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YCe1693 HC_Kan_RFP-p1

Plasmid

#100609

Purpose

EMMA toolkit part-entry vector position 1

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1500 HC_Kan_RFP-p2

Plasmid

#100610

Purpose

EMMA toolkit part-entry vector position 2

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1502 HC_Kan_RFP-p3

Plasmid

#100611

Purpose

EMMA toolkit part-entry vector position 3

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1505 HC_Kan_RFP-p4

Plasmid

#100612

Purpose

EMMA toolkit part-entry vector position 4

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1506 HC_Kan_RFP-p5

Plasmid

#100613

Purpose

EMMA toolkit part-entry vector position 5

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR207-Citrine-PTS1

Plasmid

#44588

Purpose

Gateway entry vector for Citrine fluorescent protein with a peroxisome localization signal

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-PTS1

Tags

PTS1 (peroxisomal targeting signal type 1)

Available Since

Sept. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a-KstR

Plasmid

#51179

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

KstR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET21a-CampR

Plasmid

#51162

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

CampR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pGST1-yVps27-UIM1

Plasmid

#44426

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

Vps27-UIM1 (VPS27 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
C119

Plasmid

#174354

Purpose

CRISPR-Cas9 with guide targeting human PABPC1 genomic location near STOP codon

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

gRNA sequence targeting human PABPC1 near stop codon region

Use

CRISPR

Availability

Academic Institutions and Nonprofits only

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C205

Plasmid

#174359

Purpose

For expressing siRNA-resistant frog ePAB in human cell lines via transfection

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

ePAB

Tags

FLAG

Expression

Mammalian

Mutation

Multiple silent mutations introduced to be resist…

Availability

Academic Institutions and Nonprofits only

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p6XHis-T7(P266L)

Plasmid

#174866

Purpose

Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

Depositor

Anna Pyle

Article

Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

Insert

T7 RNA polymerase

Tags

6XHis-tag

Expression

Bacterial

Mutation

P266L

Promoter

lac promoter

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pET28:GFP

Plasmid

#60733

Purpose

pET28 driving expression of GFP, biobrick part Bba_E0040

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pSMT3-M

Plasmid

#26589

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

n/a

Use

Shuttle vector for mycobacteria

Available Since

May 6, 2013

Availability

Academic Institutions and Nonprofits only

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pCLX-pTF-R1-DEST-R2-EBR65

Plasmid

#45952

Depositor

Patrick Salmon

Article

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pLNCX2-Scrib

Plasmid

#24600

Depositor

Senthil Muthuswamy

Article

Zhan et al Cell. 2008 Nov 28. 135(5):865-78.

Insert

Scribble (SCRIB Human)

Expression

Mammalian

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pMSCV PIG (Puro IRES GFP empty vector)

Plasmid

#21654

Purpose

Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pHCMV_GP64

Plasmid

#207276

Purpose

Mammalian expression of baculovirus GP64 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

GP64

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMF19

Plasmid

#113662

Purpose

Containing the wbbL (rhamnosyltransferase) gene cloned for mutant complementation

Depositor

Miguel Valvano

Article

Feldman et al J Biol Chem. 1999 Dec 3;274(49):35129-38

Insert

wbbL

Promoter

Ptac

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 mouse PARG (439-959)

Plasmid

#132615

Purpose

expresses mouse PARG(439-959) in E. coli.

Depositor

Wenqing Xu

Article

Wang et al PLoS One. 2014 Jan 21;9(1):e86010. doi:

Insert

PARG (439-959)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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