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pENTR221-TCF7

Plasmid

#79498

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

TCF7 (TCF7 Human)

Use

Gateway Cloning

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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TRE-mCherry-p2A-bReaches-TS

Plasmid

#158701

Purpose

Reporter construct

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

mCherry-p2A-bReaches-TS

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAct-PE2-HygroR

Plasmid

#149552

Purpose

Expression of PE2 enzyme under control of ubiquitous Actin5c promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Expression

Insect

Promoter

Act5c

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pHIP_RNA2

Plasmid

#61260

Purpose

Orsay virus RNA2 genome segment expression plasmid for virus reverse genetics

Depositor

David Wang

Article

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA2

Expression

Worm

Promoter

heat shock promoter

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pRVdG-4Halo3-mCherry

Plasmid

#59327

Purpose

Expresses eNpHR 3.0-mCherry

Depositor

Ian Wickersham

Insert

eNpHR 3.0-mCherry

Use

Rabies virus

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMAL-c2x-MT2

Plasmid

#32102

Depositor

David DeRosier, Chris Mercogliano, Cristina Risco Ortiz

Article

Mercogliano et al J Struct Biol. 2007 Oct;160(1):70-82. Ep

Insert

MT (2x) (Mt1 Mouse)

Tags

MBP

Expression

Bacterial

Promoter

P-lac

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only
Mef2D-shRNA

Plasmid

#32900

Depositor

Michael Greenberg

Article

Flavell et al Science. 2006 Feb 17;311(5763):1008-12.

Insert

Mef2d (Mef2d Rat)

Use

RNAi

Expression

Mammalian

Promoter

H1-RNA

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pSH824-ssr

Plasmid

#198027

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Apr resistance cassette

Expression

Bacterial

Promoter

AprR promoter

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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mNFAT4-462:TALE:VP64:KRɸ

Plasmid

#160662

Purpose

Constitutive expression of murine NFAT (aa 4-462) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

Depositor

Roman Jerala

Article

Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

Insert

mNFAT4-462:TALE:VP64:KRɸ

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAV0679

Plasmid

#149689

Purpose

Bacterial expression of SENP_EuH protease

Depositor

Dirk Görlich

Article

Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

Insert

SENP_EuH protease

Tags

His14-tag and Tev protease cleavage site

Expression

Bacterial

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pDN41

Plasmid

#61344

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-biNLS9 and PKIt NES

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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JpExpress404 PTEN

Plasmid

#49420

Purpose

Bacterial Expression Vector

Depositor

Ramon Parsons

Article

Hopkins et al Science. 2013 Jul 26;341(6144):399-402.

Insert

PTEN (PTEN )

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pSUPERretro-Sirt6 shRNA1

Plasmid

#53147

Purpose

shRNAi vector for Sirt6

Depositor

Katrin Chua

Article

Tennen et al Nat Commun. 2011 Aug 16;2:433. doi: 10.1

Insert

Sirt6

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

H1 RNA polymerase III

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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pUCBB-ntH6-eGFP

Plasmid

#32557

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Expression

Bacterial

Promoter

Consitutive lac

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas3

Plasmid

#178881

Purpose

Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas3c-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

Plasmid

#140000

Purpose

CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized CBE4max xCas9(3.7) with P2A-EGFP

Use

CRISPR

Tags

P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

Promoter

CAG

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pPAiRFP2-N1

Plasmid

#44273

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Nat Commun. 2013 Jul 10;4:2153. doi: 10.

Insert

PAiRFP2

Expression

Mammalian

Mutation

K69R/R83K/G120D/A123T/M163L/Q168E/R220P/S243N/V24…

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(WT)

Plasmid

#86561

Purpose

Expresses human full-length Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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px458_Conc3

Plasmid

#134450

Purpose

Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

Plasmid

#113039

Purpose

AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)

Use

AAV

Expression

Mammalian

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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