We narrowed to 2,152 results for: gibson assembly

1. pBTK402

   Plasmid

   #110598

   Purpose

   BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   mRFP dropout (RSF1010 origin)
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBTK501

   Plasmid

   #110602

   Purpose

   BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBTK520

   Plasmid

   #110604

   Purpose

   BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (spectinomycin resistance)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBTK403

   Plasmid

   #110599

   Purpose

   BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   mRFP dropout (RSF1010 origin)
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBTK401

   Plasmid

   #110597

   Purpose

   BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   mRFP dropout (RSF1010 origin)
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBTK601

   Plasmid

   #110607

   Purpose

   BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   Cas9
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBTK519

   Plasmid

   #110603

   Purpose

   BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (kanamycin resistance)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBTK402

   Plasmid

   #110598

   Purpose

   BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   mRFP dropout (RSF1010 origin)
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBTK501

   Plasmid

   #110602

   Purpose

   BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBTK520

    Plasmid

    #110604

    Purpose

    BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (spectinomycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBTK403

    Plasmid

    #110599

    Purpose

    BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    mRFP dropout (RSF1010 origin)
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBTK401

    Plasmid

    #110597

    Purpose

    BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    mRFP dropout (RSF1010 origin)
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBTK601

    Plasmid

    #110607

    Purpose

    BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Cas9
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MZB184

    Plasmid

    #228488

    Purpose

    Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Type

    Empty backbone

    Tags

    10xHis-SUMO(Smt3)

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MZB223

    Plasmid

    #228491

    Purpose

    Empty C-terminal 8xHis tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Type

    Empty backbone

    Tags

    8xHis

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MZB116

    Plasmid

    #228480

    Purpose

    "Empty" C-terminal self-cleaving CPD-tag vector for rapid cloning/DNA Assembly via SwaI restriction enzyme site (bacterial expression)

    Depositor

    Richard Baker

    Type

    Empty backbone

    Tags

    CPD-10xHis

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MZB133

    Plasmid

    #228484

    Purpose

    "Empty" N-terminal TEV cleavable 6x His tag vector for rapid cloning/DNA Assembly via SmaI restriction site (Bacterial Expression)

    Depositor

    Richard Baker

    Type

    Empty backbone

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C77m

    Plasmid

    #120989

    Purpose

    MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    AmpR
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. C78m

    Plasmid

    #120990

    Purpose

    MoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    ChlorR
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MZB184

    Plasmid

    #228488

    Purpose

    Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Type

    Empty backbone

    Tags

    10xHis-SUMO(Smt3)

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge