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tUI

Plasmid

#122661

Purpose

Express tUI protein in E. coli

Depositor

Robert Cohen

Article

Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

tUI (UBQLN1, USP5 Human)

Tags

His-tag

Promoter

T7 promoter

Available Since

July 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCas-CmR(+)

Plasmid

#113633

Purpose

Chloramphenicol resistant derivative of pCas plasmid

Depositor

Olivier Lichtarge

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

Chloramphenicol resistance gene

Use

CRISPR

Promoter

Pcat

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag-RagC(S75N)

Plasmid

#99724

Purpose

Mammalian expression of Flag-RagC(S75N)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Tags

Flag

Mutation

S75N

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag-RagA(Q66L)

Plasmid

#99716

Purpose

Mammalian expression of Flag-RagA(Q66L)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Tags

Flag

Mutation

Q66L

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Lysosomes-20

Plasmid

#58022

Purpose

Localization: Lysosome Membrane, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

LAMP1

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PLlacO-1-mfLon

Plasmid

#45152

Depositor

Michel Maharbiz

Article

Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Insert

Lon

Use

Synthetic Biology

Tags

ssRA degradation (LVA)

Expression

Bacterial

Promoter

PLlacO-1

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pFU-GW-sMYH6-mNeon-TNNT2-mScarlet

Plasmid

#170712

Purpose

dual fluorescent reporter for MYH6 (short) and TNNT2 promoters

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Inserts

mNeonGreen
mScarlet

Use

Lentiviral

Expression

Mammalian

Promoter

TNNT2 and aMHC

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMT643

Plasmid

#8610

Depositor

Robert Hartley

Article

Hartley et al Biochemistry 1993 Jun 15;32(23):5978-84.

Insert

barstar A

Expression

Bacterial

Mutation

Two cysteines of barstar changed to alanine, C40A…

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only

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FLM-PC1

Plasmid

#41567

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

sequence from 4078 - 4303 of PKD1

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-CSNK1G3

Plasmid

#23400

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSNK1G3 (CSNK1G3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-PIK3C3

Plasmid

#23457

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIK3C3 (PIK3C3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PI4K2B

Plasmid

#23507

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PI4K2B (PI4K2B Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDonor_e-attP-H1-g3-Ef1a-Puro-P2A-mCherry

Plasmid

#247160

Purpose

Engineered e-attP donor plasmid expressing puromycin resistance and mCherry with donor binding sgRNA H1-g3

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Puromycin resistance, mCherry

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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LifeAct-mhYFP

Plasmid

#186524

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to actin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

LifeAct

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pKP3915

Plasmid

#113335

Purpose

Beta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Insert

Artificial Transcription Factor (McIsaac et al, 2013)

Expression

Yeast

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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USP19 (C506A)-FLAG/pRK5

Plasmid

#36307

Depositor

Xiaolu Yang

Article

Mei et al J Biol Chem. 2011 Oct 14;286(41):35380-7

Insert

ubiquitin specific peptidase 19 (USP19 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C506A

Promoter

CMV

Available Since

May 11, 2012

Availability

Academic Institutions and Nonprofits only
pBABE Hygro ST6GalNaC5

Plasmid

#26981

Depositor

Joan Massague

Article

Bos et al Nature. 2009 Jun 18. 459(7249):1005-9.

Insert

ST6GalNaC5 (ST6GALNAC5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-MAST2

Plasmid

#23456

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAST2 (MAST2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TAOK3

Plasmid

#23861

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TAOK3 (TAOK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pFastBac1-Ls-AChBP

Plasmid

#63126

Purpose

Expression of Lymnaea stagnalis AChBP

Depositor

Titia Sixma

Article

Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

Insert

AChBP

Expression

Insect

Promoter

Polyhedrin

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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