We narrowed to 3,495 results for: ttl

1. pJRTCLCrVA009

   Plasmid

   #22842

   Depositor

   Niki Robertson

   Article

   Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

   Insert

   A DNA containing CHLI fragment
   

   Use

   Cotton vigs vector

   Expression

   Bacterial

   Mutation

   Cotton Leaf Crumple Virus Coat Protein (AR1 gene)…

   Available Since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB9

   Plasmid

   #210036

   Purpose

   Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas9
   puro-T2A-eGFP
   

   Use

   CRISPR

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-Miro1 (184-618)

   Plasmid

   #127626

   Purpose

   Human Miro1 lacking the first GTPase domain

   Depositor

   Josef Kittler

   Article

   Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

   Insert

   Miro1 (RHOT1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   184-618 only

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYL192 (TRV1)

   Plasmid

   #148968

   Purpose

   Tobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

   Insert

   TRV RNA1
   

   Use

   Plant vector with trv rna1; this vector used with…

   Mutation

   P697S mutation in putative replicase. S239G and S…

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-dCas13-M3nls

   Plasmid

   #155366

   Purpose

   Targeted m6A RNA methylation in mammalian cells

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGMβ1

   Plasmid

   #216202

   Purpose

   Chromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.

   Depositor

   Yasuko Sasaki

   Article

   Iwamoto et al Biosci Microbiota Food Health. 2022;41(1

   Type

   Empty backbone

   Use

   Shuttle vector, conjugal plasmid, theta type rep…

   Expression

   Bacterial

   Promoter

   lac promoter in pGEM-Teasy,　Promoters in pAMbeta1…

   Available Since

   Jan. 21, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. CYFIP1.mCherry

   Plasmid

   #122051

   Purpose

   Expresses human CYFIP1 in mammalian cells.

   Depositor

   Josef Kittler

   Article

   Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

   Insert

   CYFIP1 (CYFIP1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPDK3876(TRV-RNA2-pPEBV-MCS)

   Plasmid

   #149275

   Purpose

   Modified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAs

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter
   

   Use

   T-dna vector

   Mutation

   T2318C (DNA) in PEBV coat protein sequence

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST47-CYFIP1.GFP

   Plasmid

   #109139

   Purpose

   GFP-tagged CYFIP1 expression construct

   Depositor

   Josef Kittler

   Article

   Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

   Insert

   CYFIP1 (CYFIP1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB1163

    Plasmid

    #210032

    Purpose

    Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas11-P2A-Cas6-T2A-Cas3
    rtta-T2A-blast
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDNR-SpCas9-Cdt1

    Plasmid

    #80426

    Purpose

    Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Cdt1
    

    Use

    CRISPR

    Tags

    Cdt1

    Mutation

    codon optimized for human

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. myc-KIF5A

    Plasmid

    #127616

    Purpose

    N-terminal myc-tagged mouse KIF5A

    Depositor

    Josef Kittler

    Article

    Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

    Insert

    KIF5A (Kif5a Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST47-CYFIP2.GFP

    Plasmid

    #109140

    Purpose

    GFP-tagged CYFIP2 expression construct

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP2 (CYFIP2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLNCX1 myr Akt1 delta4-129

    Plasmid

    #15989

    Depositor

    Morris Birnbaum

    Article

    Tuttle et al Nat Med. 2001 Oct . 7(10):1133-7.

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Retroviral

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Delta 4-129

    Available Since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

15. myc-AP4 Epsilon (V454X)

    Plasmid

    #127624

    Purpose

    N-terminal myc-tagged AP4 epsilon subunit (V454X)

    Depositor

    Josef Kittler

    Article

    Ivankovic et al Autophagy. 2019 May 29:1-17. doi: 10.108

    Insert

    AP-4 complex subunit epsilon-1 (AP4E1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Premature stop codon (V454X)

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB9v2

    Plasmid

    #210029

    Purpose

    Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB9P2

    Plasmid

    #210030

    Purpose

    Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    prime editor 2
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPB758

    Plasmid

    #210033

    Purpose

    Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas7-P2A-Cas5-T2A-Cas8
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-Miro1 (1-177 fused to 562-618)

    Plasmid

    #127627

    Purpose

    First GTPase domain of human Miro1 fused to C-terminus

    Depositor

    Josef Kittler

    Article

    Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

    Insert

    Miro1 (RHOT1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    1-177 fused to 562-618

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-Miro1 (412-618)

    Plasmid

    #127628

    Purpose

    GTPase2 domain of human Miro1 including transmembrane domain and C-terminal amino acids

    Depositor

    Josef Kittler

    Article

    Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

    Insert

    Miro1 (RHOT1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    412-618 only

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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