We narrowed to 18,569 results for: MUT

1. pCW-eGFP-FHA-SHLD2-m1

   Plasmid

   #114122

   Purpose

   Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatin

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD2 (m1 mutant) (SHLD2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   RNF8 FHA domain (aa 2-160) and eGFP

   Expression

   Mammalian

   Mutation

   W489A, F494A, W495A mutations introduced

   Promoter

   TRE promoter, Tet ON

   Available Since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAAV-CMV-PDGFRb559-562del-HA

   Plasmid

   #204351

   Purpose

   Expresses a mutant PDGFRb gene (559-562 deletion). Used for DNA delivery using Adeno-associated Virus.

   Depositor

   Hirofumi Nakatomi

   Article

   Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

   Insert

   Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
   

   Use

   AAV

   Tags

   HA

   Mutation

   559-562 deletion

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHIV-NAT-FLAG-CIP2A ∆NES (del561-625)

   Plasmid

   #222625

   Purpose

   Lentiviral vector that expresses Flag-tagged CIP2A NES mutant in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

   Insert

   CIP2A (CIP2A Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion of the residues 561-625 (ΔNES)

   Promoter

   EF-1-alpha promoter

   Available Since

   July 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pSYC-204

   Plasmid

   #178964

   Purpose

   Human GFAP (R79C) mutant fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombination

   Depositor

   Seok-Yong Choi

   Article

   Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1

   Inserts

   Glial fibrillary acidic protein promoter (gfap Zebrafish)
   Glial fibrillary acidic protein (GFAP Human)
   EGFP-pA
   

   Use

   Tol2 mediated recombination

   Tags

   3xFLAG

   Mutation

   R79C

   Promoter

   Zebrafish gfap promoter

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-EML4-ALK variant 3a I1171N

   Plasmid

   #183832

   Purpose

   Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N mutation in mammalian cells

   Depositor

   Aaron Hata

   Article

   Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-6a and ALK exon…

   Expression

   Mammalian

   Mutation

   I1171N

   Promoter

   CMV

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEH_AAV_TERT_prom_-124C>T_C7C_1.8kb

   Plasmid

   #183080

   Purpose

   rAAV transfer plasmid with ITRs flanking: (1) TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

   Depositor

   Aviv Regev

   Article

   Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

   Insert

   TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
   

   Use

   AAV; Homologous recombination donor template

   Mutation

   -124C>T promoter, Exon 1 C7C (C21>T21) sile…

   Promoter

   None for primary insert (Though partial TERT prom…

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb

   Plasmid

   #183081

   Purpose

   rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

   Depositor

   Aviv Regev

   Article

   Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

   Insert

   TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
   

   Use

   AAV; Homologous recombination donor template

   Mutation

   -146C>T promoter, Exon 1 C7C (C21>T21) sile…

   Promoter

   None for primary insert (Though partial TERT prom…

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS SARS-CoV-2 BA.2.75 Spike

   Plasmid

   #190012

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 BA.2.75 Spike

   Depositor

   Marceline Côté

   Article

   Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

   Insert

   SARS-CoV-2 BA.2.75 Spike (S )
   

   Expression

   Mammalian

   Mutation

   Contains the BA.2 mutations (T19I, LPPA24S, G142D…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available Since

   Sept. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pEH_AAV_TERT_prom_C7C_1.8kb

   Plasmid

   #183079

   Purpose

   rAAV transfer plasmid with ITRs flanking: (1) TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

   Depositor

   Aviv Regev

   Article

   Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

   Insert

   TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
   

   Use

   AAV; Homologous recombination donor template

   Mutation

   Wildtype promoter, Exon 1 C7C (C21>T21) silent…

   Promoter

   None for primary insert (Though partial TERT prom…

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-EML4-ALK variant 3a G1202R

    Plasmid

    #183830

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK G1202R mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Mutation

    G1202R

    Promoter

    CMV

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-EML4-ALK variant 3a L1196M/G1202R

    Plasmid

    #183831

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Mutation

    L1196M/G1202R

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-EML4-ALK variant 3a I1171N/D1203N

    Plasmid

    #183833

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Mutation

    I1171N/D1203N

    Promoter

    CMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKT-IDH1(R132H)-IRES-Katushka

    Plasmid

    #124257

    Purpose

    Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

    Insert

    Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)
    

    Expression

    Mammalian

    Mutation

    Arginine 132 is mutated to Histidine. This mutati…

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. SHP2(C459S)@pLKO-Trex-HA

    Plasmid

    #85459

    Purpose

    Expresses SHP2 mutant in mammalian cells

    Depositor

    Darrin Stuart

    Article

    Chen et al Nature. 2016 Jul 7;535(7610):148-52.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C459S

    Promoter

    RSV

    Available Since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

15. SHP2(T253M/Q257L)@pLKO-Trex-SBP

    Plasmid

    #85458

    Purpose

    SHP2 mutant gene expression

    Depositor

    Darrin Stuart

    Article

    Chen et al Nature. 2016 Jul 7;535(7610):148-52.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Lentiviral

    Tags

    SBP Tag

    Expression

    Mammalian

    Mutation

    T253M/Q257L

    Promoter

    RSV

    Available Since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. C321.ΔA

    Bacterial Strain

    #48998

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)

    Depositor

    George Church

    Article

    Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

    Bacterial Resistance

    Ampicillin

    Species

    Escherichia coli

    Available Since

    Oct. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pCSmEn2n4ERT(#234)

    Plasmid

    #184082

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    6his-myc-chkEn2(n4)-ERT2
    

    Use

    Synthetic Biology

    Tags

    6his-Myc

    Expression

    Mammalian

    Mutation

    chkEn2: G238S; ERT2: G400V, M543A, L544A, deleted…

    Promoter

    sCMV+SP6

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSmEn2SRERT(#231)

    Plasmid

    #184080

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    6his-myc-chkEn2(SR)-ERT2
    

    Use

    Synthetic Biology

    Tags

    6his-Myc

    Expression

    Mammalian

    Mutation

    chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …

    Promoter

    sCMV+SP6

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCSmEn25EERT(#232)

    Plasmid

    #184081

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    6his-myc-chkEn2(5E)-ERT2
    

    Use

    Synthetic Biology

    Tags

    6his-Myc

    Expression

    Mammalian

    Mutation

    chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

    Promoter

    sCMV+SP6

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. FGFR2 F276C 3xFlag

    Plasmid

    #110064

    Purpose

    FGFR2 F276C expression in mammalian cells (JCOPO paper)

    Depositor

    Martin Fernandez-Zapico

    Article

    Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

    Insert

    fibroblast growth factor receptor 2 (FGFR2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mutated Phenylalanine 276 to Cysteine (F276C)

    Promoter

    CMV

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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