We narrowed to 15,924 results for: aga

1. pET11+PCNA3-CYP119

   Plasmid

   #66136

   Purpose

   Expresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

   Insert

   CYP119 (cyp119 )
   

   Use

   Tags

   Sulfolobus solfataricus PCNA3

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET11+PCNA3-CYP119(T214V)

   Plasmid

   #66137

   Purpose

   Expresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

   Insert

   CYP119 (cyp119 )
   

   Use

   Tags

   Sulfolobus solfataricus PCNA3

   Expression

   Bacterial

   Mutation

   T214V

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b+PCNA3 T180C

   Plasmid

   #66181

   Purpose

   Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 3
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   T180C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15b+PCNA3 R112C

   Plasmid

   #66180

   Purpose

   Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 3
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b+PCNA2 E105C

   Plasmid

   #66176

   Purpose

   Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 2
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E105C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET11+PCNA3-CYP119(D77R/T214V)

   Plasmid

   #66138

   Purpose

   Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

   Insert

   CYP119 (cyp119 )
   

   Use

   Tags

   Sulfolobus solfataricus PCNA3

   Expression

   Bacterial

   Mutation

   D77R, T214V

   Promoter

   T7 promoter

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pASKt15C+SrtA5woH

   Plasmid

   #65019

   Purpose

   Expresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

   Insert

   sortase A
   

   Use

   Tags

   S tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, P94R, D160N, D165A, K19…

   Promoter

   tetracycline promoter/operator

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTH1-SaSrtA-KQ

   Plasmid

   #64982

   Purpose

   Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E105K, E108Q

   Promoter

   Available since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nano-lantern(Ca2+)_63/pRSETB

   Plasmid

   #51980

   Purpose

   bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM_E104Q-4GS
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pattB-3xFlag-Loqs-PA

    Plasmid

    #41108

    Depositor

    Phillip Zamore

    Article

    Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

    Insert

    Loquacious-PA (loqs Fly)
    

    Use

    Tags

    3xFlag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pSpCas9(BB)-2A-Puro (PX459) V2.0

    Plasmid

    #62988

    Purpose

    Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9-2A-Puro V2.0
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57.1-Tet-UPF1WT (pRKB264)

    Plasmid

    #99146

    Purpose

    Inducible lentiviral construct to express FLAG-tagged UPF1wt

    Depositor

    Robert Bradley

    Article

    Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    First 127 codons are optimized to reduce GC conte…

    Promoter

    pTight

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. STARR-seq luciferase validation vector_ORI_empty

    Plasmid

    #99297

    Purpose

    Luciferase validation vector; empty vector without insert to determine baseline

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMK292 (mAID-mCherry2-NeoR)

    Plasmid

    #72830

    Purpose

    C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mCherry2-Neo
    

    Use

    CRISPR

    Tags

    mAID-mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDrVSP-IRES2-EGFP

    Plasmid

    #80333

    Purpose

    Expresses Danio rerio VSP with separated EGFP in mammalian cell

    Depositor

    Yasushi Okamura

    Article

    Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

    Insert

    Daino rerio voltage-sensing phosphatase (tpte Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCVFLAG-ZFP423

    Plasmid

    #24764

    Depositor

    Bruce Spiegelman

    Article

    Gupta et al Nature. 2010 Mar 25;464(7288):619-23. do

    Insert

    Zinc Finger Protein 423 (Zfp423 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pQCXIP-BSR-GFP11

    Plasmid

    #68716

    Purpose

    Split GFP assay

    Depositor

    Yutaka Hata

    Article

    Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

    Insert

    bsr
    

    Use

    Retroviral

    Tags

    FLAG and GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-JunWT-Myc

    Plasmid

    #47443

    Purpose

    mammalian expression of WT c-Jun

    Depositor

    Axel Behrens

    Article

    Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

    Insert

    Jun (Jun Mouse)
    

    Use

    Tags

    Flag and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. HDAC6 Flag

    Plasmid

    #13823

    Purpose

    Mammalian expression of human histone deacetylase 6 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HDAC7 Flag

    Plasmid

    #13824

    Purpose

    Mammalian expression of human histone deacetylase 7 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC7 (HDAC7 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge