We narrowed to 10,998 results for: ENA;

1. pCI.neo-rPAM1-M314I

   Plasmid

   #145802

   Purpose

   expresses catalytically inactive rat PAM-1 in mammalian cells

   Depositor

   Betty Eipper, Richard Mains

   Article

   Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.

   Insert

   rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed methionine 314 to isoleucine to inactivat…

   Promoter

   Available Since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas9GG

   Plasmid

   #131009

   Purpose

   Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Inserts

   direct repeat of SpCas9
   promoter PJ23119
   mRFP expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bba_R0040 TetR

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9FnCpf1GG

   Plasmid

   #131013

   Purpose

   Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Inserts

   direct repeat of SpCas9
   GFP expression cassette
   direct repeat of FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPD3cC

   Plasmid

   #86972

   Purpose

   Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

   Insert

   PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …

   Promoter

   T7 promoter

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-TripleColour-StAR

   Plasmid

   #222695

   Purpose

   Competition assay

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP, miRFP703, dTomato

   Expression

   Mammalian

   Mutation

   PGK, TagBFP, dTomato without BsmBI cutsite

   Promoter

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-mTET2

   Plasmid

   #89735

   Purpose

   Expresses wildtype mouse TET2 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   TET2 (Tet2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVTHM-shRnd3

   Plasmid

   #86437

   Purpose

   2nd generation doxycycline-inducible lentiviral vector expressing Rnd3-targeting shRNA and GFP

   Depositor

   Violaine Moreau

   Article

   Grise et al Hepatology. 2012 Jun;55(6):1766-75. doi:

   Insert

   shRNA targeting RND3 (RND3 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-UbC

   Plasmid

   #62806

   Purpose

   To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

   Depositor

   Jae Lee, Pantelis Tsoulfas

   Article

   Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

   Type

   Empty backbone

   Use

   AAV; Eucaryotic

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available Since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAP03-sp44/p21

   Plasmid

   #120232

   Purpose

   Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.

   Depositor

   Bradley Moore

   Article

   Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

   Insert

   bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   aac(3)IV (apramycin resistance gene)

   Promoter

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FLAG-mTET2 (N1041)

    Plasmid

    #89737

    Purpose

    Expresses the N1041 truncation to mouse TET2 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

    Insert

    TET2 (Tet2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N-term 1041 truncation of mouse TET2 (contains fi…

    Promoter

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPW3759 : CMVd3-HsIRE1deltaLD-HaloTag

    Plasmid

    #185681

    Purpose

    Low-level transient expression of delta-lumenal domain HsIRE1 with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Human, Synthetic)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Deletion of lumenal domain

    Promoter

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-FLAG-mTET2 (N500)

    Plasmid

    #89897

    Purpose

    Expresses mouse TET2 N500 truncation in mammalian cells

    Depositor

    Yue Xiong

    Article

    Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

    Insert

    TET2 (Tet2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N-term 500 truncation of mouse TET2 (contains fir…

    Promoter

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. DH5alpha::DddI

    Bacterial Strain

    #179631

    Purpose

    For expression of toxic DddA

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Bacterial Resistance

    Gentamicin

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

14. LeGO-iBSD-5LO-WT

    Plasmid

    #182741

    Purpose

    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (WT) in mamallian cells with blasticidin S resistance (bsd).

    Depositor

    Dieter Steinhilber

    Article

    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

    Insert

    5-Lipoxygenase (ALOX5 Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized for expression in homo sapiens

    Promoter

    SFFV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPtGE34

    Plasmid

    #107932

    Purpose

    Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

    Depositor

    David Edgell

    Article

    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

    Inserts

    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    

    Use

    CRISPR and Synthetic Biology; Episomal vector for…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-EF1a-extGluc-mKate-NGFR

    Plasmid

    #104831

    Purpose

    extGluc luciferase for invivo Imaging, mKate and NGFR for flourescent detection

    Depositor

    Irmela Jeremias

    Article

    Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

    Inserts

    mKate
    NGFR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-UbC-tdTomato

    Plasmid

    #62516

    Purpose

    Expresses tdTomato under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures

    Depositor

    Jae Lee, Pantelis Tsoulfas

    Article

    Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

    Insert

    tdTomato
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNK2657

    Plasmid

    #219741

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    hispidin- synthase from Mycena citricolor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-EF1a-extGluc-NGFR

    Plasmid

    #104832

    Purpose

    extGluc luciferase for invivo Imaging, NGFR for flourescent detection or separation

    Depositor

    Irmela Jeremias

    Article

    Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

    Inserts

    extGLuc
    NGFR
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LeGO-iBSD-5LO-d4

    Plasmid

    #182743

    Purpose

    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 4) in mamallian cells with blasticidin S resistance (bsd).

    Depositor

    Dieter Steinhilber

    Article

    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

    Insert

    5-Lipoxygenase isoform delta 4 (ALOX5 Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized for expression in homo sapiens

    Promoter

    SFFV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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