We narrowed to 16,615 results for: puromycin

1. pLKO.1 RagC shRNA #2

   Plasmid

   #26630

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   RagC shRNA (RRAGC Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pLJM1 RagA

   Plasmid

   #73031

   Purpose

   Expresses human RagA from a CMV promoter for stable expression in mammalian cells

   Depositor

   David Sabatini

   Article

   Efeyan et al Dev Cell. 2014 May 12;29(3):321-9. doi:

   Insert

   rragA (RRAGA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-N-Puro-miRFP670nano3

   Plasmid

   #207691

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miRFP670nano3 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pSMP-Luc

   Plasmid

   #36394

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   Firefly Luciferase
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pAc5 HA3-eDHFR-T2A-puro

   Plasmid

   #86395

   Purpose

   for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

   Depositor

   David Bentley

   Article

   Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

   Insert

   HA3 eDHFR T2A puro
   

   Use

   CRISPR

   Tags

   HA3 eDHFR T2A puro

   Mutation

   R12Y, G67S and Y100I mutations in E coli DHFR

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-CMV-tagRFP-Mad2

   Plasmid

   #114021

   Purpose

   Lentiviral delivery of tagRFP-MAD2 expressed from the CMV promoter

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   tagRFP-Mad2 (MAD2L1) (MAD2L1 Human)
   

   Use

   Lentiviral

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1 RagD shRNA #2

   Plasmid

   #30322

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   RagD shRNA (RRAGD Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   June 2, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pMRX-IP-3xFLAG-NCOA4 (dIDR2)

   Plasmid

   #192811

   Purpose

   Stable expression of 3xFLAH-NCOA4 (delta IDR2) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

   Insert

   NCOA4 (NCOA4 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Deletion of the IDR2

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMA3174

   Plasmid

   #46880

   Purpose

   Lentiviral vector for the dual control of expression through Tet-On promoter and C-terminal destabilizing domain

   Depositor

   Troy Stevens, Mikhail Alexeyev

   Article

   Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FFKBP12 E31G, R71G, K105E and myc

   Promoter

   TRE-Tight

   Available Since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. p663-UBC-miniTurbo-V5-NEK1_IDG-K

    Plasmid

    #189871

    Purpose

    Gateway destination clone of NEK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    NEK1 (NEK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBlueScript-humanAPOBEC1

    Plasmid

    #134606

    Purpose

    Plasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activity

    Depositor

    Silvestro Conticello

    Article

    Chieca et al bioRxiv 2021

    Insert

    humanAPOBEC1 (APOBEC1 Human)
    

    Expression

    Mammalian

    Promoter

    Beta actin

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRX-IP-3xFLAG-NCOA4 (dIDR1)

    Plasmid

    #192809

    Purpose

    Stable expression of 3xFLAH-NCOA4 (delta IDR1) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    NCOA4 (NCOA4 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deletion of the IDR1

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CAG.CenpB(FL)-GFP-Halo

    Plasmid

    #176205

    Purpose

    Centromeric anchor for TMP-Halo dimerization system

    Depositor

    Michael Lampson

    Article

    Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

    Insert

    CenpB-GFP-Halo
    

    Expression

    Mammalian

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p667-UBC-ADCK5-V5-miniTurbo_IDG-K

    Plasmid

    #189909

    Purpose

    Gateway destination clone of ADCK5 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ADCK5 (ADCK5 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p667-UBC-COQ8A-V5-miniTurbo_IDG-K

    Plasmid

    #189885

    Purpose

    Gateway destination clone of COQ8A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    COQ8A (COQ8A Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-PuroR Xlone eGFP

    Plasmid

    #179837

    Purpose

    Doxycycline-inducible expression of eGFP

    Depositor

    Xiaoping Bao

    Article

    Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

    Insert

    eGFP
    

    Use

    CRISPR, Synthetic Biology, and TALEN ; Donor plas…

    Expression

    Mammalian

    Promoter

    TRE3GS

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX302 LacZ-V5 puro

    Plasmid

    #98579

    Purpose

    Lentiviral vector for constitutive expression of human LacZ with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    LacZ
    

    Use

    Lentiviral

    Tags

    2x V5 tag

    Expression

    Mammalian

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPR - AAVS1 sgRNA

    Plasmid

    #70661

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against AAVS1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX-UBC_NKX2-1_Short_V5-GFP

    Plasmid

    #221499

    Purpose

    NKX2-1-GFP fusion protein lentiviral overexpression using an UBC promoter

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5-linker-GFP

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRAIP-SFB

    Plasmid

    #101769

    Purpose

    Mammalian expression of TRAIP fusion protein

    Depositor

    Michael Huen

    Article

    Feng et al Cell Discov. 2016 Jun 28;2:16016. doi: 1

    Insert

    TRAIP / RNF206 (TRAIP Human)
    

    Tags

    S peptide-flag-streptavidin binding peptide (SFB)

    Expression

    Mammalian

    Mutation

    siRNA-resistant allele

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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