We narrowed to 55,786 results for: eng

1. pAAV-hsyn-GRAB_rDA-mut

   Plasmid

   #140558

   Purpose

   Expresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   Red fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-SUMO-mGSDMD

   Plasmid

   #111560

   Purpose

   Express mouse GSDMD in E.coli

   Depositor

   Hongbo Luo

   Article

   Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

   Insert

   Gasdermin D (Gsdmd Mouse)
   

   Tags

   His, SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSP1101

   Plasmid

   #65773

   Purpose

   Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, and T1337R mutations in C…

   Promoter

   CMV & T7

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ166-SpRY

   Plasmid

   #161520

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   zSpRYCas9
   

   Use

   CRISPR; Gateway compatible zsprycas9 entry clone

   Tags

   3X FLAG, NLS and NLS

   Expression

   Plant

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-GACh2.0

   Plasmid

   #121921

   Purpose

   express the genetically-encoded fluorescent acethycholine(ACh) sensor GACh2.0

   Depositor

   Yulong Li

   Article

   Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

   Insert

   GPCR activation based ACh sensor GACh2.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRE-mCardinal-P2A-B19L Flp-in vector

   Plasmid

   #178519

   Purpose

   For making TRE-CB mouse line in Tigre locus

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   mCardinal-P2A-B19L
   

   Expression

   Mammalian

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUT8-Dox

   Plasmid

   #124631

   Purpose

   Doxycycline-inducible FUT8 expression in mammalian cells

   Depositor

   Ron Weiss

   Article

   Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

   Inserts

   Alpha-(1,6)-fucosyltransferase
   Tetracycline repressor A3 mutant
   TagFP635
   

   Expression

   Mammalian

   Promoter

   TRE-tight and hEF1a

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ262B-ABE8e

   Plasmid

   #161524

   Purpose

   Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   TadA8e-zSpRYCas9(D10A)
   

   Use

   CRISPR; Gateway compatible tada8e-zsprycas9(d10a)…

   Expression

   Plant

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ266E

   Plasmid

   #161521

   Purpose

   Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   zSpRYCas9(D10A)-PmCDA1-UGI
   

   Use

   CRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA

    Plasmid

    #178518

    Purpose

    For making Rabies virus SAD B19 strain L cell line with hyPBase

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Insert

    B19L-IRES-mCherry
    

    Expression

    Mammalian

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PITX3-eGFP-PGK-Puro

    Plasmid

    #31942

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Insert

    PITX3 (PITX3 Human)
    

    Use

    Targeting donor

    Tags

    eGFP

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSUPER-retro-puro-shOrai1

    Plasmid

    #89933

    Purpose

    Knockdown human Orai1 expression

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    Orai1 shRNA (ORAI1 Human)
    

    Use

    Retroviral

    Available Since

    May 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDisplay-GACh1.0

    Plasmid

    #106072

    Purpose

    express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

    Insert

    GPCR activation based ACh sensor (GACh) 1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSUPER-retro-puro-shSTIM1

    Plasmid

    #89816

    Purpose

    Knockdown human STIM1 expression

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    STIM1 shRNA (STIM1 Human)
    

    Use

    Retroviral

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

    Plasmid

    #99229

    Purpose

    Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos2
    

    Tags

    Lifeact

    Expression

    Mammalian

    Mutation

    A69T for primed conversion applications

    Promoter

    CMV

    Available Since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSUPER-retro-puro-Smad4

    Plasmid

    #89829

    Purpose

    Knockdown human Smad4 expression in human mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

    Insert

    Smad4 shRNA (SMAD4 Mouse, Human)
    

    Use

    Retroviral

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET-pr-mKikGR1 (mKikGR1-V69T)

    Plasmid

    #99227

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mKikGR1
    

    Expression

    Bacterial

    Mutation

    monomeric variant with mutation V69T (Eos notatio…

    Promoter

    T7

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-mGSDMD1-251

    Plasmid

    #111565

    Purpose

    Express ELANE-cleaved mouse GSDMD in mammalian cells

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

19. B4GALT1-Dox

    Plasmid

    #124633

    Purpose

    Doxycycline-inducible B4GALT1 expression in mammalian cells

    Depositor

    Ron Weiss

    Article

    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

    Inserts

    Beta-(1,4)-Galactosyltransferase 1
    Tetracycline repressor A3 mutant
    TagFP635
    

    Expression

    Mammalian

    Promoter

    TRE-tight and hEF1a

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJS699

    Plasmid

    #168779

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD (S betacoronavirus)
    

    Tags

    Cmyc

    Mutation

    N343Q

    Promoter

    GAL1

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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