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Showing: 3181 - 3200 of 77498 results

  1. pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

    Plasmid
    #68720
    Purpose
    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.
    Depositor
    Tobias Bonhoeffer, Mark Huebener, Tobias Rose
    Article
    Rose et al Science. 2016 Jun 10;352(6291):1319-22.
    Has Service
    AAV1
    Insert
    mRuby2-P2A-GCaMP6s
    Use
    AAV
    Mutation
    Cre-dependent expression from inverted open readi…
    Promoter
    hSyn1-FLEX
    Available Since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    68720-plasmid-map-sequence-id-198989

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  4. pODN-H2BC11-mR2

    Plasmid
    #183866
    Purpose
    Repair template for the C-terminal tagging of H2B histones with mRuby2 in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    H2BC11 homology arms with linker-mRuby2 (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mRuby2
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available Since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    183866-plasmid-map-sequence-id-368215

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  5. pODN-mR2-MYH10

    Plasmid
    #183869
    Purpose
    Repair template for the N-terminal tagging of myosin heavy chain 10 with mRuby2 in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    MYH10 homology arms with mRuby2-linker (MYH10 Human)
    Use
    CRISPR; Donor template
    Tags
    mRuby2-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available Since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183869-plasmid-map-sequence-id-362448

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  6. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

    Plasmid
    #87827
    Purpose
    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    NLS
    PTS1
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87827-plasmid-map-sequence-id-175008

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  9. pSW002-Pc-TorT(sp)-mNeonGreen

    Plasmid
    #205021
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorT-mNeonGreen
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available Since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205021-plasmid-map-sequence-id-407999

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  14. RCAS-sgATG7

    Plasmid
    #75228
    Purpose
    An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh
    Depositor
    Noor Gammoh
    Article
    Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:
    Insert
    Autophagy Related 7 (Atg7 Mouse)
    Use
    Retroviral
    Promoter
    U6
    Available Since
    June 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pODN-H2BC11-TagBFP

    Plasmid
    #183867
    Purpose
    Repair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    H2BC11 homology arms with linker-mTagBFP (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mTagBFP
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available Since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183867-plasmid-map-sequence-id-362447

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Showing: 3181 - 3200 of 77498 results