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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,448 results for: NEB

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Showing: 321 - 340 of 1448 results

  1. pGBW-m4092686

    Plasmid
    #153895
    Purpose
    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Expression
    Bacterial
    Mutation
    Nearly native encoding;inactivating mutations in …
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available Since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153895-plasmid-map-sequence-id-307318

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  2. pZH051

    Plasmid
    #47311
    Purpose
    Expresses Tsr-Venus-Ubiquitin-CI
    Depositor
    Jie Xiao
    Article
    Hensel et al Nat Struct Mol Biol. 2012 Aug;19(8):797-
    Insert
    wild type immunity region of lambda phage with tsr-venus-ub-cI
    Tags
    CI
    Expression
    Bacterial
    Promoter
    PR; PRM
    Available Since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pSPIH6

    Plasmid
    #190676
    Purpose
    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)
    Depositor
    John Vederas
    Article
    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi
    Inserts
    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    Tags
    Chitin binding domain, His tag, and N-terminal Hi…
    Expression
    Bacterial
    Mutation
    Added C-terminal his tag and None
    Promoter
    T7
    Available Since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    190676-plasmid-map-sequence-id-383624

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  14. pTXB1-H2B(1-114)_wt

    Plasmid
    #118252
    Purpose
    Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa
    Depositor
    Cynthia Wolberger
    Article
    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do
    Insert
    xlH2B(1-114)
    Tags
    Mxe-GyrA intein fused to chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    118252-plasmid-map-sequence-id-226720

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  17. pTXB1-xlH2B(1-114)_K114A

    Plasmid
    #118217
    Purpose
    Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.
    Depositor
    Cynthia Wolberger
    Article
    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do
    Insert
    xlH2B(1-114)
    Tags
    Mxe-GyrA intein fused to chitin binding domain
    Expression
    Bacterial
    Mutation
    Lysine 114 to alanine
    Promoter
    T7
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    118217-plasmid-map-sequence-id-226718

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Showing: 321 - 340 of 1448 results