dre dependent NaChBac-TdTomato expression

Depositor

Janelia Viral Tools

Article

Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

Insert

NaChBac-TdTomato

Use

AAV

Tags

TdTomato

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-Zeo-miR-430

Plasmid

#221726

Purpose

Expression of Zeocin resistance marker followed by perfect miR-430 target site insertion in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR-miR-430

Use

Fish expression

Tags

Expression

Mutation

Promoter

simian CMV IE94

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPI214

Plasmid

#207440

Purpose

ErkB knock out vector

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

ErkB

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPI161

Plasmid

#207439

Purpose

Shuttle vector LifeAct-mCherry

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

LifeAct-mCherry

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pPI486

Plasmid

#207441

Purpose

6xHis-SS-TEV-ErkB bacterial expression vector

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

ErkB

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPI580

Plasmid

#207445

Purpose

6xHis-SS-TEV-ErkA bacterial expression vector

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

ErkA

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPI544

Plasmid

#207443

Purpose

6xHis-SS-TEV-ErkB-ErkB-T176A bacterial expression vector

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

ErkB

Use

Unspecified

Tags

Expression

Mutation

T176A

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPI560

Plasmid

#207444

Purpose

6xHis-SS-TEV-ErkB-Y178A bacterial expression vector

Depositor

Rob Kay

Article

Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

Insert

ErkB

Use

Unspecified

Tags

Expression

Mutation

Y178A

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-Rab29

Plasmid

#186021

Purpose

2xHis-Rab29

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

2xHis-Rab29 (RAB29 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pORE303N_CEN12Fuzzy3MYB

Plasmid

#194440

Purpose

CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12 and Fuzzy3MYB

Depositor

Chung-Jui Tsai

Article

Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

Insert

gRNA array targeting CEN1, Fuzzy3MYB

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

MtU6

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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HySp5-310

Plasmid

#192995

Purpose

Plasmid that encodes for Hydra Sp5, lacking the SP box

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Use

Tags

HA

Expression

Mammalian

Mutation

no SP box

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pORE303N_CEN1

Plasmid

#194439

Purpose

CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout CEN1

Depositor

Chung-Jui Tsai

Article

Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

Insert

gRNA targeting CEN1

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

MtU6

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGEM-T-Easy-HySp5-557

Plasmid

#193000

Purpose

Plasmid to generate an ISH probe for Hydra Sp5

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ZfSp5a-377

Plasmid

#192997

Purpose

Plasmid that encodes for Zebrafish Sp5

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Zebrafish Sp5

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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HySp5-227

Plasmid

#192996

Purpose

Plasmid that encodes for Hydra Sp5, lacking the SP box and DNA binding domain

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Use

Tags

HA

Expression

Mammalian

Mutation

no SP box and DNA binding domain

Promoter

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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HySp5:Luc

Plasmid

#192985

Purpose

Plasmid to test the Hydra Sp5 promoter activity

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5 promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ZfSp5l1

Plasmid

#192999

Purpose

Plasmid that encodes for Zebrafish Sp5-like

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Zebrafish Sp5-like

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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HySp5-337

Plasmid

#192994

Purpose

Plasmid that encodes for Hydra Sp5, lacking the DNA binding domain

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Sp5

Use

Tags

HA

Expression

Mammalian

Mutation

no DNA binding domain

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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ZfWnt3:Luc

Plasmid

#192992

Purpose

Plasmid to test the Zebrafish Wnt3 promoter activity

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Zebrafish Wnt3 promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/TARG1

Plasmid

#172580

Purpose

For generation of FlpIn lines with untagged TARG1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR/zeo-TARG1

Plasmid

#172574

Purpose

For gateway cloning of N-terminal tagged TARG1 constructs.

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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gSCPL35-mRFP

Plasmid

#181960

Purpose

SCPL35 protein tagged with mRFP, under control of native promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

SCPL35

Use

Tags

mRFP

Expression

Plant

Mutation

Promoter

native SCPL35

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF-pntAB

Plasmid

#158609

Purpose

Low phosphate induction of pntAB

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

pntAB(membrane bound proton translocating pyridine nucleotide transhydrogenase)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Insulated ugpBp from E. coli

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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mt-DdCBE G1333-C

Plasmid

#174561

Purpose

mitochondrial-targeting DdCBE (split DddA, G1333-C) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1333-C

Use

Tags

FLAG

Expression

Plant

Mutation

Promoter

PcUbi

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Sc NybbR eRF1 (G180A)

Plasmid

#175415

Purpose

Encodes His-MBP-TEV-ybbR-G180A eRF1

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF1 (SUP45 Budding Yeast)

Use

Tags

His-MBP-TEV and ybbR

Expression

Bacterial

Mutation

G180A

Promoter

T7

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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mt-DdCBE G1397-C

Plasmid

#174563

Purpose

mitochondrial-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-C

Use

Tags

FLAG

Expression

Plant

Mutation

Promoter

PcUbi

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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mt-DdCBE G1397-N

Plasmid

#174562

Purpose

mitochondrial-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-N

Use

Tags

HA

Expression

Plant

Mutation

Promoter

PcUbi

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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mt-DdCBE G1333-N

Plasmid

#174560

Purpose

mitochondrial-targeting DdCBE (split DddA, G1333-N) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1333-N

Use

Tags

HA

Expression

Plant

Mutation

Promoter

PcUbi

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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cp-DdCBE G1397-C

Plasmid

#174559

Purpose

Chloroplast-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-C

Use

Tags

FLAG

Expression

Plant

Mutation

Promoter

PcUbi

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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cp-DdCBE G1397-N

Plasmid

#174558

Purpose

Chloroplast-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-N

Use

Tags

HA

Expression

Plant

Mutation

Promoter

PcUbi

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJH775_pJH_LldR_PlldR-sfGFP.2opal

Plasmid

#163734

Purpose

Lactate-inducible expression of green fluorescent protein encoding two opal codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.2TGA

Use

Tags

Expression

Bacterial

Mutation

gfp with first two leucine codons mutated to amber

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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FoxFTVC+bpFOG_MRAS-G22V

Plasmid

#107521

Purpose

Constitutive activation of FGF/MAPK pathway by M-Ras-G22V mutant

Depositor

Lionel Christiaen

Article

Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

Insert

M-Ras protein

Use

Tags

Expression

Mutation

changed Glycine 22 to Valine

Promoter

TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pQlinkH-YbATP-grasp

Plasmid

#129639

Purpose

Expression plasmid of ATP-grasp from Y. bercovieri

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbATP-grasp

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

pQE

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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sgPYM1

Plasmid

#105249

Purpose

human PYM1 sgRNA

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

sgRNA for PYM1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only
Gal4pBS13-Pleum

Plasmid

#60332

Purpose

Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Gal4pBS2-Pleum

Plasmid

#59992

Purpose

Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
UASGAL-CU1-Pleum

Plasmid

#60331

Purpose

Contains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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