We narrowed to 13,224 results for: CRISPR-Cas9

1. pX601-AAV-CMV::NLS-saCas9-NLS-3xHA-bGHpa;U6::BsaI-sgRNA-Grpr1

   Plasmid

   #175176

   Purpose

   A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Grpr

   Depositor

   Bernardo Sabatini

   Article

   Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

   Insert

   sgRNA-Grpr
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. iF67 PB-Zim3-XTEN-dCas9-mScarlet-Bsd-Neo-mAmetrine

   Plasmid

   #204732

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

   Plasmid

   #233371

   Purpose

   EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Sun et al bioRxiv 2024.09.11.611894

   Inserts

   NES-Kinprola_PKA-mEGFP
   FLAG-SV40NLS-Cas9-NLS-T2A-BSD
   

   Use

   Lentiviral

   Tags

   FLAG, NES, and mEGFP

   Expression

   Mutation

   Promoter

   EF1α

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

   Plasmid

   #84244

   Purpose

   Expresses GA-inducible VPR-Sa dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sa dCas9
   GID1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

   Expression

   Mammalian

   Mutation

   D10A, N580A and Synonymous mutation in PYL1 to re…

   Promoter

   CAG and PGK

   Available since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCas9-sgVRK1 #4-Blast

   Plasmid

   #199647

   Purpose

   Expresses Cas9 and sgRNA guide targeting VRK1

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A (VRK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCENPTΔC-dCas9-3xGFP

   Plasmid

   #198325

   Purpose

   Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus

   Depositor

   Sarah McClelland

   Article

   Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

   Insert

   CENPTΔC (CENPT Human)
   

   Use

   Lentiviral

   Tags

   EGFP x 3 and dCas9

   Expression

   Mammalian

   Mutation

   truncation - encodes only amino acids 1-375

   Promoter

   CMV-TetO

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiCas9-sgVRK1 #1-Blast

   Plasmid

   #199646

   Purpose

   Expresses Cas9 and sgRNA guide targeting VRK1

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A (VRK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiCas9-sgCh2-2-Blast

   Plasmid

   #199645

   Purpose

   Expresses Cas9 and sgRNA control guide targeting intergenic region

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9 (BB)-2A-GFP-RB1sg

   Plasmid

   #229857

   Purpose

   single guide RNA targeting human RB1 gene

   Depositor

   Maria Alvarado-Kristensson

   Article

   Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

   Insert

   RB1sgRNA (RB1 Human)
   

   Use

   Tags

   The gamma-tubulin sgRNA is coexpressed with 3xFLA…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-hUbC-GBX2-CDX124-Cas9-T2A-GFP

    Plasmid

    #192287

    Purpose

    Expresses Cas9-T2A-GFP and four sgRNAs: GBX2, CDX1, CDX2, CDX4

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    Cas9-T2A-GFP and four sgRNA: GBX2 + CDX1 + CDX2 + CDX4 (GBX2, CDX2, CDX1, CDX4 Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

    Plasmid

    #202555

    Purpose

    Synaptotagmin-1 sgRNA2 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid

    #202556

    Purpose

    Synaptotagmin-1 sgRNA3 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

    Plasmid

    #202554

    Purpose

    Synaptotagmin-1 sgRNA1 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. dCas9-CRY2Clust-FUSN-mut

    Plasmid

    #200970

    Purpose

    induction of phase-separated condensate mediated by FUSN-IDR mutant

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    FUSNmut
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    15 Tyr-to-Ser substitutions

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. px335-sgRNA-EF(Cas9wt)-Rif1

    Plasmid

    #122306

    Purpose

    Expresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Rif1 (Rif1 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV hUbC-Cas9-P2A-Puro_ST-sgRNA

    Plasmid

    #188705

    Purpose

    Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human safe targeting loci

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    ST sgRNAs
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9-2A-Puro-RAB7A-gRNA1 (PX459)

    Plasmid

    #221551

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

    Plasmid

    #167295

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    BAK (BAK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMC-Z-SpCas9-gRNA-repeat-polyT-11

    Plasmid

    #197781

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    gRNA repeat for U6 sgRNA assembly
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS

    Plasmid

    #121821

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-dCas9 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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