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Nluc CD9

Plasmid

#247322

Purpose

This plasmid can be used to quantify CD9 by luminescence signal upon providing substrate, in particular in the secretome, especially in the extracellular vesicles of cells expressing this construct.

Depositor

Clotilde Thery

Article

Grisard et al J Extracell Vesicles. 2022 Jul;11(7):e12

Insert

CD9 (CD9 Human)

Use

Luciferase

Tags

Nanoluciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-CDK4

Plasmid

#172612

Purpose

Expresses HA-tagged CDK4 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK4 (CDK4 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pIG-829_HA-GD2-28z_CAR_JUN_Retroviral

Plasmid

#207506

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

JUN, HA-GD2-28z_CAR (JUN Human)

Use

Retroviral

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-THRA-RFP-EGFP

Plasmid

#228537

Purpose

Bichromatic fluorescent reporter of THRA isoform 1 and isoform 2 splicing in mammalian cells

Depositor

Markus Schuelke

Article

Graceffo et al Int J Mol Sci. 2024 Dec 17;25(24):13512.

Insert

Thyroid Hormone Receptor Alpha (THRA Human)

Tags

eGFP and tagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pIG-828_HA-GD2-28z_CAR_BATF_Retroviral

Plasmid

#207505

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

BATF, HA-GD2-28z_CAR (BATF Human)

Use

Retroviral

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/T0-Flag-53BP1

Plasmid

#52507

Purpose

mammalian expression vector of 53BP1

Depositor

Daniel Durocher

Article

Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

Insert

53BP1 (TP53BP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

V128A and siRNA resistant sequence: ACGCGCTGACGAC…

Promoter

CMV/TO

Available Since

March 26, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pTol2-UAS-zmiRFP

Plasmid

#108415

Purpose

Codon-optimized monomeric near-infrared fluorescent protein miRFP in zebrafish expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

zmiRFP

Use

Zebrafish

Promoter

UAS

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Archon1-KGC-EGFP

Plasmid

#108418

Purpose

AAV production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND2

Plasmid

#172629

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pF CAG BFP2 IRES neo

Plasmid

#108175

Purpose

Expresses a blue fluorescent protein (BFP2) in mammalian cells along with conferring resistance to G418

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

Blue fluorescent protein (BFP2)

Use

Lentiviral; Fluorescent protein

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-CDK2

Plasmid

#172613

Purpose

Expresses HA-tagged CDK2 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK2 (CDK2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-EF1a-Archon2-KGC-EGFP-ER2

Plasmid

#108426

Purpose

Lentivirus production plasmid encoding for Archon2 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon2-KGC-EGFP-ER2

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-EF1-IRES-NEO-Control

Plasmid

#80757

Purpose

Transposon Reporter

Depositor

Alex Kentsis

Article

Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

Insert

NeoR (neoR Synthetic)

Promoter

EF1-IRES from PB-EF1-IRES-NEO

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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px459-AMBRA1 #3

Plasmid

#172606

Purpose

Expresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-Puro

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

gRNA against AMBRA1 (AMBRA1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pmiRFP-beta-Actin-C-19

Plasmid

#108412

Purpose

Beta-actin fused to monomeric near-infrared fluorescent protein miRFP

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

beta-Actin

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSF143-wArchon2-KGC-EGFP-ER2

Plasmid

#108430

Purpose

Codon-optimized Archon2 fluorescent voltage reporter in C.elegans expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

wArchon2-KGC-EGFP-ER2

Expression

Worm

Promoter

flp-18

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND3

Plasmid

#172623

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D3 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSL012_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

Plasmid

#179429

Purpose

Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-5kb-mch

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CCND1(T286A)

Plasmid

#172631

Purpose

Expresses untagged cyclin D1(T286A) in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Expression

Mammalian

Mutation

Thr286Ala

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDonor MAFA-P2A-tdTomato

Plasmid

#246654

Purpose

Donor plasmid for Crispr/Cas9 gene editing of the human MAFA locus

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

P2A-tdTomato flanked by MAFA homology regions (MAFA Synthetic, Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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