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pCMV-myc-LC3 (Atg8)

Plasmid

#24919

Depositor

Toren Finkel

Article

Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

Insert

LC3 (MAP1LC3B Human)

Tags

Myc

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pMax_ScaI_BFP

Plasmid

#177824

Purpose

Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

tagBFP

Expression

Mammalian

Mutation

5' inserted ScaI RE cut site

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLV.PGK.mFoxa2

Plasmid

#33014

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Forkhead box A2 (Foxa2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

H10L

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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yHS1-M7A

Plasmid

#159151

Purpose

Cytosolic labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

yHS1-M7A

Expression

Yeast

Mutation

Mutated Met 7 of the cyt b562 module of yHS1 to A…

Promoter

GPD

Available Since

Sept. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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His-MBP_G4G6 in pRSF

Plasmid

#188454

Purpose

Express in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

G4G6 (GSN Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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EXT1-pLJC2-3XFLAG

Plasmid

#215818

Purpose

Ectopic expression of EXT-1 in mammalian cells

Depositor

Yoav Shaul

Article

Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

Exostosin glycosyltransferase 1 (EXT1 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

Plasmid

#172067

Purpose

Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP11x7

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMito-LSSmKate1-N1

Plasmid

#31878

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

human cytochrome C oxidase subunit VIII (COX8A Human)

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pJR022

Plasmid

#198820

Purpose

C. elegans optimized version of mScarlet

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::wrmScarlet::let-858 3'UTR

Expression

Worm

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-EBNA2

Plasmid

#195815

Purpose

Gateway Entry Clone of Epstein-Barr virus EBNA2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

EBNA2 (EBNA-2 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX GFP T2A Cre

Plasmid

#63704

Purpose

Inducible expression of GFP T2A Cre fusion in mammalian cells

Depositor

Floris Foijer

Article

Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

T2A Cre

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR223-BLLF1(gp350)

Plasmid

#195837

Purpose

Gateway Entry Clone of Epstein-Barr virus BLLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BLLF1 (BLLF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK cyclin T1 (P#468)

Plasmid

#14607

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (CCNT1 Human)

Tags

GST

Expression

Bacterial

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
MAP4K3-GFP

Plasmid

#21731

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

Insert

MAP4K3 (MAP4K3 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

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Nurr1

Plasmid

#35000

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Nuclear receptor subfamily 4 group A member 2 (Nr4a2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 myc-Rab10

Plasmid

#208366

Purpose

Mammalian expression of myc-tagged human Rab10

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

myc-Rab10 (RAB10 Human)

Expression

Mammalian

Mutation

wild type

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hADARB2-3xFLAG

Plasmid

#165470

Purpose

Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Adenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hDDX41-HA

Plasmid

#165469

Purpose

Human DDX41 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

DEAD-box helicase 41 (DDX41 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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p6622 MSCV-P C HA GLTSCR

Plasmid

#34902

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

GLTSCR1 (BICRA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

July 20, 2012

Availability

Academic Institutions and Nonprofits only

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hGATA-1 WT

Plasmid

#118352

Purpose

This plasmid expresses human GATA-1 WT.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hGATA-1 (GATA1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

WT

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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