We narrowed to 14,321 results for: cas9

Showing: 3181 - 3200 of 14321 results

Results per page:

Sort results by:

PABE-7

Plasmid

#115628

Purpose

Targeted A to G in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Publication

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

wtTadA-TadA7.10-nCas9-3*NLS

Use

CRISPR

Expression

Plant

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pBK-EE-BE3

Plasmid

#85175

Purpose

Expresses EE-BE3 in mammalian cells

Depositor

David Liu

Publication

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

EE-BE3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

Plasmid

#222994

Purpose

Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Publication

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Promoter

pCAG

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-CMV-NLS-dCas9-NLS

Plasmid

#99908

Purpose

For the expression of NLS-dCas9-NLS

Depositor

Yujie Sun

Publication

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Insert

NLS-dCas9-NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV ORANGE Dlg4-HaloTag KI

Plasmid

#139656

Purpose

Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and HaloTag donor

Use

AAV

Expression

Mammalian

Promoter

U6

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-APIC-Cas9

Plasmid

#121657

Purpose

gateway cloning destination vector for expressing enhancer driven Cas9

Depositor

Chun Han

Publication

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

Enlarge
pEF-XMAS-1xStop

Plasmid

#164411

Purpose

Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

Depositor

Xiao Wang

Publication

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

mCherry

Use

CRISPR and Synthetic Biology

Tags

T2A-GFP

Expression

Mammalian

Promoter

EF1-alpha

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pR26 iBFP Dest

Plasmid

#74284

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and BFP, for gateway cloning

Depositor

Ralf Kuehn

Publication

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pPBT/EFS-Cas9-PuroTk

Plasmid

#170542

Purpose

Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

Depositor

Jacob Giehm Mikkelsen

Publication

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

PuroTK

Use

CRISPR

Expression

Mammalian

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
P823_eSpCas9(1.1)-RecA

Plasmid

#87264

Purpose

Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

Depositor

Yonglun Luo

Publication

Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

Insert

RecA

Tags

FLAG

Expression

Mammalian

Promoter

CBh

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pV132-vCas9

Plasmid

#203223

Purpose

Mammalian expression of vCas9

Depositor

Vikash Chauhan, Robert Langer, Phil Sharp

Publication

Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

vCas9

Use

AAV and CRISPR

Expression

Mammalian

Mutation

S55R R976A K1003A T1314R

Promoter

CBh

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pYLsgRNA-OsU6a

Plasmid

#66194

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Publication

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

OsU6a

Available since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pMO1170

Plasmid

#232073

Purpose

Expresses Adenine Base Editor for Mammalian Cells

Depositor

Franz Meitinger

Publication

Ng et al Methods Mol Biol. 2025;2872:167-187. doi

Insert

Adenine Base Editor

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1-alpha core promoter

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PEA1

Plasmid

#176527

Purpose

Delivers all prime editing nickase components in a single plasmid

Depositor

Paul Thomas

Publication

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Use

CRISPR

Expression

Mammalian

Promoter

CbH for Cas9, hU6 for gRNAs

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
HeFSpCas9

Plasmid

#92355

Purpose

Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Publication

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

Promoter

Cbh

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-hA3G-BE3

Plasmid

#113415

Purpose

Expresses hA3G-BE3 in mammalian cells

Depositor

Jia Chen

Publication

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3G-BE3 (APOBEC3G Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-NLS-AcrIIA4

Plasmid

#113037

Purpose

Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

Depositor

Dominik Niopek

Publication

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

SV40 NLS-AcrIIA4

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
pORANGE GFP-Syt7 KI

Plasmid

#131488

Purpose

Endogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Syt7, Syt7 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
BRCA1 A10.2 gRNA

Plasmid

#90549

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA1

Depositor

Iain Cheeseman

Publication

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA1 (Guide Designation A10.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pCRISPR-HOT-Clover-BlastR

Plasmid

#138569

Purpose

Universal Cleavable Donor Plasmid for tagging with Clover-BlastR using NHEJ

Depositor

Hans Clevers

Publication

Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

Insert

Clover-BlastR

Use

CRISPR

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge