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pDONR223-MINK1

Plasmid

#23522

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MINK1 (MINK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCAG-rtTA

Plasmid

#163601

Purpose

Expresses rtTA in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

rtTA

Expression

Mammalian

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti-dCas9-TurboID-blast

Plasmid

#222599

Purpose

Express fussed dCas9-TurboID for proximity labeling

Depositor

Yarui Diao

Publication

Sun et al Nat Genet. 2023 Aug;55(8):1324-1335. doi

Insert

dCas9-TurboID

Use

Lentiviral

Promoter

EF-1α

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR221-NR1H2

Plasmid

#79513

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H2 CDS

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

LXR BETA (NR1H2 Human)

Use

Gateway Cloning

Promoter

no Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-ACVR2B

Plasmid

#23401

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ACVR2B (ACVR2B Human)

Use

Gateway Cloning

Mutation

A333G, 1MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-LATS2

Plasmid

#23485

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LATS2 (LATS2 Human)

Use

Gateway Cloning

Mutation

aa182-762 deleted

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
SAP130

Plasmid

#11241

Depositor

Robin Reed

Publication

Das et al Mol Cell Biol. 1999 Oct . 19(10):6796-80

Insert

SAP130 (SF3B3 Human)

Expression

Bacterial

Available since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMV-Flag YAP S381A

Plasmid

#27377

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Tags

2xFLAG

Expression

Mammalian

Mutation

S381A

Available since

March 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pDONR223-BMPR1B

Plasmid

#23638

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BMPR1B (BMPR1B Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
MSCV-CMV-DsRed-IRES-d24EGFP

Plasmid

#41944

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Publication

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV.CAG-FLEX.SF-iGluSnFR.A184S

Plasmid

#106186

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

CAG

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Lentihair s1GFP

Plasmid

#8451

Depositor

Bob Weinberg

Publication

Stewart et al RNA 2003 Apr;9(4):493-501.

Insert

siRNA against GFP

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pOSCAR

Plasmid

#29639

Depositor

David_L Andrews, Scott Gold

Publication

Paz et al Fungal Genet Biol. 2011 Jul;48(7):677-84

Type

Empty backbone

Available since

Sept. 19, 2011

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pGH020_sgRNA_G418-GFP

Plasmid

#85405

Purpose

hu6 driven sgRNA vector with G418 and GFP selectable markers

Depositor

Michael Bassik

Publication

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

G418 resistance and GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLminP_Luc2P_RE53

Plasmid

#90398

Purpose

STAT1 - STAT1 gene reporter

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

STAT1

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSMALB-ATF4.12

Plasmid

#155033

Purpose

Fluorescent reporter for ATF4 translation, Negative Control

Depositor

John Dick, Peter van Galen

Publication

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

uORF1 start codon mutated

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe 3XFLAG-wtGATA6-3XAU1 puro

Plasmid

#72607

Purpose

Expresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cells

Depositor

Kevin Janes

Publication

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-FHC-POLQ-DM

Plasmid

#64880

Purpose

lentiviral expression of human POLQ double mutant (in POL and HDL domains)

Depositor

Richard Wood

Publication

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

K121M and D2330A,Y2331A

Promoter

EF1alpha

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3A

Plasmid

#50854

Purpose

expresses constituvely active cofilin in mammalian cells

Depositor

James Bamburg

Publication

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Expression

Mammalian

Mutation

constitutively active, S3A

Promoter

CMV

Available since

Feb. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles
pET26-MBP-GLIC

Plasmid

#20887

Depositor

Raimund Dutzler

Publication

Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

Insert

pentameric ligand gated ion channel from Gloeobacter violaceus PCC 7421

Tags

His, MBP, and pelB

Expression

Bacterial

Available since

Oct. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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