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We narrowed to 14,357 results for: cas9

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Showing: 3481 - 3500 of 14357 results

  1. pdCas9-GFP-VPR

    Plasmid
    #181907
    Purpose
    Fluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-Rta
    Depositor
    Karsten Rippe
    Publication
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VPR
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181907-plasmid-map-sequence-id-359690

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  2. hPLD3-sgRNA-Cas9_mcherry

    Plasmid
    #199342
    Purpose
    encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Promoter
    human U6
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199342-plasmid-map-sequence-id-390619

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  3. dCas9-NS3-NLS/VPR

    Plasmid
    #112244
    Purpose
    Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.
    Depositor
    John Ngo
    Publication
    Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-NS3-NLS/VPR
    Tags
    AU1 (N term on NS3) and HA (C term on NS3)
    Expression
    Mammalian
    Mutation
    NS3 mutant T54A
    Promoter
    CMV
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112244-plasmid-map-sequence-id-216918

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  4. pSUSL001T:pTRE3G-dCas9-2xKRAB-p2a-tdTomato

    Plasmid
    #209298
    Purpose
    A lentiviral vector with tetracycline-inducible system to control expression of S. aureus dCas9 (with tdTomato) to silencing specific genes due to interference with gene transcription machinery.
    Depositor
    Birgitt Schuele
    Publication
    Sastre et al Sci Rep. 2023 Oct 18;13(1):17796. doi: 1
    Inserts
    dCas9
    tdTomato
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    tetracycline-inducible promoter (TRE3G)
    Available since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    209298-plasmid-map-sequence-id-414164

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  7. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Publication
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124451-plasmid-map-sequence-id-257763

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  9. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118158
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118158-plasmid-map-sequence-id-240404

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  10. pDD428

    Plasmid
    #202081
    Purpose
    Cas9/sgRNA plasmid targeting the C-terminus of Myh9
    Depositor
    Daniel Dickinson
    Publication
    Shi et al Development. 2023 May 15;150(10):dev2013
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))
    Use
    CRISPR
    Promoter
    EF1a
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202081-plasmid-map-sequence-id-399529

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  14. pUC19-D3UFL8

    Plasmid
    #103095
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    D3UFL8(Cas9 coding gene from Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103095-plasmid-map-sequence-id-201805

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  15. PEA1-Puro-HEK3 CTT ins

    Plasmid
    #171996
    Purpose
    Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171996-plasmid-map-sequence-id-338997

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  17. pMB1610_pRR-Puro

    Plasmid
    #65853
    Purpose
    Puromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9
    Depositor
    Marc Bühler
    Publication
    Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    split puromycin N-acetyltransferase coding sequence
    Use
    CRISPR and TALEN
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65853-plasmid-map-sequence-id-112015

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  19. Intron-Tagging-pX330-Cas9-Blast

    Plasmid
    #159741
    Purpose
    Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.
    Depositor
    Stefan Kubicek
    Publication
    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    donor plasmid targeting sgRNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    159741-plasmid-map-sequence-id-314866

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Showing: 3481 - 3500 of 14357 results