We narrowed to 11,252 results for: NSI

1. MAC_C_CH60

   Plasmid

   #111662

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   CH60 (HSPD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMSCV-Flag-hKlf4

   Plasmid

   #20074

   Depositor

   Juan Belmonte

   Article

   Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

   Insert

   hKlf4 (KLF4 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40

   Plasmid

   #100846

   Purpose

   AAV expression of Cre recombinase-activated jRCaMP1a, a red fluorescent calcium sensor protein, from CAG promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1

   Insert

   jRCaMP1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV_OHu19986C_myc tag

   Plasmid

   #183174

   Purpose

   used to make AAV vectors for hsTRPA1 tagged with myc

   Depositor

   Sreekanth Chalasani

   Article

   Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

   Insert

   hsTRPA1 (TRPA1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSEMS-CV SUy-Halo7Tag

   Plasmid

   #108933

   Purpose

   Selflabeling tag at ATP Synthase

   Depositor

   Karin Busch

   Article

   Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

   Insert

   ATP synthase F1 subunit gamma (ATP5F1C Human)
   

   Use

   Tags

   Halo7 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. DPYD-FLAG

   Plasmid

   #61616

   Purpose

   The human DPYD was used to study the effect of DPYD expression on EMT

   Depositor

   David Sabatini

   Article

   Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

   Insert

   Dihydropyrimidine dehydrogenase (DPYD Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1_nV5-AMOT (p130)

   Plasmid

   #172997

   Purpose

   constitutive expression of V5-tagged AMOT (p130)

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   V5-AMOT (p130) (AMOT Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

   Plasmid

   #158953

   Purpose

   Express human RAD51(T309A) mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

   Insert

   RAD51 (RAD51 Human)
   

   Use

   Tags

   2X FLAG 2X STREP

   Expression

   Mammalian

   Mutation

   changed threonine 309 to alanine

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-hSyn-ADAR2-sesRNA-tTA2-W3SL

   Plasmid

   #192068

   Purpose

   Expression of ADAR2, sesRNA in mammalian cells with hSyn promoter, with tTA2 as efRNA and ADAR2 overexpression.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for mouse Ctip2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NSK021 CMV-TO-p53R248Q (FLP-IN)

    Plasmid

    #191167

    Purpose

    Expression of human p53 mutant R248Q in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    p53(R248Q) (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R248Q

    Promoter

    CMV-TO

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-Syn-ArchT-GFP

    Plasmid

    #63141

    Purpose

    AAV-mediated expression of ArchT-GFP under the Syn promoter. Using hGHpA signal.

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pXW117As(RS-E11-RinA)2

    Plasmid

    #123146

    Purpose

    Arsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J117, ParsR, PhrpLE, Pecf11

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FUG-T2A-Pten

    Plasmid

    #140738

    Purpose

    Lentiviral vector to overexpress human PTEN and GFP

    Depositor

    Bryan Luikart

    Article

    Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

    Insert

    GFP-PTA-PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    hUbiC

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-hsyn-GRAB_DA-mut

    Plasmid

    #140555

    Purpose

    Expresses the DA-insensitive control sensor GRAB_DA-mut in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    DA-insensitive sensor GRAB_DA-mut (control sensor)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax

    Plasmid

    #190153

    Purpose

    Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    Vamp2 (Vamp2 Mouse)
    

    Use

    AAV

    Tags

    P2AT2A-EGFP-caax

    Expression

    Mammalian

    Mutation

    Truncation that includes only AA #1-94

    Promoter

    MBP

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p420-3

    Plasmid

    #71257

    Purpose

    luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter

    Depositor

    Peter Cockerill

    Article

    Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.

