We narrowed to 13,652 results for: nsf

1. pEHIShqprtR138Q

   Plasmid

   #92411

   Purpose

   hQPRTase harbouring R138Q mutation

   Depositor

   James Naismith, Huanting Liu

   Article

   Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

   Insert

   hQPRTase (QPRT Human)
   

   Use

   Tags

   TEV protease cleavable 6xHis

   Expression

   Bacterial

   Mutation

   R138Q

   Promoter

   T7

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEHIShqprtR102A

   Plasmid

   #92405

   Purpose

   hQPRTASE harbouring R102A mutation

   Depositor

   James Naismith, Huanting Liu

   Article

   Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

   Insert

   hQPRTase (QPRT Human)
   

   Use

   Tags

   TEV protease cleavable 6xHis

   Expression

   Bacterial

   Mutation

   R102A

   Promoter

   T7

   Available since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a - EtfB

   Plasmid

   #89617

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   electron-transfer-flavoprotein, alpha polypeptide
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. GST-PRMT9 deltaTPR mutant

   Plasmid

   #67614

   Purpose

   bacterial expression of GST-PRMT9 missing residues 21-139

   Depositor

   Steven Clarke

   Article

   Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

   Insert

   PRMT9 (PRMT9 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   truncation mutant missing aa 21-139

   Promoter

   tac

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

5. GST-PRMT9 deltaN (aa 21-350)

   Plasmid

   #67611

   Purpose

   bacterial expression of GST-PRMT9 missing residues 21-350

   Depositor

   Steven Clarke

   Article

   Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

   Insert

   PRMT9 (PRMT9 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   truncation mutant missing aa 21-350

   Promoter

   tac

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pJK591

   Plasmid

   #71589

   Purpose

   Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala/Asp177>Ala mutants (UctC-T2A,D177A)

   Depositor

   T. J. Kappock

   Article

   Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

   Insert

   acetyl-CoA:oxalate CoA-transferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes threonine-2 to alanine and aspartate-177 …

   Promoter

   T7

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV5-OCT-HA-eGFP

   Plasmid

   #67484

   Purpose

   Targets HA-eGFP to mitochondrial matrix

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   OCT-HA-eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   attenuated CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCMV3-OCT-HA-eGFP

   Plasmid

   #67482

   Purpose

   Targets HA-eGFP to mitochondrial matrix

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   OCT-HA-eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   attenuated CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pDEST14-SARA

   Plasmid

   #67389

   Purpose

   Expression of native hSara in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SARA (SAR1B Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET28b(+)-JHP933

    Plasmid

    #59302

    Depositor

    SeWon Suh

    Article

    Yoon et al Proteins. 2014 Mar 27. doi: 10.1002/prot

    Insert

    jhp0933
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    aa1-232

    Promoter

    T7

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pEBB-3XMyc-TRAF2 S408A

    Plasmid

    #44109

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 S408A (TRAF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Serine 408 to Alanine

    Promoter

    Available since

    May 2, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pWB-TRAF2 S102A

    Plasmid

    #44135

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 S102A (TRAF2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Serine 102 to Alanine

    Promoter

    Available since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pEBB-3XMyc-TRAF2 S102A

    Plasmid

    #44106

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 S102A (TRAF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Serine 102 to Alanine

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pLXSN-p85α-ΔiSH2-N

    Plasmid

    #39502

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    p85 ΔiSH2-N (PIK3R1 Bovine)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    ΔiSH2-N = deletion of AA478-513

    Promoter

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pMIG small T 88

    Plasmid

    #10691

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    SV40 small T Antigen 88 (SV40gp7 simian virus)
    

    Use

    Retroviral

    Tags

    IRES GFP

    Expression

    Mammalian

    Mutation

    contains only the N-termial 88 amino acids

    Promoter

    Available since

    Sept. 23, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pBMN(CMV-copGFP-Puro-SMAR)

    Plasmid

    #80391

    Purpose

    expression copGFP and Puromycine, with an S/MAR element for episomal maintaince

    Depositor

    Magnus Essand

    Article

    Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

    Insert

    copGFP-T2A-Puromycine
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2HA-pDEST-N

    Plasmid

    #118373

    Purpose

    Gateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-sgRNA-BSD

    Plasmid

    #175583

    Purpose

    Expresses sgRNA for CasRx in mammalian cell

    Depositor

    Qi Xie

    Article

    Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNuc-cis

    Plasmid

    #202796

    Purpose

    RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

    Depositor

    David Edgell

    Article

    Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

    Insert

    TevSpCas9, sgRNA cassette, oriT, CenArsHis
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pL1P4 ZmUbiP:GRF-GIF:NosT

    Plasmid

    #198048

    Purpose

    MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

    Depositor

    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

    Insert

    ZmUbiP::TaGRF4-GIF1::NosT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Zea mays (maize) ubiquitin promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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