Addgene

We narrowed to 3,628 results for: WOL

Showing: 301 - 320 of 3628 results

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pRK5-HA-SZT2

Plasmid

#87035

Purpose

mammalian expression of SZT2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-H13LTat-CD8a/d2eGFP-IRES-Nef

Plasmid

#126552

Purpose

Replication incompetent HIV with H13L Tat and d2eGFP/CD8a reporter

Depositor

Jonathan Karn

Article

Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

Insert

HIV-1 vector pNL4-3

Use

Lentiviral

Tags

CD8a and GFP

Expression

Mutation

Delta gag/pol

Promoter

HIV LTR

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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PB-TAG-ERP2

Plasmid

#80479

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)

Depositor

Knut Woltjen

Article

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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SpCas9_sgRNA_expression_in_pBluescript

Plasmid

#122089

Purpose

U6 driven SpCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9 tracr (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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PB-TET

Plasmid

#20909

Purpose

piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

Gateway Destination Vector

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-SZT2

Plasmid

#87034

Purpose

mammalian expression of SZT2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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NmCas9_sgRNA_expression_in_pBluescript

Plasmid

#122091

Purpose

U6 driven NmCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

NmCas9 tracrRNA (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSW610 - GR-LhG4_BD

Plasmid

#115992

Purpose

entry vector for GreenGate cloning method, CDS, with adaptors for B and D modules

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

GR-LhG4_BD

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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HA-UBE2E1 C131A in pcDNA3.1

Plasmid

#118214

Purpose

Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

UBE2E1 (UBE2E1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

changed cysteine 131 to alanine

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pQE30 PTBP1 isoform1

Plasmid

#108591

Purpose

Recombinant His tagged protein production

Depositor

Chris Smith

Article

Wollerton et al RNA. 2001 Jun;7(6):819-32.

Insert

PTBP1 isoform 1 (PTBP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

isoform 1 (isoform c)

Promoter

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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SaCas9_sgRNA_expression_in_pBluescript

Plasmid

#122090

Purpose

U6 driven SaCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSW614 - pREV

Plasmid

#115989

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pREV

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSW618 - pAHP6

Plasmid

#115991

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pAHP6

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSW615 - pATHB-8

Plasmid

#115990

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pATHB-8

Use

Unspecified

Tags

Expression

Mutation

A1287T to abolish Eco31I site

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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p11d-sctRPA

Plasmid

#102614

Purpose

Expression of S. cerevisiea RPA in E.coli

Depositor

Marc Wold

Article

Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-50

Inserts

RPA1 (RFA1 Budding Yeast)
RPA2 (RFA2 Budding Yeast)
RPA3 (RFA3 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

RFA2 has intron deleted

Promoter

T7

Available since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Spy CCR5-pegRNA

Plasmid

#169856

Purpose

SpyCas9-pegRNA for CCR5

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

Spy CCR5-pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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Spy EMX1-pegRNA

Plasmid

#169855

Purpose

SpyCas9-pegRNA for EMX1

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

Spy EMX1-pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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plc242-GFP-Nprl2

Plasmid

#87051

Purpose

mammalian expression of Nprl2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

Nprl2 (NPRL2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSW609 - N001

Plasmid

#115993

Purpose

intermediate vector for GreenGate cloning method, contains modules from A-F

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

H-A adapter:pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Use

Unspecified

Tags

HDEL and SP(ER)

Expression

Mutation

Promoter

Available since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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SauCas9 EMX1-pegRNA

Plasmid

#169857

Purpose

SauCas9 pegRNA for EMX1

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SauCas9 EMX1-pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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