166,818 results

1. TRE:GFP

   Plasmid

   #163036

   Purpose

   FLiCRE reporter gene for neuronal expression

   Depositor

   Alice Ting

   Article

   Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221_EGFP

   Plasmid

   #25899

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-iGlucoSnFR-TS

   Plasmid

   #127952

   Purpose

   Expresses the iGlucoSnFR-TS biosensor for intracellular [glucose], with a fluorescence-lifetime readout, in mammalian cells

   Depositor

   Gary Yellen

   Article

   Diaz-Garcia et al J Neurosci Res. 2019 May 20. doi: 10.100

   Insert

   iGlucoSnFR-TS
   

   Use

   AAV

   Tags

   His6 and Xpress epitope

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lamp1-RFP

   Plasmid

   #1817

   Purpose

   Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

   Depositor

   Walther Mothes

   Article

   Sherer et al Traffic 2003 Nov;4(11):785-801.

   Insert

   lysosome associated membrane protein 1 (Lamp1 Rat)
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   D50E (not important for function of plasmid)

   Promoter

   Available Since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_3xFlagEzh2 WT

   Plasmid

   #173717

   Purpose

   WT EZH2 expression plasmid with 3XFLAG tag

   Depositor

   Thomas Cech

   Article

   Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3_cAMPFIRE-M

   Plasmid

   #182280

   Purpose

   Mammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.

   Depositor

   Haining Zhong

   Article

   Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

   Insert

   cAMPFIRE-M (cAMP sensor)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 7TFP CDH1 reporter

   Plasmid

   #91704

   Purpose

   lentiviral luciferase reporter containing CDH1 promoter

   Depositor

   Bob Weinberg

   Article

   Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

   Insert

   CDH1 promoter (CDH1 Human)
   

   Use

   Lentiviral and Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CDH1

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP_sfCherry2(11)_H2B

   Plasmid

   #82605

   Purpose

   Expresses sfCherry2(11) tagged H2B in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(11)_H2B
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Gly12 to Ala in sfCherry11+

   Promoter

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJFRC19-13XLexAop2-IVS-myr::GFP

   Plasmid

   #26224

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   myr::GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMF440

    Plasmid

    #62550

    Purpose

    Broad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.

    Depositor

    Michael Franklin

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG1.1

    Plasmid

    #173685

    Purpose

    The expression vector for genes of interest under CAG promoter.

    Depositor

    Masahito Ikawa

    Article

    Ikawa et al J Biol Chem. 2011 Feb 18;286(7):5639-46.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-flex-taCasp3-TEVp (AAV1)

    Viral Prep

    #45580-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-flex-taCasp3-TEVp (#45580). In addition to the viral particles, you will also receive purified pAAV-flex-taCasp3-TEVp plasmid DNA. EF1a-driven, Cre-dependent, bicicstronic expression of designer pro-taCasp3 and TEVp. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Nirao Shah, Jim Wells

    Article

    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

    Promoter

    EF1a

    Tags

    None

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

13. Green Pegassos

    Plasmid

    #163114

    Purpose

    Expresses biosensors for pyruvate in mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

    Insert

    Green Pegassos
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-Traditional CAR (anti-CD19)

    Plasmid

    #192115

    Purpose

    Lentiviral delivery of Traditional CAR(anti-CD19)

    Depositor

    Wilson Wong

    Article

    Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

    Insert

    pHR-pEF1a-Traditional CAR (anti-CD19scFV_CD28_41BB_CD3z)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. ABE9

    Plasmid

    #194208

    Purpose

    Mammalian expression of ABE9

    Depositor

    Dali Li

    Article

    Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

    Insert

    TadA-8e (N108Q/L145T)-nSpCas9
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Bison sgRNA library

    Pooled Library

    #169942

    Purpose

    The BISON CRISPR library targets 713 E1, E2, and E3 ubiquitin ligases, deubiquitinases, and control genes and contains 2,852 guide RNAs. It was cloned into the pXPR003.

    Depositor

    Benjamin Ebert

    Article

    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

    Use

    Lentiviral

    Available Since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5FRT-EF-Pdgfralpha-EGFPN

    Plasmid

    #66787

    Purpose

    Expression of murine PDGFRalpha tagged with GFP under the control of EF1a promoter

    Depositor

    Lotte Pedersen

    Article

    Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

    Insert

    PDGFRalpha (Pdgfra Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

    Plasmid

    #190181

    Purpose

    Fluorescent reporter vector to visualize all of cell cycle phases.

    Depositor

    Toshiro Sato

    Article

    Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

    Inserts

    iRFP
    hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
    TagBFP2
    hGeminin(1/110)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-cT3G-cERP2-EGR1

    Plasmid

    #192913

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of EGR1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    EGR1 (EGR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRL092 pattB_cryaa:Venus_MCS

    Plasmid

    #213513

    Purpose

    attB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning

    Depositor

    Christian Mosimann

    Article

    Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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