We narrowed to 6,650 results for: tac

1. pXD70LacZ6-LacI-Ptac(LacO)-LacZ

   Plasmid

   #191621

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2X

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)

   Plasmid

   #110558

   Purpose

   miniTn7 delivery plasmid with lacIq-Ptac inducible promoter

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   lacIq-Ptac
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacIq-Ptac

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. hSyn-GtACR2-ChRmine-tandem-eYFP

   Plasmid

   #192580

   Purpose

   AAV-vector for bidirectional optogenetic manipulation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   EYFP- soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV Ef1a DIO GeNL_SS hGtACR1

   Plasmid

   #233511

   Purpose

   Luciferase opsin fusion protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

   Inserts

   mNeonGreen
   ISN-IE
   hGtACR1
   

   Use

   AAV

   Tags

   Gaussia luciferase signal sequence

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-fDIO-stGtACR2-FusionRed

   Plasmid

   #217975

   Purpose

   AAV transfer plasmid to express stGtACR2-FusionRed in a frt-dependent manner

   Depositor

   Edward Callaway

   Article

   Kirchgessner et al Curr Biol. 2021 Dec 6;31(23):5121-5137.e

   Insert

   stGtACR2-FusionRed
   

   Use

   AAV

   Tags

   FusionRed

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC18T-mini-Tn7T-hph-Ptac

   Plasmid

   #218364

   Purpose

   Empty backbone for Tn7-site insertion with lacIq-Ptac expression system

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

   Plasmid

   #195741

   Purpose

   Base editing for making a pre-stop codon in mmoX using phenol inducible system

   Depositor

   Seung-Goo Lee

   Article

   Jeong et al Chem Eng J, 2023

   Insert

   Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Po (phoenol-inducible promoter)

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTwist-SARS-CoV-2 Spike-deltaC18 BA.2

   Plasmid

   #212487

   Purpose

   Encodes SARS-CoV-2 variant BA.2 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike BA.2 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTwist-SARS-CoV-2 Spike-deltaC18 HK.3

   Plasmid

   #212572

   Purpose

   Encodes SARS-CoV-2 spike for HK.3 pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike HK.3 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLIK S6K (E389-deltaCT) neo

    Plasmid

    #58516

    Purpose

    Lentiviral expression vector for an inducible HA-tagged rat S6K1-E389-deltaCT

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Rps6kb1 (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T389E, stop codon at amino acid 402

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC

    Plasmid

    #13005

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 1 delta CC (SULF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Cysteine 87 to Alanine

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pRK7-HA-S6K1-F5A-E389-deltaCT

    Plasmid

    #8990

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F5A, T389E, stop codon at amino acid 402

    Promoter

    Available since

    Sept. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

13. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124543

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41

    Promoter

    Minimal ADE

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-H2B-mRuby3-IRES-eGtACR1-ST

    Plasmid

    #108960

    Purpose

    Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eGtACR1-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC

    Plasmid

    #22931

    Purpose

    Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)

    Depositor

    Alexandra Newton

    Article

    Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

    Insert

    PHLPP1-deltaC (PHLPP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PHLPP-deltaC, deletion of PDZ binding domain--the…

    Promoter

    CMV

    Available since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

16. H2A-mTurquoise-RHOA-G14V-deltaCAAX

    Plasmid

    #176097

    Purpose

    Constitutively active, nucelar localized RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    RHOA (RHOA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G14V

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. H2A-mTurquoise-RAC1-G12V-deltaCAAX

    Plasmid

    #176095

    Purpose

    Constitutively active, nuclear localized Rac1

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    RAC1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CMV.SV40.THBS1-deltaCTD-HA.SV40(polyA)

    Plasmid

    #156163

    Purpose

    Expresses truncated (AA 1-954) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Truncated THBS1 (amino acid residues 1-954)

    Promoter

    CMV

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1/Myc-His(-)-HSulf-2 deltaCC

    Plasmid

    #13006

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 2 deltaCC (SULF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Cysteine 88 to Alanine

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pCI-neo-myc-LC3(deltaC22)

    Plasmid

    #45448

    Depositor

    Tamotsu Yoshimori

    Article

    Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Encodes amino acids 1-120

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only