We narrowed to 3,808 results for: mak

1. pCsB_spacer

   Plasmid

   #136076

   Purpose

   Use when making odd level constructs (L3,...) if pCsB position (position 2) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Even spacer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pCsA_spacer

   Plasmid

   #136075

   Purpose

   Use when making odd level constructs (L3,...) if pCsA position (position 1) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Even spacer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. PB-HD252

   Plasmid

   #172656

   Purpose

   Expressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 gene

   Depositor

   Jay Shendure

   Article

   Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   pegRNA-HD252A/pegRNA-HD252B
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p3E-APEX2 (GC rich)

   Plasmid

   #115550

   Purpose

   Multisite gateway vector for 3' tagging with GC-rich APEX2. Used to make 115563. Parton lab clone HHS

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   APEX2 (GC rich)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIVT/Acrosin-GFP-GPI

   Plasmid

   #122141

   Purpose

   For making acrosin-GFP-GPI RNA

   Depositor

   Carmen Williams

   Article

   Winuthayanon et al Elife. 2015 Dec 1;4:e10453. doi: 10.7554

   Insert

   acrosin-GFP-GPI
   

   Use

   For in vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MSCV-6NBC-GFP-PGK-Puro-Barcode9

   Plasmid

   #83686

   Purpose

   individual 6N barcode to make barcoded cell line variants

   Depositor

   Monte Winslow

   Article

   Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

   Insert

   Barcode9
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   please view depositor comments below

   Promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MSCV-6NBC-GFP-PGK-Puro-Barcode7

   Plasmid

   #83684

   Purpose

   individual 6N barcode to make barcoded cell line variants

   Depositor

   Monte Winslow

   Article

   Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

   Insert

   Barcode7
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   L119P in GFP

   Promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-PRB

   Plasmid

   #89130

   Purpose

   mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418

   Depositor

   Elizabeth Wilson

   Article

   Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

   Insert

   human progesterone receptor-B (PGR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV-Syn1-mCherry

   Plasmid

   #222967

   Purpose

   Make lentivirus to express mCherry in syn1 expressing cells

   Depositor

   Xinyu Zhao

   Article

   Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   synapsin I

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTipi2.1_Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa

    Plasmid

    #221356

    Purpose

    Mammalian expression of Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa; to be used with Anti-Strep [IPI-C23.21] heavy chain (Plasmid 221355) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa

    Plasmid

    #221360

    Purpose

    Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa; to be used with Anti-V5 [IPI-SV5-Pk1] heavy chain (Plasmid 221359) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pYPQ132B2.0

    Plasmid

    #99888

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU3

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQ131A2.0

    Plasmid

    #99884

    Purpose

    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYPQ132A2.0

    Plasmid

    #99887

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ133A2.0

    Plasmid

    #99891

    Purpose

    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBMN(CMV-copGFP-Luc2-Puro)

    Plasmid

    #80389

    Purpose

    To make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection marker

    Depositor

    Magnus Essand

    Article

    Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

    Insert

    copGFP-T2A-Luc2-F2A-Puromycin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTipi2.1_Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit HC

    Plasmid

    #221363

    Purpose

    Mammalian expression of Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit HC; to be used with Anti-His [IPI-HAP1/5.13.22] light chain (Plasmid 221364) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pTipi2.1_Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa

    Plasmid

    #221364

    Purpose

    Mammalian expression of Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa; to be used with Anti-His [IPI-HAP1/5.13.22] heavy chain (Plasmid 221363) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. modRNAc0-Cas9-2A-GFP

    Plasmid

    #170181

    Purpose

    IVT template plasmid for making modified Cas9GFP RNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    Cas9-2A-GFP
    

    Use

    Ivt template plasmid

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIVT-p53DD

    Plasmid

    #232751

    Purpose

    IVT template for making modRNA encoding p53DD, a mutant p53 that lacks DNA binding ability

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    p53DD
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    deletion of DNA binding domain

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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