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We narrowed to 12,605 results for: LIC

Showing: 301 - 320 of 12605 results

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pMOS017E: Entry vector for: ratiometric pHluorin (cytosolic)

Plasmid

#163069

Purpose

Entry vector for: ratiometric pHluorin (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET mRuby2 LIC cloning vector (H6-mRuby2)

Plasmid

#86934

Purpose

pET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2

Depositor

Chris Jeans

Type

Empty backbone

Use

Tags

6xHis, AviTag (biotin), TEV cleavage site, and mR…

Expression

Bacterial

Mutation

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pET tdTomato LIC cloning vector (H6-tdTomato)

Plasmid

#86928

Purpose

pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato

Depositor

Chris Jeans

Type

Empty backbone

Use

Tags

6xHis, AviTag (biotin), TEV cleavage site, and td…

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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mHDAC7-Spliced (1-938) pEF6-V5 (MJS_014) [Edit]

Plasmid

#73267

Purpose

Expression of mouse HDAC7-S (1-938) in mammalian cells (V5 tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Use

Tags

His/V5

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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mHDAC7-Spliced (1-938) pEF6-Flag (MJS_015)

Plasmid

#73268

Purpose

[Edit] Expression of mouse HDAC7-S (1-938) in mammalian cells (Flag tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL100EE

Plasmid

#166798

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. LL100EE mutant is loss of CARD8 and NLRP1 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

LL100EE

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-p2A-LIC1 Y101A, Y103A

Plasmid

#74605

Purpose

expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 Y101A, Y103A (DYNC1LI1 Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

Y101A, Y103A

Promoter

SFFV

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pET mClover LIC cloning vector (H6-mClover)

Plasmid

#86931

Purpose

pET LIC cloning vector for Biotin-His6-tev-yORF-mClover

Depositor

Chris Jeans

Type

Empty backbone

Use

Tags

6xHis, AviTag (biotin), TEV cleavage site, and mC…

Expression

Bacterial

Mutation

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG E1322R

Plasmid

#166809

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

E1322R

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A

Plasmid

#166801

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

S730A

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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tau exon 9–12 FTDP-17 N279K splicing minigene

Plasmid

#122967

Purpose

expresses circular tau RNAs with the FTDP-17 N279K

Depositor

Stefan Stamm

Article

Welden et al Biochim Biophys Acta Mol Basis Dis. 2018

Insert

maps

Use

Tags

Expression

Mammalian

Mutation

FTDP-17 mutation

Promoter

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pET mClover LIC cloning vector (u-mClover)

Plasmid

#86929

Purpose

pET LIC cloning vector for yORF-mClover

Depositor

Chris Jeans

Type

Empty backbone

Use

Tags

mClover

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pET LIC cloning vector for hairpin mRNA (2U-T)

Plasmid

#29719

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

MYFQSNA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-p2A-LIC1 C-terminal half

Plasmid

#74603

Purpose

expresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherry

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

amino acids 389-523 of LIC1

Promoter

SFFV

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167848

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Use

Tags

6xHis and AviTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S KK93EE

Plasmid

#166796

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. KK93EE mutant is partial loss of CARD8 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

KK93EE

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S E597R

Plasmid

#166800

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. E597R mutant is partial loss of CARD8 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

E597R

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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mHDAC7-Unspliced (23-938) pEF6-Flag (MJS_018)

Plasmid

#73269

Purpose

mHDAC7-U (23-938) pEF6-V5 (MJS_017) (Flag tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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ZIKV isolate PRVABC59 NS3 helicase domain

Plasmid

#228664

Purpose

Bacterial expression of the Zika virus NS3 helicase

Depositor

ASAP Discovery, Frank von Delft

Insert

Zika virus NS3 helicase

Use

Tags

His-GST-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pTwist-SARS-CoV-2 Spike-deltaC18 (Pelican)

Plasmid

#212401

Purpose

Encodes SARS-CoV-2 spike D614G,Q677P for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike D614G,Q677P (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

D614G,Q677P

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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