We narrowed to 3,302 results for: grna design
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Plasmid#236042PurposeThe plasmid pQdCas12a.sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene.DepositorInsertquorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
UseCRISPRPromoterquorum sensing promoterAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT451
Plasmid#139391PurposeBile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4)
UseSynthetic BiologyPromoterBA inducible promotersAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT449
Plasmid#139389PurposeBile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2)
UseSynthetic BiologyPromoterBile Acid inducible PromoterAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT448
Plasmid#139388PurposeBile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1),
UseSynthetic BiologyPromoterBile Acid inducible PromoterAvailable SinceJan. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT450
Plasmid#139390PurposeBile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR
UseSynthetic BiologyPromoterBile Acid inducible PromoterAvailable SinceJan. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT492
Plasmid#139397PurposeBile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5)
UseSynthetic BiologyPromoterBile Acid inducible PromoterAvailable SinceJan. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT493
Plasmid#139398PurposeBile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6)
UseSynthetic BiologyPromoterBile Acid inducible PromoterAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT494
Plasmid#139399PurposeBile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7)
UseSynthetic BiologyPromoterBile Acid inducible PromoterAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT455
Plasmid#139392PurposeaTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate), AmpR, ErmRDepositorInsertaTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate)
UseSynthetic BiologyPromoteraTC and BA inducible promotersAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
U6>XX
Plasmid#87542PurposeComponent 1 of 2 for U6>sgRNA cassette OSO-PCRDepositorTypeEmpty backboneAvailable SinceJan. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330 _HITI donor B6
Plasmid#172847PurposeDRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-nCas9n-nanos 3’UTR
Plasmid#62542Purposeexpression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafishDepositorAvailable SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHSN6A01
Plasmid#50586PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertsdCas9-VP64
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG, HA, and NLSMutationdCas9 = defective in DNA cleavage; 4×minimal VP16…Promoter2×35Sp and AtU6-26pAvailable SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBUN6A11
Plasmid#50579PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistanceDepositorInsertsdCas9-VP64
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG, HA, and NLSMutationdCas9 = defective in DNA cleavage; 4×minimal VP16…PromoterOsU3p and Ubi1pAvailable SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cas9/CD4-TK2
Plasmid#104397PurposeThe designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.DepositorAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human sgRNA library Brunello in lentiCRISPRv2
Pooled Library#73179PurposeHuman sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controlsDepositorHas ServiceLentiviral PrepExpressionMammalianUseCRISPR and LentiviralAvailable SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mouse sgRNA library Brie in lentiCRISPRv2
Pooled Library#73632PurposeMouse sgRNA library in backbone lentiCRISPRv2 containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controlsDepositorExpressionMammalianUseCRISPR and LentiviralAvailable SinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human sgRNA library Brunello in lentiGuide-Puro
Pooled Library#73178PurposeHuman sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controlsDepositorHas ServiceLentiviral PrepExpressionMammalianUseCRISPR and LentiviralAvailable SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mouse sgRNA library Brie in lentiGuide-Puro
Pooled Library#73633PurposeMouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controlsDepositorHas ServiceLentiviral PrepExpressionMammalianUseCRISPR and LentiviralAvailable SinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
p305N
Plasmid#246316PurposeA promoterless construct designed for cloning various Pol III promoters or serving as a control in evaluating Pol III promoters for CRISPR-Cas9 editing of mEGFP in Nicotiana benthamiana and poplar reporter linesDepositorInsertPromoterless
UseCRISPRExpressionPlantPromoterPromoterlessAvailable SinceOct. 30, 2025AvailabilityAcademic Institutions and Nonprofits only