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pCMV(Δ2)-squirrel-monkey-full-length-CHMP3-HA

Plasmid

#154170

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV(Δ1)-squirrel-monkey-full-length-CHMP3-HA

Plasmid

#154171

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-squirrel-monkey-full-length-CHMP3-HA

Plasmid

#154172

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-squirrel-monkey-CHMP3

Plasmid

#154182

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Tags

FLAG, Strep-Tag II, and preScission site

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-squirrel-monkey-retroCHMP3

Plasmid

#154184

Purpose

expresses squirrel monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Tags

FLAG, Strep-Tag II, and preScission site

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Gal4-TET1-878-911-I894A(pL84)

Plasmid

#124404

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with I894A mutation.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911(I894A mutation) (TET1 Human)

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

I894A

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-TET1-878-911-T898A(pL84)

Plasmid

#124403

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911(T898A mutation) (TET1 Human)

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

T898A

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-TET1-878-911-L900A(pL84)

Plasmid

#124402

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911(L900A mutation) (TET1 Human)

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

L900A

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-TET1-878-911-L897A(pL84)

Plasmid

#124401

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L897A mutation.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911(L897A mutation) (TET1 Human)

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

L897A

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-L900A(pL91)

Plasmid

#124395

Purpose

E.Coli produced TET1-SID mutants (L900A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-L900A (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

L900A

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-T898E(pL91)

Plasmid

#124394

Purpose

E.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-T898E (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

T898E

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-T898A(pL91)

Plasmid

#124393

Purpose

E.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-T898A (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

T898A

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-L897A(pL91)

Plasmid

#124392

Purpose

E.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-L897A (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

L897A

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-I894A(pL91)

Plasmid

#124391

Purpose

E.Coli produced TET1-SID mutants (I894A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-I894A (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

I894A

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-CenpB 1-166-dCEN-Incenp-GFP

Plasmid

#108486

Purpose

inducible expression of vsv-Mis12-dCEN-INCENP-EGFP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CenpB-dCEN-INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CEN box replaced by CenpB 1-166

Promoter

CMV

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-Incenp 8KQ

Plasmid

#108501

Purpose

expression of vsv-INCENP 8KQ

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

K548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, …

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-dCEN-Incenp

Plasmid

#108479

Purpose

expression of vsv-dCEN-INCENP

Depositor

Susanne Lens

Article

Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

Insert

dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box removed

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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HASP_HUMAN_D0

Plasmid

#79730

Purpose

This plasmid encodes the kinase domain of HASP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

HASP

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KCC2D_HUMAN_D0

Plasmid

#79734

Purpose

This plasmid encodes the kinase domain of KCC2D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2D

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
AAPK2_HUMAN_D0

Plasmid

#79738

Purpose

This plasmid encodes the kinase domain of AAPK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

AAPK2

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only