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C413-E22: attB4-polyhedrinp>-attB1r

Plasmid

#162922

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pIhh-5A9-LUC

Plasmid

#46318

Depositor

Xiangli Yang

Article

Wang et al Development. 2009 Dec;136(24):4143-53. d

Insert

5 copies of A9 sequence from Ihh promoter

Tags

luciferase

Expression

Mammalian

Promoter

mouse osteocalcin gene 2 promoter

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pYES2.1-cerMET5

Plasmid

#107437

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET5 (MET5 Budding Yeast)

Tags

V5-His

Expression

Yeast

Mutation

V81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …

Promoter

GAL1

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSopBC460S

Plasmid

#112514

Purpose

PsopB driven sopB (catalytically inactive) and sigE

Depositor

Olivia Steele-Mortimer

Article

Finn et al PLoS Pathog. 2017 Apr 20;13(4):e1006354.

Insert

sopB (catalytically inactive)/sigE operon

Expression

Bacterial

Mutation

SopB C460S

Promoter

PsopB

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-PIM2

Plasmid

#51848

Purpose

promoter region (-550;0) of PIM2

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

PIM2 (PIM2 Human)

Use

Luciferase; Reporter vector

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-NBR1(ΔUBD)

Plasmid

#119691

Purpose

For mammalian expression of NBR1 without its UBD

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

NBR1 (NBR1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

UBD deletion (1-906)

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pBL119-2

Plasmid

#104336

Purpose

Expresses thsR from a TetR regulated promoter under ATC control. Expresses sfGFP from a thsR regulated promoter. Expresses mCherry from a constituative promoter.

Depositor

Jeffrey Tabor

Article

Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

Insert

Thiosulfate response regulator

Expression

Bacterial

Promoter

PLTetO1

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pKC123

Plasmid

#134240

Purpose

expresses gRNA targeting CENP A 3'

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

CENP A 3' CRISPR construct

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2

Plasmid

#128167

Purpose

Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, GSAx9 linker)

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2

Use

Cre/Lox

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMDC43-SEIPIN3

Plasmid

#96978

Purpose

Express GFP-tagged Arabidopsis SEIPIN3 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN3 (AT2G34380 Mustard Weed)

Tags

GFP

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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roGFP_iL_pQE30

Plasmid

#48633

Purpose

Bacterial expression vector encoding for the 6XHis tagged redox probe roGFPiL (roGFP C147, L147b, C204)

Depositor

David Ron

Article

Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

Insert

roGFP1

Tags

HisTag

Mutation

insertion L147B

Available Since

Oct. 8, 2013

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D1 C7G HA

Plasmid

#8954

Depositor

Philip Hinds

Article

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D1 (CCND1 Human)

Tags

HA

Expression

Mammalian

Mutation

C7G: point mutation in LxCxE motif, LxghE

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pmiReport PU.1 3' UTR

Plasmid

#26530

Depositor

David Baltimore

Article

O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

Insert

PU.1 3' UTR (Spi1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
plentiCRISPR-sgMDH1

Plasmid

#72875

Purpose

The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgMDH1

Use

Lentiviral

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
Rc/CMV cyclin D1 LxCxE HA

Plasmid

#8956

Depositor

Philip Hinds

Article

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D1 (CCND1 Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of LxCxE motif

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pMDC43-SEIPIN2

Plasmid

#96977

Purpose

Express GFP-tagged Arabidopsis SEIPIN2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN2 (AT1G29760 Mustard Weed)

Tags

GFP

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMDC43-SEIPIN1

Plasmid

#96976

Purpose

Express GFP-tagged Arabidopsis SEIPIN1 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Tags

GFP

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBW216J106-Amp32C

Plasmid

#78651

Purpose

pSB3K3 carrying J106-30hrpR-32hrpS-t-hrpL-30gfp-tT

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J106-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pGL-RbUTR

