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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,346 results for: multi

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Showing: 3461 - 3480 of 18346 results

  2. Gβ-2A-cpV-Gγ2-IRES-Gαi2-mTq2

    Plasmid
    #69624
    Purpose
    A FRET sensor for Galphai2 activation, encoded on a single plasmid.
    Depositor
    Dorus Gadella
    Publication
    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Gbeta-2A-cpV-Ggamma2
    Galphai2-mTurquoise2
    Tags
    ciruclar permutated Venus (cp173V) and mTurquoise…
    Expression
    Mammalian
    Promoter
    CMV and CMV (IRES)
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    69624-plasmid-map-sequence-id-123358

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  6. pAAV Syn ChR E90R-D156N-T159C 2A tDimer

    Plasmid
    #52494
    Purpose
    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)
    Depositor
    Thomas Oertner
    Publication
    Wietek et al Science. 2014 Mar 27.
    Inserts
    Channelrhodopsin-2
    red fluorescent protein
    Use
    AAV
    Expression
    Mammalian
    Mutation
    E90R, D156N, T159C
    Promoter
    human synapsin-1 and ribosomal skip sequence
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    52494-plasmid-map-sequence-id-81429

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  9. p2attNG-H11-long

    Plasmid
    #51545
    Purpose
    p2attNG landing pad plasmid with 5 and 3kb homology arms to the human H11 locus.
    Depositor
    Michele Calos
    Publication
    Zhu et al Nucleic Acids Res. 2013 Dec 4.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP4m
    Use
    Human targeting
    Expression
    Mammalian
    Available since
    March 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    51545-plasmid-map-sequence-id-78773

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  12. pAAV-CaMKII-ChrimsonR-tdTomato

    Plasmid
    #99231
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.
    Depositor
    Edward Boyden
    Publication
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    CaMKII
    Available since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    99231-plasmid-map-sequence-id-219823

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  15. COVID-SARS2 NSP14/10

    Plasmid
    #159613
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10
    Depositor
    Opher Gileadi
    Publication
    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650
    Inserts
    Covid-SARS2 Nsp14 (ORF1ab COVID-SARS2, Synthetic)
    Covid-SARS2 Nsp10
    Tags
    His6, TEV cleavage site and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    159613-plasmid-map-sequence-id-311056

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  16. pGEX6P1‐hsRNASEH2BCA

    Plasmid
    #108692
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme
    Depositor
    Andrew Jackson, Martin Reijns
    Publication
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A (RNASEH2A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon a…
    Promoter
    tac
    Available since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    108692-plasmid-map-sequence-id-208976

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Showing: 3461 - 3480 of 18346 results