We narrowed to 26,884 results for: STI

1. pcDNA3.1_FLAG-YAP1

   Plasmid

   #173001

   Purpose

   constitutive expression of FLAG-tagged YAP1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   FLAG-YAP1 (YAP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

   Plasmid

   #208963

   Purpose

   Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   EcKlenow-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIRES proHB-EGF CS

   Plasmid

   #11603

   Depositor

   Rosalyn Adam

   Article

   Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

   Insert

   HB-EGF (HBEGF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Constitutively secreted HB-EGF: C-terminal amino …

   Promoter

   Available Since

   April 26, 2006

   Availability

   Academic Institutions and Nonprofits only

4. Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

   Plasmid

   #208046

   Purpose

   Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   RNF4 (RNF4 Human)
   

   Use

   Lentiviral

   Tags

   BirAopt

   Expression

   Mutation

   Promoter

   EFS

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Luciferase

   Plasmid

   #213979

   Purpose

   Expresses Doxy Inducible Luciferase

   Depositor

   Coleman Lindsley

   Article

   Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   6xHis affinity tag

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Tet-responsive promoter PTight, consisting of sev…

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLS13-IL-10

   Plasmid

   #209128

   Purpose

   Transient mammalian expression of IL-10

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   IL-10 (IL10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKM19

   Plasmid

   #123655

   Purpose

   Encodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cells

   Depositor

   Kevin Maringer

   Article

   Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Insect

   Mutation

   Promoter

   pIEx

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiDP1.3

   Plasmid

   #171096

   Purpose

   lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Cdt1 (AKA ROLECCS G1) and miniAID-fused mCherry2

   Depositor

   Dario Palmieri

   Article

   Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

   Insert

   OsTIR1-mEmerald-Cdt1-P2A-miniAID-mCherry2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Emerald, cdt1, miniAID, mCherry2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiDP1.2

   Plasmid

   #171095

   Purpose

   lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2

   Depositor

   Dario Palmieri

   Article

   Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

   Insert

   OsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Emerald, Geminin, miniAID, mCherry2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CD-MPR-mCherry-RUSH

    Plasmid

    #202798

    Purpose

    RUSH cargo: Str-KDEL_IRES_SBP-mCherry-CD-MPR

    Depositor

    Juan Bonifacino

    Article

    Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

    Insert

    M6PR (M6PR Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed 40 K (AAA) with R (AGA)

    Promoter

    CMV

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169

    Plasmid

    #178528

    Purpose

    Expresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    rtTA, SspB-mScarlet-I-MYPT (1-169aa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, Tet responsive element

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV7.1_3xFLAG-LATS1

    Plasmid

    #172986

    Purpose

    constitutive expression of FLAG-tagged LATS1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-LATS1 (LATS1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX304-MARK3

    Plasmid

    #107235

    Purpose

    Lentiviral gene expression vector for phospho-MARK3 expression

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MARK3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3 proHB-EGF CS

    Plasmid

    #11601

    Depositor

    Rosalyn Adam

    Article

    Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

    Insert

    HB-EGF (HBEGF Human)
    

    Use

    Tags

    His and myc

    Expression

    Mammalian

    Mutation

    Constitutively secreted HB-EGF: C-terminal amino …

    Promoter

    Available Since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pLenti6_YAP1-V5

    Plasmid

    #173002

    Purpose

    constitutive expression of V5-tagged YAP1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    YAP1-V5 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST

    Plasmid

    #208041

    Purpose

    Enables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    TurboID
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28b(+)_rPD-L1nb

    Plasmid

    #226715

    Purpose

    Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Recombinant PD-L1 nanobody
    

    Use

    Tags

    HA tag and Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV7.1_3xFLAG-WWC1

    Plasmid

    #172994

    Purpose

    constitutive expression of FLAG-tagged WWC1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-WWC1 (WWC1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-FLAG-Akt2-CA

    Plasmid

    #64050

    Purpose

    Myristoylated-FLAG-tagged Akt2, constitutively active

    Depositor

    Weiping Han

    Article

    Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

    Insert

    AKT2 (AKT2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and MYR

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSP2440

    Plasmid

    #72250

    Purpose

    Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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