Plasmid

#20883

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

Rb 3'UTR (Rb1 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
pMDC84-SEIPIN1

Plasmid

#96979

Purpose

Express GFP-tagged Arabidopsis SEIPIN1 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Tags

6xHis and GFP

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGL-Lats2UTR

Plasmid

#20882

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

Lats2 3'UTR (Lats2 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
pMH-HT_ygiF-FL

Plasmid

#127795

Purpose

Expresses full-length ygiF in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

ygiF (ygiF Escherichia coli)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBW214J115-Amp32C

Plasmid

#78649

Purpose

pSB3K3 carrying J115-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J115-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-taiman

Plasmid

#17584

Depositor

Denise Montell

Article

Bai et al Cell. 2000 Dec 22. 103(7):1047-58.

Insert

taiman (tai Fly)

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
As5 (pBW414J105-arsR)

Plasmid

#123162

Purpose

Arsenic sensor used in the sensor cell array. pSB3K3 carrying J105-30arsR-t-ParsR-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J105-30arsR-t-ParsR-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J105, ParsR

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSiP4

Plasmid

#21960

Depositor

Klaus Rajewsky

Article

Xiao et al Nat Immunol. 2008 Apr . 9(4):405-14.

Insert

Pten (Pten Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pBW211J109-Amp32C

Plasmid

#78646

Purpose

pSB3K3 carrying J109-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J109-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pSiB3

Plasmid

#21958

Depositor

Klaus Rajewsky

Article

Xiao et al Nat Immunol. 2008 Apr . 9(4):405-14.

Insert

BCL2L11 (Bcl2l11 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pGL3-PIM2mut1

Plasmid

#51849

Purpose

promoter region of PIM2 lacking the forkhead binding site #2

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

PIM2 (PIM2 Human)

Use

Luciferase; Reporter vector

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
Rc/CMV cyclin D2 LxCxE HA

Plasmid

#8957

Depositor

Philip Hinds

Article

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D2 (CCND2 Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of LxCxE motif

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pBW213J116-Amp32C

Plasmid

#78648

Purpose

pSB3K3 carrying J116-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J116-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
Rc/CMV cyclin D2 K112E HA

Plasmid

#8953

Depositor

Philip Hinds

Article

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D2 (CCND2 Human)

Tags

HA

Expression

Mammalian

Mutation

K112E: point mutation in cyclin box

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pNH002

Plasmid

#42145

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

N-TAP TAG::[(G4S)3]::mTFP1

Tags

TAP TAG

Expression

Worm

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pNH026

Plasmid

#42148

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1::linker::TAP TAG

Tags

TAP-Tag

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pNH001

Plasmid

#42144

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

TAP-tag

Tags

TAP-tag

Expression

Worm

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
PsiB1

Plasmid

#21959

Depositor

Klaus Rajewsky

Article

Xiao et al Nat Immunol. 2008 Apr . 9(4):405-14.

Insert

BCL2L11 (Bcl2l11 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pCaSpeR-hs-taiman

Plasmid

#17585

Depositor

Denise Montell

Article

Bai et al Cell. 2000 Dec 22. 103(7):1047-58.

Insert

taiman (tai Fly)

Expression

Insect

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
pLNHA-C1-HsNot8_J

Plasmid

#146699

Purpose

Mammalian Expression of HsNot8

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot8 (CNOT8 Human)

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ gcsf

Plasmid

#132947

Purpose

Expresses zebrafish granulocyte colony stimulating factor (csf3a)

Depositor

Jason Berman

Article

Rajan et al Leukemia. 2021 Sep 30. pii: 10.1038/s413

Insert

gcsf (csf3a Zebrafish)

Use

Mrna synthesis

Promoter

SP6

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C453-E18: attB4-polyhedrinp>-attB5

Plasmid

#162977

Purpose

Gateway attB4/attB5 entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV polyhedrin promoter

Use

Synthetic Biology

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 6801 - 6840 of 12807 results

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