We narrowed to 2,346 results for: puc19

1. BC1450-H2B 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)

   Plasmid

   #164041

   Purpose

   CRISPR donor plasmid to insert mCherry-CRISPR-TagV2 into the C-terminus of human H2B gene

   Depositor

   Baohui Chen

   Article

   Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

   Insert

   mCherry-CRISPR-TagV2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

   Plasmid

   #181792

   Purpose

   Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)

   Plasmid

   #193048

   Purpose

   Heatshock expressed Cas9

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   hsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   hsp16.41p

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJBL7061

   Plasmid

   #167230

   Purpose

   cell-free cascaded lead sensor

   Depositor

   Julius Lucks

   Article

   Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

   Insert

   pPbr-AKSIRV
   

   Use

   Synthetic Biology

   Tags

   6X His

   Expression

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJBL7062

   Plasmid

   #167231

   Purpose

   cell-free cascaded copper sensor

   Depositor

   Julius Lucks

   Article

   Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

   Insert

   pCue-AKSIRV
   

   Use

   Synthetic Biology

   Tags

   6X His

   Expression

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLib6.4B-Aalb_743

   Plasmid

   #176676

   Purpose

   Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC017 - LwCas13a guide expression backbone with tRNAval promoter

   Plasmid

   #91907

   Purpose

   Backbone for cloning LwCas13a guides under tRNAval promoter

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a Guide site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD315 (mTurquoise2^SEC^2xHA)

   Plasmid

   #73343

   Purpose

   mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

   Insert

   mTurquoise2-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   2xHA and C. elegans codon-optimized mTurquoise2

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. ATP1A1_T804N_hPGK1_mScarlet-I_Donor

   Plasmid

   #173207

   Purpose

   HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I cassette to ATP1A1 intron 17.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1-T804N mScarlet-I donor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK1

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDT-sgRNA-XMAS-1x

    Plasmid

    #164413

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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