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pEBTet_NAD-Snifit-nucl

Plasmid

#113915

Purpose

Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-p18-CIBN

Plasmid

#188990

Purpose

A lentiviral plasmid encoding lysosome-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

CIBN (CIB1 Mustard Weed)

Tags

p18

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-MSCV-4xS1m-GFP-T2A-PU

Plasmid

#138581

Purpose

For in vivo tagging

Depositor

Yin-Yuan Mo

Article

Hu et al Cell Death Differ. 2019 Dec 5. pii: 10.1

Type

Empty backbone

Use

Lentiviral

Tags

S1m tag

Promoter

MSCV

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2 8xSTAR-mScarletI-NLS. PGK-puro

Plasmid

#136263

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

mScarletI-NLS

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST ERKKTRrsFastLime

Plasmid

#205765

Purpose

Expression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

ERK KTR rsFastLime

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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Lv EF1a SS18-Frb PGK Puro

Plasmid

#102811

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express SS18-Frb fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

SS18-Frb

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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qTAG-C-sTagRFP-Puro

Plasmid

#207723

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Degron-KI donor backbone

Plasmid

#65483

Purpose

Donor backbone vector to clone left and right HR arms in for Degron-KI application

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Type

Empty backbone

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-miniTurbo

Plasmid

#207692

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miniTurbo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLentiCRISPR V2 sgIREB2_2

Plasmid

#169886

Purpose

Disrupt IREB2

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting IREB2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgATG7

Plasmid

#211534

Purpose

Deletes ATG7

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgATG7

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-EGFP:WTIP

Plasmid

#108231

Purpose

Lentiviral vector for Tet-inducible WTIP expression

Depositor

Kenneth Irvine

Article

Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

Insert

WTIP (WTIP Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Tet/ON Minimal CMV promoter

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 RagD 121L

Plasmid

#19318

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Q121L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
PLKO.1-SMG6-CDS-1

Plasmid

#136046

Purpose

SMG6 shRNA (Targeting CDS #1) inserted into the PLKO.1 plasmid (AACTTGTAAGTAACCTGCAGC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

SMG6 (SMG6 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-SMG6-CDS-2

Plasmid

#136047

Purpose

SMG6 shRNA (Targeting CDS #2) inserted into the PLKO.1 plasmid (AAGGAGTTCCAGGTGTTACTG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

SMG6 (SMG6 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-NELFE

Plasmid

#222559

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of NELFE

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

NELFE (NELFE Human)

Expression

Mammalian

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pEBTet_NADP-Snifit-nucl

Plasmid

#113918

Purpose

Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4

Plasmid

#192807

Purpose

Stable expression of 3xFLAG-NCOA4 in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_TARBP2

Plasmid

#106119

Purpose

Lentiviral expression of TARBP2

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TARBP2 (TARBP2 Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMA3288

Plasmid

#46885

Purpose

Lentiviral vector encoding Tet-inducible mitochondrially targeted Wild Type human Uracil-N-glycosylase

Depositor

Mikhail Alexeyev

Article

Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

Insert

Uracil-N-glycosylase WT (UNG Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

TRE-Tight

Available Since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

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