We narrowed to 2,551 results for: PLS

1. pENTR-D-Topo-kcnk19/kcnk12l

   Plasmid

   #209001

   Purpose

   Riboprobe generation

   Depositor

   GuangJun Zhang

   Article

   Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

   Insert

   kcnk19 (kcnk12l Zebrafish)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-kcnk2b

   Plasmid

   #208984

   Purpose

   Riboprobe generation

   Depositor

   GuangJun Zhang

   Article

   Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

   Insert

   kcnk2b (kcnk2b Zebrafish)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pENTR-D-Topo-kcnk4a

   Plasmid

   #208987

   Purpose

   Riboprobe generation

   Depositor

   GuangJun Zhang

   Article

   Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

   Insert

   kcnk4a (kcnk4a Zebrafish)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Cdk12_intron3_sg2_pX330

   Plasmid

   #127174

   Purpose

   sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cdk12 Intron 3 sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   U6 Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cdk12_intron4_sg1_pX458

   Plasmid

   #127175

   Purpose

   sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cdk12 Intron 4 sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   U6 Promoter

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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6. Galphai1-mTurquoise2

   Plasmid

   #69620

   Purpose

   The heterotrimeric G-protein subunit, Gαi isoform 1, tagged with mTurquoise2

   Depositor

   Dorus Gadella

   Article

   van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

   Insert

   Galphai1-mTurquoise2
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

7. Galphai2-mTurquoise2

   Plasmid

   #69621

   Purpose

   The heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2

   Depositor

   Dorus Gadella

   Article

   van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

   Insert

   Galphai2-mTurquoise2
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Galphai3-mTurquoise2

   Plasmid

   #69622

   Purpose

   The heterotrimeric G-protein subunit, Gαi isoform 3, tagged with mTurquoise2

   Depositor

   Dorus Gadella

   Article

   van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

   Insert

   Galphai3-mTurquoise2
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCali2

   Plasmid

   #86119

   Purpose

   Multiplex temperature-gated expression of mWasabi and mCherry reporters

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA36
   mWasabi
   TcI
   mCherry
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T and D341A, P60L, D135V, K187R, K202I, L208Q

   Promoter

   pR/pL, pR/pL, pLacI, pTlpA, and pTlpA, pLacI

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FRB-VMAc-CSarcin

    Plasmid

    #70223

    Purpose

    Expresses VMA intein with C-terminal portion of alpha-sarcin

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (C-terminal portion)
    

    Use

    Tags

    FKBP-rapamycin binding (FRB) domain of the mammal…

    Expression

    Mammalian

    Mutation

    Changed Asp 28 to Cys, deleted amino acids 1-27

    Promoter

    CMV

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

11. 3XFLAG-NSarQ27G-VMAn-FKBP

    Plasmid

    #70229

    Purpose

    Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (N-terminal portion)
    

    Use

    Tags

    3XFLAG, FK506- and rapamycin-binding protein (FKB…

    Expression

    Mammalian

    Mutation

    Changed Gln 27 to Gly

    Promoter

    CMV

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-FRB-VMAc-CGFP

    Plasmid

    #70226

    Purpose

    Expresses C-terminal portion of GFP along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    eGFP
    

    Use

    Tags

    FKBP-rapamycin binding (FRB) domain of the mammal…

    Expression

    Mammalian

    Mutation

    Changed Ile 129 to Cys, Deleted amino acids 1-128

    Promoter

    CMV

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pDONR221-kcnk18

    Plasmid

    #209000

    Purpose

    Riboprobe generation

    Depositor

    GuangJun Zhang

    Article

    Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

    Insert

    kcnk18 (kcnk18 Zebrafish)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTcI38-Wasabi

    Plasmid

    #86117

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI38
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G

    Promoter

    pR/pL and pR/pL, LacI

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW57.1-pDUXCca

    Plasmid

    #198236

    Purpose

    Lentivector with tet-inducible porcine DUXC (codon altered)

    Depositor

    Stephen Tapscott

    Article

    Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

    Insert

    porcine DUXC (codon altered)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon altered

    Promoter

    TRE

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. dFP-mini

    Plasmid

    #120543

    Purpose

    Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.

    Depositor

    Brian Chow

    Article

    Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

    Insert

    de novo fluorescence-activating protein mini (dFP-mini)
    

    Use

    Tags

    6xHis - TEV cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBEAST_J23101-amidase

    Plasmid

    #128135

    Purpose

    The cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    amidase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221-kcnk3a

    Plasmid

    #208985

    Purpose

    Riboprobe generation

    Depositor

    GuangJun Zhang

    Article

    Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

    Insert

    kcnk3a (kcnk3a Zebrafish)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEBTet-SNAP-HCA_sensor

    Plasmid

    #127182

    Purpose

    Eukaryotic expression of the fusion protein used for sulfa-drugs sensing

    Depositor

    Kai Johnsson

    Article

    Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

    Insert

    SNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA
    

    Use

    Tags

    HisTag and StrepTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEBTet-p52-HDM2_sensor

    Plasmid

    #127184

    Purpose

    Eukaryotic expression of the fusion protein used for drug diruption the p53-HDM2 interaction

    Depositor

    Kai Johnsson

    Article

    Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

    Insert

    p53-YPeT-Pro15-GGS2-Pro15-CFP-HDM2
    

    Use

    Tags

    HisTag and StrepTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge