Addgene

We narrowed to 48,841 results for: des.1

Showing: 3181 - 3200 of 48841 results

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pcDNA3 HA eIF4GI (1-1599)

Plasmid

#45640

Depositor

Nahum Sonenberg

Article

Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

Insert

eIF4GI transcript variant 2 (EIF4G1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pBABE puro SDF-1 alpha

Plasmid

#12270

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

SDF-1 alpha (CXCL12 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; HE1A:BFP -1

Plasmid

#180008

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; HE1A: BFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; HE1A:BFP -1

Plasmid

#180011

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (1-249)

Plasmid

#42204

Depositor

Robert Lefkowitz

Article

Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

Insert

c-SRC (1-249) (SRC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

contains amino acids 1-249

Promoter

CMV

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
LentiCRISPR v2 RB1 sgRNA #1

Plasmid

#202516

Purpose

Expressing Cas9 and sgRNA targeting human RB1 gene

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

RB1 sgRNA (RB1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (1-397)

Plasmid

#42205

Depositor

Robert Lefkowitz

Article

Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

Insert

c-SRC (1-397) (SRC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

contains amino acids 1-397

Promoter

CMV

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pclbw-opa1(isoform 1)-myc

Plasmid

#62845

Purpose

Mammalian Expression of myc-tagged Opa1 isoform1

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mmOPA1isoform1-myc (Opa1 Mouse)

Use

Retroviral

Tags

9xMyc

Expression

Mammalian

Mutation

Promoter

cmv-bactin

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
pXL002-ishRNA-beta-catenin-1

Plasmid

#36297

Depositor

Sean Palecek

Article

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of beta-catenin-1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

IRES-RFP-Puro

Expression

Mammalian

Mutation

Promoter

TetO-H1

Available since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only
MBP-CHC(1-1074)-His6

Plasmid

#100748

Purpose

Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CLTC (CLTC Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

Residues 1-1074

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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OCT4 GFP puro Donor 1

Plasmid

#22209

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-OCT-GFP-2APuro-PA (POU5F1 Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-AC8(1-106)

Plasmid

#181958

Purpose

Residues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.

Depositor

Jin Zhang

Article

Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

Insert

EGFP-AC8(1-106) (Adcy8 Rat)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Teton-puro-shOCT4

Plasmid

#198761

Purpose

conditional knockdown of OCT4

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

shOCT4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG-SLC38A9.1 (1-119aa)

Plasmid

#71862

Purpose

lentiviral stable expression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized, first 119 amino acids

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
Anti-Neuroligin-1 [N97A/31R]

Plasmid

#114554

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-1 (Rat). Derived from hybridoma N97A/31.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neuroligin-1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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XE237 Human LEF-1 pCS2+

Plasmid

#16709

Depositor

Randall Moon

Article

Li et al J Biol Chem. 2004 Jan 23;279(4):2873-84.

Insert

Human LEF-1 (LEF1 Human)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pET28-p53 (1-90) (72P)

Plasmid

#62080

Purpose

Expression of human p53 (1-90) (72P) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

contains TAD/PP residues 1-90; 72P

Promoter

T7

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
p3xFLAG-CMV TIP60 1-211

Plasmid

#78794

Purpose

To overexpress TIP60 1-211 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

TIP60 (KAT5 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

1-211

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
GST-TEV-FUS-1-373aa

Plasmid

#29631

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-373aa (FUS Human)

Use

Tags

GST and TEV

Expression

Bacterial

Mutation

Contains FUS aa# 1-373

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-6P-1-INTS3-C

Plasmid

#128417

Purpose

Express INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

INTS3 C-terminal region (514-1042 aa)

Promoter

Tac promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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