Purpose
In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.
Insert
hCas9 with C. elegans 5' and 3' UTRs
Available Since
Sept. 13, 2013
Availability
Academic Institutions and Nonprofits only