We narrowed to 32,170 results for: uros

1. pLVX-Flag-Polß(D256A)-IRES-puro

   Plasmid

   #128677

   Purpose

   mammalian expression of Flag-Polß(D256A) protein with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D256A

   Promoter

   CMV

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-6NBC-GFP-PGK-Puro-Barcode1

   Plasmid

   #83678

   Purpose

   individual 6N barcode to make barcoded cell line variants

   Depositor

   Monte Winslow

   Article

   Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

   Insert

   Barcode1
   

   Use

   Retroviral

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV Puro DEST (w118-1) VOPP1

   Plasmid

   #107507

   Purpose

   expressing VOPP1, puromycin resistant

   Depositor

   Alejandro Gutierrez

   Article

   Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

   Insert

   VOPP1 (VOPP1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-HIF2α P405A/P531A/N847Q-pBabe-Puro

   Plasmid

   #25948

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 405 to Alanin…

   Available Since

   Sept. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRetroX-Tight-puro-3xFLAG-RUNX3 R122C

   Plasmid

   #203439

   Purpose

   Mammalian expression of RUNX4

   Depositor

   Yoshiaki Ito

   Article

   Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

   Insert

   RUNX3 (RUNX3 Human)
   

   Tags

   3 x FLAG

   Expression

   Mammalian

   Mutation

   R122C

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N

   Plasmid

   #22018

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mVenus-PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Rac1 starts at I4 and contains mutation T17N.

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pLenti-C-Myc-DDK-IRES-Puro HNRNPR

   Plasmid

   #200730

   Purpose

   Lentiviral expression of Flag-Tagged human HNRNPR

   Depositor

   Claudio Alarcón

   Insert

   HNRNPR (HNRNPR Human)
   

   Use

   Lentiviral

   Tags

   Myc-DDK from backbone plasmid

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-CB-FLPe-P2A-copGFP-T2A-Puro

   Plasmid

   #72258

   Purpose

   Express FLPe, copGFP and Puromycin resistance gene under CB promoter

   Depositor

   Kazuhiro Oka

   Insert

   FLPe-P2A-copGFp-T2A-Puro
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CB and chicken beta-actin + CMV enhancer

   Available Since

   Jan. 15, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

   Plasmid

   #177434

   Purpose

   To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.

   Depositor

   David Andrews

   Insert

   Cb5 tail anchor (Cyb5r4 Rat)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

    Plasmid

    #138524

    Purpose

    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

    Depositor

    Richard Jenner

    Article

    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

    Insert

    PRC2-binding G-tract repeat RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-CAG-FLEX-NEUROD1-T2A-mCherry

    Plasmid

    #178584

    Purpose

    Cre-dependent expression of NEUROD1 and mCherry under the constitutive promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    NEUROD1 (NEUROD1 Human)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP

    Plasmid

    #75126

    Purpose

    Retroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domain

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    KMT6 (EZH2 Human)
    

    Use

    Retroviral

    Tags

    None

    Mutation

    SET domain replaced with the SET domain of EZH1

    Available Since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

13. nonphosphorylatable Mis12-targeted Aurora B FRET sensor

    Plasmid

    #45229

    Depositor

    Michael Lampson

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    non phosphorylatable Mis12-targeted Aurora B FRET sensor
    

    Expression

    Mammalian

    Mutation

    T-A mutation in substrate sequence

    Available Since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti CMV Puro DEST (w118-1) FAM149A

    Plasmid

    #107510

    Purpose

    Expressing FAM149a, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    FAM149A (FAM149A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)

    Plasmid

    #209084

    Purpose

    Ngn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expression

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    NGN2 (Neurog2 Mouse)
    BirA
    

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    hSyn

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)

    Plasmid

    #64314

    Purpose

    chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells

    Depositor

    Noriyuki Tsumaki

    Article

    Outani et al PLoS One. 2013 Oct 16;8(10):e77365. doi:

    Inserts

    COL11A2 promoter (COL11A2 Human)
    COL11A2 enhancer (COL11A2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP and IRES-PURO

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-BRD9_N216A-PGK-Puro-IRES-GFP

    Plasmid

    #75116

    Purpose

    Retroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutation

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Mutation

    single amino acid mutation N216A

    Available Since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

18. XLone-Puro Cas13d P2A eGFP-NLS

    Plasmid

    #155182

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d and nuclear eGFP

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    CasRx
    

    Use

    Unspecified; Piggybac transposon

    Tags

    NLS and P2A eGFP NLS

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-BRD9_BET-PGK-Puro-IRES-GFP

    Plasmid

    #75120

    Purpose

    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Mutation

    Bromodomain replaced with the first bromodomain o…

    Available Since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

20. Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G

    Plasmid

    #157984

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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