We narrowed to 13,613 results for: crispr cas9

1. px335-sgRNA-EF(Cas9n)-Rad21-L

   Plasmid

   #122313

   Purpose

   Expresses sgRNA targeting mouse Rad21 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Rad21
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. px335-sgRNA-EF(Cas9n)-Rad21-R

   Plasmid

   #122314

   Purpose

   Expresses sgRNA targeting mouse Rad21 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Rad21
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. px335-sgRNA-EF(Cas9n)-Ssrp1-L

   Plasmid

   #122315

   Purpose

   Expresses sgRNA targeting mouse Ssrp1 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Ssrp1
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. px335-sgRNA-EF(Cas9n)-Ssrp1-R

   Plasmid

   #122316

   Purpose

   Expresses sgRNA targeting mouse Ssrp1 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Ssrp1
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

   Plasmid

   #167296

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   BAX (BAX Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

   Plasmid

   #107857

   Purpose

   To knock-out TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   gRNA against TARDBP (Exon 1) (TARDBP Human)
   

   Expression

   Mammalian

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-Nvec-codonopti-Cas9-2xNLS

   Plasmid

   #141108

   Purpose

   Plasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensis

   Depositor

   Matthew Gibson

   Article

   Hill et al Methods Mol Biol. 2022;2450:437-465. doi

   Insert

   Nematostella codon optimized Cas9
   

   Use

   Unspecified

   Mutation

   All amino acids coded for by codons optimized for…

   Promoter

   T7

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9

   Plasmid

   #107858

   Purpose

   To knock-out TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   gRNA against TARDBP (Exon 2) (TARDBP Human)
   

   Expression

   Mammalian

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-L3-Csy4-FokI-dCas9-EGFP-L2

   Plasmid

   #141047

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Csy4-2A-FokI-dCas9-2A-EGFP
   

   Expression

   Mammalian

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid

    #217798

    Purpose

    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR and Lentiviral

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    EF1a

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX601-AAV-CMV::NLS-saCas9-NLS-3xHA-bGHpa;U6::BsaI-sgRNA-lacZ

    Plasmid

    #175175

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting lacZ

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    sgRNA-lacZ
    

    Use

    AAV and CRISPR

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX601-AAV-CMV::NLS-saCas9-NLS-3xHA-bGHpa;U6::BsaI-sgRNA-Grpr1

    Plasmid

    #175176

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Grpr

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    sgRNA-Grpr
    

    Use

    AAV and CRISPR

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. iF67 PB-Zim3-XTEN-dCas9-mScarlet-Bsd-Neo-mAmetrine

    Plasmid

    #204732

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid

    #84244

    Purpose

    Expresses GA-inducible VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sa dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

    Expression

    Mammalian

    Mutation

    D10A, N580A and Synonymous mutation in PYL1 to re…

    Promoter

    CAG and PGK

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPRv2-7SK-Blast

    Plasmid

    #183620

    Purpose

    lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.

    Depositor

    Bill Sugden

    Article

    Hutcheson et al ACS Synth Biol. 2022 Jun 29. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Modified 7SK

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCENPTΔC-dCas9-3xGFP

    Plasmid

    #198325

    Purpose

    Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    CENPTΔC (CENPT Human)
    

    Use

    Lentiviral

    Tags

    EGFP x 3 and dCas9

    Expression

    Mammalian

    Mutation

    truncation - encodes only amino acids 1-375

    Promoter

    CMV-TetO

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiCas9-sgVRK1 #4-Blast

    Plasmid

    #199647

    Purpose

    Expresses Cas9 and sgRNA guide targeting VRK1

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A (VRK1 Human)
    

    Use

    Lentiviral

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiCas9-sgVRK1 #1-Blast

    Plasmid

    #199646

    Purpose

    Expresses Cas9 and sgRNA guide targeting VRK1

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A (VRK1 Human)
    

    Use

    Lentiviral

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiCas9-sgCh2-2-Blast

    Plasmid

    #199645

    Purpose

    Expresses Cas9 and sgRNA control guide targeting intergenic region

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A
    

    Use

    Lentiviral

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9 (BB)-2A-GFP-RB1sg

    Plasmid

    #229857

    Purpose

    single guide RNA targeting human RB1 gene

    Depositor

    Maria Alvarado-Kristensson

    Article

    Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

    Insert

    RB1sgRNA (RB1 Human)
    

    Tags

    The gamma-tubulin sgRNA is coexpressed with 3xFLA…

    Expression

    Mammalian

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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