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Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease

Plasmid kit

#1000000087

Purpose

A plasmid collection, based on the Tol2kit, used to streamline the production of transgenic zebrafish for studying neurodegenerative disorders.

Depositor

Nicholas Cole

Application

Cloning and Synthetic Biology

Vector type

Zebrafish Expression

Cloning type

MultiSite Gateway

Available since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only
pGTQL1211YN

Plasmid

#61704

Purpose

Gateway compatible BiFC vector (pEarleyGate201-YN)

Depositor

Yuhai Cui

Publication

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Use

Bifc assay

Tags

YFP-N and HA

Expression

Plant

Promoter

35S

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pGTQL1221YC

Plasmid

#61705

Purpose

Gateway compatible BiFC vector (pEarleyGate202-YC)

Depositor

Yuhai Cui

Publication

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Use

Bifc assay

Tags

YFP-C and FLAG

Expression

Plant

Promoter

35S

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA5FRT/Flag-OGT1

Plasmid

#29760

Depositor

Joan Conaway

Publication

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

OGT1 (OGT Human)

Tags

Flag

Expression

Mammalian

Mutation

N764D and K1010E

Available since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pRK5-HA-YFP-rictor

Plasmid

#73387

Purpose

Expresses HA and YFP tagged Rictor

Depositor

Jie Chen, Taekjip Ha

Publication

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

RPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)

Tags

HA and YFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pU6-Cj-sgRNA

Plasmid

#89753

Purpose

U6 promoter driven expression of Cj-SgRNA cloned with BsmbI

Depositor

Seokjoong Kim

Publication

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

Cj-SgRNA

Use

Sgrna expression under u6 promoter

Promoter

U6

Available since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcdna nesprin HL

Plasmid

#68128

Purpose

nesprin headless control

Depositor

Daniel Conway

Publication

Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

Insert

nesprin-2 (Syne2 Mouse)

Expression

Mammalian

Mutation

deleted actin-binding domain

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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MAC-tag-N

Plasmid

#108078

Purpose

Gateway compatible destination vectors containing StrepIII/HA/BioID

Depositor

Markku Varjosalo

Publication

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CCDB (ccdB(letD) )

Tags

StrepIII-HA-BirA*

Expression

Bacterial and Mammalian

Available since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pDMyc-TNPO1

Plasmid

#86081

Purpose

A mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Transportin-1 (TNPO1 Human)

Tags

Myc

Expression

Mammalian

Promoter

T7

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-TIP49b

Plasmid

#15358

Depositor

Joan Conaway, Ronald Conaway

Publication

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

RuvB-like 2 (RUVBL2 Human)

Tags

FLAG

Expression

Mammalian

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MAC-tag-C

Plasmid

#108077

Purpose

Gateway compatible destination vectors containing StrepIII/HA/BioID

Depositor

Markku Varjosalo

Publication

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CCDB (ccdB(letD) )

Tags

StrepIII-HA-BirA*

Expression

Bacterial and Mammalian

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pFGAMS-EGFP

Plasmid

#99107

Purpose

Expresses human PFAS-EGFP fusion in mammalian cells

Depositor

Stephen Benkovic

Publication

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

PFAS (PFAS Human)

Tags

EGFP (enhanced green fluorescent protein)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMX-Brg1

Plasmid

#25855

Depositor

Hans Schöler

Publication

Singhal et al Cell. 2010 Jun 11. 141(6):943-55.

Insert

Brahma related gene 1 (Smarca4 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pSR59.4

Plasmid

#63175

Purpose

Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

Depositor

Jeffrey Tabor

Publication

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

OmpR (ompR )
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pMDC32-HPB

Plasmid

#32078

Depositor

Fumi Katagiri

Publication

Qi et al Plant J. 2009 Mar;57(5):932-44. Epub 200

Type

Empty backbone

Use

Gateway destination binary vector

Tags

HA-Prescission-Biotin

Promoter

35S promoter

Available since

Dec. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEGFP PICH (Nigg CB62)

Plasmid

#41163

Depositor

Erich Nigg

Publication

Baumann et al Cell. 2007 Jan 12;128(1):101-14.

Insert

PICH (ERCC6L Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pSF3-Flag-CBir-FRB_Myc-NBir-FKBP

Plasmid

#90003

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Publication

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

NBirA*-linker-FKBP
CBirA*-linker-FRB

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX_DSB_Spectrum-V1

Plasmid

#192474

Purpose

Lentiviral DSB Spectrum V1 Reporter System

Depositor

Michael Yaffe

Publication

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, incompleteGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLS-mP

Plasmid

#81225

Purpose

Lentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.

Depositor

Nadav Ahituv

Publication

Inoue et al Genome Res. 2016 Nov 9. pii: gr.212092.1

Type

Empty backbone

Use

Lentiviral

Promoter

Derived from pGL4.23

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCAG-BirA*-dCas9-eGFP

Plasmid

#84779

Purpose

Expresses BirA*-dCas9-eGFP in mammalian cells.

Depositor

Heinrich Leonhardt

Publication

Schmidtmann et al Nucleus. 2016 Sep 27:0.

Insert

BirA*-dCas9-eGFP

Use

CRISPR

Tags

cMyc

Expression

Bacterial and Mammalian

Promoter

CAG

Available since

July 29, 2017

Availability

Academic Institutions and Nonprofits only

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