    Insert

    3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    GM-CSF minimal

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

    Plasmid

    #158952

    Purpose

    Express human RAD51(F129A) mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    RAD51 (RAD51 Human)
    

    Use

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    changed phenylalanine 129 to alanine

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST40-2XFL-Src-S4-KD

    Plasmid

    #140297

    Purpose

    Expresses Flag-tagged human Src-S4-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    contains only the SH4 and KD regions

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1_nV5-AMOTL1

    Plasmid

    #172998

    Purpose

    constitutive expression of V5-tagged AMOTL1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    V5-AMOTL1 (AMOTL1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-4580NES

    Plasmid

    #138376

    Purpose

    Booster-JNK, a Red-Shifted Genetically Encoded FRET Biosensor for JNK activity.

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Booster-JNK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBp-FGFR2c-WT

    Plasmid

    #45699

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIc (FGFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40

    Plasmid

    #100849

    Purpose

    AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the CAG promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRCaMP1b
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-FLAG-MTOR-E1799K

    Plasmid

    #69009

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-E1799K (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Glutamate 1799 to Lysine

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-FLAG-MTOR-R2505P

    Plasmid

    #69015

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-R2505P (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Arginine 2505 to Proline

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EGFR D837A

    Plasmid

    #11014

    Purpose

    Retroviral construct for expressing human EGFR with D837A mutation in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR D837A (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    D837A

    Promoter

    Available since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

26. pDEST40-2XHA-Src-K298M

    Plasmid

    #140314

    Purpose

    Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    K298M

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40

    Plasmid

    #100850

    Purpose

    AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRCaMP1b
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SuExp-hPLD3

    Plasmid

    #201243

    Purpose

    express full length human PLD3 protein

    Depositor

    David Nemazee

    Article

    Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

    Insert

    PLD3 (PLD3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGL2-Mcl-1

    Plasmid

    #19132

    Depositor

    Wafik El-Deiry

    Article

    Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

    Insert

    Mcl-1 promoter (MCL1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMSCV-Flag-cMyc T58A

    Plasmid

    #20075

    Depositor

    Juan Belmonte

    Article

    Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

    Insert

    cMyc (MYC Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

31. pCAGGS-4591NES

    Plasmid

    #138377

    Purpose

    Booster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Booster-ROCK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV_hSyn1_AchLightG

    Plasmid

    #187464

    Purpose

    Expresses green acetylcholine indicator AchLightG in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    AchLightG
    

    Use

    AAV

    Tags

    Igκ leader

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

    Plasmid

    #216763

    Purpose

    PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLPC-FLAG.Mm.KAT5

    Plasmid

    #184647

    Purpose

    Retroviral expression of mouse Kat5

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    Kat5 (Kat5 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHH0103_HCLS1-1/1

    Plasmid

    #91437

    Purpose

    Protein expression and purification of human SH3 domain construct HCLS1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    HCLS1-1/1 (HCLS1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMVd1-EGFP-hRhoA-wt

    Plasmid

    #133926

    Purpose

    Low level expression of GFP fused human RhoA wild type

    Depositor

    Sebastián Dupraz, Frank Bradke

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    GFP-hRhoA WT (RHOA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. His-SUMO-Rad23A_pET11a

    Plasmid

    #169124

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hRad23A (RAD23A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-mitoExRai-AKAR2

    Plasmid

    #161757

    Purpose

    ExRai-AKAR2 targeted to the outer mitochondrial membrane.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    DAKAP-ExRai-AKAR2
    

    Use

    Tags

    N-terminal 30 amino acids from DAKAP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFBP_2xNbLYS1::LUZ

    Plasmid

    #189918

    Purpose

    Binary vector for Agrobacterium-mediated transient expression of a bioluminescent reporter for assessing immune activation in Nicotiana benthamiana by transiently expressed plant immune receptors.

    Depositor

    Adam Steinbrenner

    Article

    Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p2xNbLYS1:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Various

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-CAG-ArchT-tdTomato

    Plasmid

    #29778

    Purpose

    AAV-mediated expression of ArchT-tdTomato for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-tdTomato
    

    Use

    AAV; Adeno-associated virus

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge